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Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells

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Summary

The ability of selected vasoactive agents to influence cyclic AMP levels of confluent, early-passaged bovine calf aortic and pulmonary artery endothelial cells was investigated. Among the agents tested, only the catecholamines (isoproterenol, epinephrine, nonrepinephrine) and prostaglandins (PGE1, PGE2, PGF2a) resulted consistently in increased cyclic AMP production in both cell populations. The degree of cyclic AMP stimulation obtained with other vasoactive compounds (angiotensins I and II, bradykinin, and serotonin) tended to be either very small or difficult to reproduce. Isoproterenol stimulation was blocked completely by propanolol, a β-blocking agent, but not by phentolamine, an α-blocking agent. These results reveal that bovine calf aortic and pulmonary artery endothelial cells are responsive to catecholamines and prostaglandins, and therefore presumably possess both sensitive adenylate cyclases and plasma membrane receptors for these compounds.

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Authors and Affiliations

  1. Department of Biochemistry and Division of Surgery, Boston University School of Medicine, 908 Instructional Building, 80 East Concord Street, 02118, Boston, Massachusetts

    Joseph S. Makarski

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  1. Joseph S. Makarski
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This work was supported by a Young Investigator Grant HL-21189 from the National Institutes of Health, United States Public Health Service.

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Makarski, J.S. Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells. In Vitro 17, 450–458 (1981). https://doi.org/10.1007/BF02626746

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  • DOI: https://doi.org/10.1007/BF02626746

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