Adnan Menderes Universitesi Ziraat Fakultesi Dergisi, Dec 21, 2012
Bu calismada durgun su yontemiyle, karanlik ortamda, farkli stok miktarlari uzerine calisilmistir... more Bu calismada durgun su yontemiyle, karanlik ortamda, farkli stok miktarlari uzerine calisilmistir. Calismada 10 L'lik su sirkulasyonu olmayan inkibatorler kullanilmistir. 4 degisik stok miktari mevcuttur. Bu stoklar 250, 500, 750 ve 1000 yum/L'dir. Kuluckalama islemi yapildiktan sonra oksijen ve sicaklik degerleri alinmistir. Yumurtalar 39 saatte acilim gostermis ve yumurtadan cikan larvalar hacimsel metotla sayilmis ve yasama yuzdeleri hesaplanmistir. Buna gore en iyi acilim orani 250 adet/L'ye % 92 acilim gosteren A gurubunda tespit edilmis, bunu 500 adet/L'ye % 86 acilim gosteren B gurubu takip etmistir. Acilim orani C ve D guruplarinda ise % 62 ile % 59 arasinda degisim gostermistir. Durgun su yontemi ile yapilan calismada bu acilim yuzdeleri bize stok miktari artikca acilimin dustugunu belirtmektedir.
The ontogenesis of main pancreatic and intestinal enzymes was investigated in the recent promisin... more The ontogenesis of main pancreatic and intestinal enzymes was investigated in the recent promising Mediterranean candidate species of meagre, Argyrosomus regius, during larval development until 40 days after hatching (DAH). The green-water technique was carried out for larval rearing. Whole-body homogenates were used for enzymatic analysis in larvae younger than 15 DAH; after this date, older larvae were dissected into two segments as pancreatic and intestinal segment. Trypsin was detected as early as hatching and sharply increased concurrently with age and exogenous feeding 15 DAH, but constant decline was observed until the end of experiment. Amylase was determined at 2 DAH and sharply increased 10 DAH. Then, slight decreases were found between 10 and 15 DAH, and then slow alterations were continued until the end of the experiment. Lipase was firstly measured on day 3; then, sudden decline was observed between 20 and 25 DAH. After this date, slow fluctuations were maintained until the end of the experiment. Pepsin was firstly assayed 15 DAH related to gastric gland secretion and sharply increased 30 DAH. Then, it slowly varied until end of the experiment. Enzymes of brush border membranes, alkaline phosphatase and aminopeptidase N showed similar pattern on specific activities during the first 10 days. Thereafter, while specific activity of alkaline phosphatase slightly decreased 15 and fluctuated until 20 DAH, aminopeptidase N activity slowly increased 20 DAH. Then, activity of alkaline phosphatase and aminopeptidase N constantly increased 30 DAH, indicating maturation of the intestinal digestive process, and also, these activities continued to slowly increase until the end of the experiment. The specific activity of cytosolic peptidase, leucine-alanine peptidase, smoothly increased on day 8, then fluctuated until 15 DAH. After this date, in contrast to enzymes of brush border membranes, it sharply decreased 25 DAH and continued to gradually decline until the end of the experiment. These converse expressions were indicative of a maturation of enterocytes and the transition of an adult mode of digestion.
In this study, bone and cartilage deformations occurred in gilthead sea bream ( Sparus aurata ) l... more In this study, bone and cartilage deformations occurred in gilthead sea bream ( Sparus aurata ) larval development were investigated in under culture conditions. Whole larval period was examined in three stages based on notochord curve. In sea bream larvae, total 1074 larvae were examined and 1926 deformations were detected in 389 larvae. Deformation rate was calculated as 36% in
Morphological development and allometric growth were investigated in the gilthead seabream Sparus... more Morphological development and allometric growth were investigated in the gilthead seabream Sparus aurata during larval development until the end of the weaning period on day 41. Fertilized eggs had a diameter of 1.01 ± 0.02 mm (mean ± SE) and a lipid vesicle diameter of 0.227 ± 0.021 mm. Mean total length (TL) was 2.68 ± 0.04 mm for newly
Adnan Menderes Universitesi Ziraat Fakultesi Dergisi, Dec 21, 2012
Bu calismada durgun su yontemiyle, karanlik ortamda, farkli stok miktarlari uzerine calisilmistir... more Bu calismada durgun su yontemiyle, karanlik ortamda, farkli stok miktarlari uzerine calisilmistir. Calismada 10 L'lik su sirkulasyonu olmayan inkibatorler kullanilmistir. 4 degisik stok miktari mevcuttur. Bu stoklar 250, 500, 750 ve 1000 yum/L'dir. Kuluckalama islemi yapildiktan sonra oksijen ve sicaklik degerleri alinmistir. Yumurtalar 39 saatte acilim gostermis ve yumurtadan cikan larvalar hacimsel metotla sayilmis ve yasama yuzdeleri hesaplanmistir. Buna gore en iyi acilim orani 250 adet/L'ye % 92 acilim gosteren A gurubunda tespit edilmis, bunu 500 adet/L'ye % 86 acilim gosteren B gurubu takip etmistir. Acilim orani C ve D guruplarinda ise % 62 ile % 59 arasinda degisim gostermistir. Durgun su yontemi ile yapilan calismada bu acilim yuzdeleri bize stok miktari artikca acilimin dustugunu belirtmektedir.
The ontogenesis of main pancreatic and intestinal enzymes was investigated in the recent promisin... more The ontogenesis of main pancreatic and intestinal enzymes was investigated in the recent promising Mediterranean candidate species of meagre, Argyrosomus regius, during larval development until 40 days after hatching (DAH). The green-water technique was carried out for larval rearing. Whole-body homogenates were used for enzymatic analysis in larvae younger than 15 DAH; after this date, older larvae were dissected into two segments as pancreatic and intestinal segment. Trypsin was detected as early as hatching and sharply increased concurrently with age and exogenous feeding 15 DAH, but constant decline was observed until the end of experiment. Amylase was determined at 2 DAH and sharply increased 10 DAH. Then, slight decreases were found between 10 and 15 DAH, and then slow alterations were continued until the end of the experiment. Lipase was firstly measured on day 3; then, sudden decline was observed between 20 and 25 DAH. After this date, slow fluctuations were maintained until the end of the experiment. Pepsin was firstly assayed 15 DAH related to gastric gland secretion and sharply increased 30 DAH. Then, it slowly varied until end of the experiment. Enzymes of brush border membranes, alkaline phosphatase and aminopeptidase N showed similar pattern on specific activities during the first 10 days. Thereafter, while specific activity of alkaline phosphatase slightly decreased 15 and fluctuated until 20 DAH, aminopeptidase N activity slowly increased 20 DAH. Then, activity of alkaline phosphatase and aminopeptidase N constantly increased 30 DAH, indicating maturation of the intestinal digestive process, and also, these activities continued to slowly increase until the end of the experiment. The specific activity of cytosolic peptidase, leucine-alanine peptidase, smoothly increased on day 8, then fluctuated until 15 DAH. After this date, in contrast to enzymes of brush border membranes, it sharply decreased 25 DAH and continued to gradually decline until the end of the experiment. These converse expressions were indicative of a maturation of enterocytes and the transition of an adult mode of digestion.
In this study, bone and cartilage deformations occurred in gilthead sea bream ( Sparus aurata ) l... more In this study, bone and cartilage deformations occurred in gilthead sea bream ( Sparus aurata ) larval development were investigated in under culture conditions. Whole larval period was examined in three stages based on notochord curve. In sea bream larvae, total 1074 larvae were examined and 1926 deformations were detected in 389 larvae. Deformation rate was calculated as 36% in
Morphological development and allometric growth were investigated in the gilthead seabream Sparus... more Morphological development and allometric growth were investigated in the gilthead seabream Sparus aurata during larval development until the end of the weaning period on day 41. Fertilized eggs had a diameter of 1.01 ± 0.02 mm (mean ± SE) and a lipid vesicle diameter of 0.227 ± 0.021 mm. Mean total length (TL) was 2.68 ± 0.04 mm for newly
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