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Formation and dissolution of hen egg white lysozyme amyloid fibrils in protic ionic liquids

Chem Commun (Camb). 2009 Mar 7:(9):1046-8. doi: 10.1039/b817590j. Epub 2009 Feb 2.

Abstract

The formation of amyloid fibrils from non-disease-related proteins demonstrates that any protein can adopt this "rogue" form; we show that it is possible to use protic ionic liquids to fibrilize hen egg white lysozyme, and then subsequently to dissolve the fibrils with up to 72% restoration of enzymatic activity.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amyloid / chemistry*
  • Amyloid / metabolism
  • Amyloid / ultrastructure
  • Animals
  • Chickens
  • Circular Dichroism
  • Ionic Liquids / chemistry*
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Muramidase / ultrastructure
  • Protein Renaturation

Substances

  • Amyloid
  • Ionic Liquids
  • hen egg lysozyme
  • Muramidase