Egg parasitoid Trichogramma strain (TNV) received from Kharga Oasis, New Valley Governorate, Egyp... more Egg parasitoid Trichogramma strain (TNV) received from Kharga Oasis, New Valley Governorate, Egypt, is used in controlling certain date palm lepidopteran insect pests. The morphological features of adult wasps and genetically identification of the strain was carried out. Scanning electron microscopy (SEM) and light microscope were used for studying morphology of male and female adults and measurements. Data revealed that body length of male was shorter than female. However, adult parasitoid body length of TNV was longer than the species, Trichogramma evanescens West. studied by several authors. In addition, external morphology of antennae, wings, and male genitalia were differed in TNV Trichogramma. The strain TNV identification was based on the molecular genetic techniques using PCR amplification and sequencing of the internal transcribed spacer two (ITS2) region of the rDNA. The BLAST result and phylogenetic analysis of the ITS2 region of rDNA sequence revealed that the strain TNV is closely related to Trichogramma turkestanica. Obtained results confirmed that the Trichogramma sp., used as a biological control in the New Valley, is T. turkestanica and not T. evanescens.
Egg parasitoid Trichogramma strain (TNV) received from Kharga Oasis, New Valley Governorate, Egyp... more Egg parasitoid Trichogramma strain (TNV) received from Kharga Oasis, New Valley Governorate, Egypt, is used in controlling certain date palm lepidopteran insect pests. The morphological features of adult wasps and genetically identification of the strain was carried out. Scanning electron microscopy (SEM) and light microscope were used for studying morphology of male and female adults and measurements. Data revealed that body length of male was shorter than female. However, adult parasitoid body length of TNV was longer than the species, Trichogramma evanescens West. studied by several authors. In addition, external morphology of antennae, wings, and male genitalia were differed in TNV Trichogramma. The strain TNV identification was based on the molecular genetic techniques using PCR amplification and sequencing of the internal transcribed spacer two (ITS2) region of the rDNA. The BLAST result and phylogenetic analysis of the ITS2 region of rDNA sequence revealed that the strain TNV is closely related to Trichogramma turkestanica. Obtained results confirmed that the Trichogramma sp., used as a biological control in the New Valley, is T. turkestanica and not T. evanescens.
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