Biomolecular condensates are emerging as hubs of splicing regulation. This Review discusses the modulation of condensate functions through alternative splicing, regulation of (co-transcriptional) splicing at condensates and the involvement of these condensates in human diseases.

Ferroptosis is a non-apoptotic, iron-dependent cell death mechanism driven by plasma membrane lipid peroxidation and subsequent plasma membrane rupture. Various cellular compartments and organelles contribute to regulating susceptibility to ferroptosis. This regulation involves a plethora of mechanisms centred on iron metabolism and storage, lipid metabolism, and redox balance.

C. David Allis’s discovery of the first histone acetyltransferase from Tetrahymena exemplifies an approach that continues to evolve and now has a crucial role in drug development.

Senescent cells in the amputated head of the cnidarian Hydractinia symbiolongicarpus drive the reprogramming of somatic cells into pluripotent stem cells, which are required for full body regeneration.

Removal of different types of senescent cells can be either beneficial or detrimental to health, with potential consequences to senotherapies.

In this Tools of the Trade article, Duran (Gil lab) describes the development of novel machine learning algorithms that enable the detection of senescent cells in vitro and in diverse tissues based solely on nuclear morphologeny analysis.

James Kirkland discusses how work by Norman Sharpless and colleagues, published in 2004, paved the way for the development of senolytics, which are now in early phase clinical trials for the treatment of multiple disorders.

Raffaella Di Micco discusses the importance of a 2020 study in which CAR-T cells were engineered to eliminate senescent cells.