This report describes a fully elaborated and validated method for quantitation of the hydrocarbon... more This report describes a fully elaborated and validated method for quantitation of the hydrocarbons n-propane,/so-butane, and n-butane in blood samples. The newly developed analytical procedure is suitable for both emergency cases and forensic medicine investigations. Its practical applicability is illustrated with a forensic blood sample after acute inhalative intoxication with lighter fluid; case history and toxicological findings are included. Identification and quantitation of the analytes were performed using static headspace extraction combined with gas chromatography-mass pectrometry. In order to reconcile the large gas volumes injected (0.5 mL) with the narrowbore capillary column and thus achieve preconcentration, cold trapping on a Tenax sorbent followed by flash desorption was applied. Adequate retention and separation were achieved isothermally at 35 ~ on a
Background Autologous facial fat grafting has gained popularity in recent years and is considered... more Background Autologous facial fat grafting has gained popularity in recent years and is considered to be safe. This paper presents the case of a patient who died due to massive cerebral microfat embolism after facial fat grafting. Objectives The aim of this study was to raise awareness and provide more evidence on the prevention and treatment of this potentially lethal complication of facial fat grafting. Methods A detailed report was made of the case. Two online databases were searched for similar cases of facial fat embolism resulting in neurologic and/or visual symptoms. Thereafter a literature search was conducted to verify the etiology, current treatment options, and preventive measures. Results Forty-nine cases with similar events were found in the literature. The most common injected area was the glabella (36.1%), and an average of 16.7 mL fat was injected. The main complications were visual impairment, with 88.5% of cases resulting in permanent monocular blindness, and neurol...
The use of 3T magnetic resonance imaging (MRI) of the clavicle in forensic bone age determination... more The use of 3T magnetic resonance imaging (MRI) of the clavicle in forensic bone age determination was prospectively examined and compared with plain radiography. Four MRI sequences and three radiographs of 121 healthy subjects between 11 and 30 were studied by two observers. The number of images assessable for bone age determination was lower for plain radiography (PA: 68.7%; oblique: 97.5%) compared with MRI (VIBE: 99.0%). Concerning the subjective level of difficulty to assess bone age, the observers found it easier to assess bone age on MRI than on radiography. The developmental stages of the clavicle, as used on plain radiography, were transferable to MRI. Especially the VIBE gradient echo sequence provided an excellent depiction of the growth cartilage and ossification centre with a slice thickness of 0.9 mm and only a 4-min acquisition time. When the developmental stages were assigned, less variability between the observers was seen on MRI, compared with plain radiography. We conclude that 3T MRI provides high resolution, cross-sectional images of the maturation of the clavicle without ionising radiation in a very short time, allowing more accurate determination of bone age than plain radiography.
In this work, a new methodology for direct determination of Al in whole blood samples by means of... more In this work, a new methodology for direct determination of Al in whole blood samples by means of high-resolution continuum source graphite furnace molecular absorption spectrometry has been developed, based on the formation of the AlF diatomic molecule in the graphite furnace and the subsequent monitoring of its molecular absorption. The proposed methodology provides an alternative method to conventional atomic absorption, solving most of the problems related to the latter technique, particularly matrix effects, providing a straightforward alternative for blood analysis. The addition of NH4F·HF, which is required for promotion of the AlF molecule, was found to improve sample matrix removal for whole blood samples, whether they contain EDTA or heparin as anticoagulant agents. Besides minimizing residues in the graphite platform, this circumstance enabled the use of aqueous standards to build a calibration curve, avoiding the need for the cumbersome method of standard additions, whil...
This report describes a fully elaborated and validated method for quantitation of the hydrocarbon... more This report describes a fully elaborated and validated method for quantitation of the hydrocarbons n-propane,/so-butane, and n-butane in blood samples. The newly developed analytical procedure is suitable for both emergency cases and forensic medicine investigations. Its practical applicability is illustrated with a forensic blood sample after acute inhalative intoxication with lighter fluid; case history and toxicological findings are included. Identification and quantitation of the analytes were performed using static headspace extraction combined with gas chromatography-mass pectrometry. In order to reconcile the large gas volumes injected (0.5 mL) with the narrowbore capillary column and thus achieve preconcentration, cold trapping on a Tenax sorbent followed by flash desorption was applied. Adequate retention and separation were achieved isothermally at 35 ~ on a
Background Autologous facial fat grafting has gained popularity in recent years and is considered... more Background Autologous facial fat grafting has gained popularity in recent years and is considered to be safe. This paper presents the case of a patient who died due to massive cerebral microfat embolism after facial fat grafting. Objectives The aim of this study was to raise awareness and provide more evidence on the prevention and treatment of this potentially lethal complication of facial fat grafting. Methods A detailed report was made of the case. Two online databases were searched for similar cases of facial fat embolism resulting in neurologic and/or visual symptoms. Thereafter a literature search was conducted to verify the etiology, current treatment options, and preventive measures. Results Forty-nine cases with similar events were found in the literature. The most common injected area was the glabella (36.1%), and an average of 16.7 mL fat was injected. The main complications were visual impairment, with 88.5% of cases resulting in permanent monocular blindness, and neurol...
The use of 3T magnetic resonance imaging (MRI) of the clavicle in forensic bone age determination... more The use of 3T magnetic resonance imaging (MRI) of the clavicle in forensic bone age determination was prospectively examined and compared with plain radiography. Four MRI sequences and three radiographs of 121 healthy subjects between 11 and 30 were studied by two observers. The number of images assessable for bone age determination was lower for plain radiography (PA: 68.7%; oblique: 97.5%) compared with MRI (VIBE: 99.0%). Concerning the subjective level of difficulty to assess bone age, the observers found it easier to assess bone age on MRI than on radiography. The developmental stages of the clavicle, as used on plain radiography, were transferable to MRI. Especially the VIBE gradient echo sequence provided an excellent depiction of the growth cartilage and ossification centre with a slice thickness of 0.9 mm and only a 4-min acquisition time. When the developmental stages were assigned, less variability between the observers was seen on MRI, compared with plain radiography. We conclude that 3T MRI provides high resolution, cross-sectional images of the maturation of the clavicle without ionising radiation in a very short time, allowing more accurate determination of bone age than plain radiography.
In this work, a new methodology for direct determination of Al in whole blood samples by means of... more In this work, a new methodology for direct determination of Al in whole blood samples by means of high-resolution continuum source graphite furnace molecular absorption spectrometry has been developed, based on the formation of the AlF diatomic molecule in the graphite furnace and the subsequent monitoring of its molecular absorption. The proposed methodology provides an alternative method to conventional atomic absorption, solving most of the problems related to the latter technique, particularly matrix effects, providing a straightforward alternative for blood analysis. The addition of NH4F·HF, which is required for promotion of the AlF molecule, was found to improve sample matrix removal for whole blood samples, whether they contain EDTA or heparin as anticoagulant agents. Besides minimizing residues in the graphite platform, this circumstance enabled the use of aqueous standards to build a calibration curve, avoiding the need for the cumbersome method of standard additions, whil...
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