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Inheritance of chloroplast and mitochondrial DNA in Picea and composition of hybrids from introgression zones

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Summary

The cloning of white spruce (Picea glauca) mitochondrial DNA homologous to the cytochrome oxidase II and ATPaseα genes of maize is described. These probes were used to define restriction fragment length polymorphisms which distinguish the white, Engelmann (P. engelmannii) and Sitka spruce (P. sitchensis) populations that occur in British Columbia. Analysis of progeny from crosses between the species revealed that mitochondrial DNA was maternally inherited in all cases (32 progeny from five independent crosses). The inheritance of chloroplast DNA was determined using a probe described previously; in this case, all progeny exhibited paternal inheritance (27 progeny from four crosses). Mitochondrial and chloroplast probes were used to test trees from zones of introgression between coastal (Sitka) and interior spruces (white and Engelmann). In most cases mitochondria and chloroplasts within individuals were contributed by different species. The data shows that there is a significant Sitka spruce component in trees east of the coastal watershed in British Columbia.

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Authors and Affiliations

  1. Forest Biotechnology Centre, British Columbia Research Corporation, 3650 Wesbrook Mall, V6S 2L2, Vancouver, B.C., Canada

    B. C. S. Sutton, D. J. Flanagan, J. R. Gawley & C. H. Newton

  2. Forest Science Department, University of British Columbia, 2357 Main Mall, V6T 1W5, Vancouver, B.C., Canada

    B. C. S. Sutton, D. T. Lester & Y. A. El-Kassaby

  3. Saanich Forestry Centre, Canadian Pacific Forest Products Ltd, 8067 East Saanich Rd, V0S 1M0, RR1, Saanichton, B.C., Canada

    Y. A. El-Kassaby

Authors
  1. B. C. S. Sutton
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  2. D. J. Flanagan
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  3. J. R. Gawley
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  4. C. H. Newton
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  5. D. T. Lester
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  6. Y. A. El-Kassaby
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Communicated by P.M.A. Tigerstedt

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Sutton, B.C.S., Flanagan, D.J., Gawley, J.R. et al. Inheritance of chloroplast and mitochondrial DNA in Picea and composition of hybrids from introgression zones. Theoret. Appl. Genetics 82, 242–248 (1991). https://doi.org/10.1007/BF00226220

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  • DOI: https://doi.org/10.1007/BF00226220

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