The current study evaluated the effect of various solvents extract from Nerium oleander leaves on... more The current study evaluated the effect of various solvents extract from Nerium oleander leaves on developing stages in Heliothis armigera. To study the reproductive aspects of Heliothis armigera, leaf extract of Nerium oleander was prepared in chloroform and methanol solvents. After 24 and 96 hours of treatment, both chloroform and methanol extract were interrupt their life cycle and the notable changes had been observed such as increased mortality and emergence of adults were decreased.
An alkaliphilic actinomycetes was sequestered from a desert soil sample of Lonar, District Buldha... more An alkaliphilic actinomycetes was sequestered from a desert soil sample of Lonar, District Buldhana. The isolate was detected to produce; white, grey, milky white (cotton) color colonies are obtained from soil sample. These strain produced aerial and substrate mycelium comprising of chain or smooth spore. The colonical growth of strain varied from yellow to grey. All the isolate were later purified and imperiled to a few phosphatic enzymatic screening. Results indicate that number of isolates showed the ability to solubilize phosphate.
The concentrations of eight essential minerals Ca, Cr, Co Cu, Fe, Mn, Ag, Zn and five toxic trace... more The concentrations of eight essential minerals Ca, Cr, Co Cu, Fe, Mn, Ag, Zn and five toxic trace metals As, Cd, Pb, Hg and Ni were determined in blended raw honey, soil, leaf and flowers harvested from different locations of Kannad taluka. Result reveals that all honey samples from agricultural and road side area was contaminated with toxic trace metals. The honey samples harvested from forest area have no or lower concentrations of toxic trace metals than the permissible limits set by Indian standards (2010). The concentrations of these metals in honey samples investigated do not pose any serious concern to human health except honey harvested from road side area. Hence, the honey produced from Kannad taluka can be considered nutritionally safe and represents good quality. The present data also indicate that the levels of toxic trace metals in honey are comparatively more in roadway area than agricultural and forest areas. The essential minerals in soil, leaf and flower samples comparatively more in agricultural area than roadway and forest areas. The toxic trace metals are more in roadway sides than agricultural and forest areas.
The present study deals with the enzyme activity of honey samples harvested from three different ... more The present study deals with the enzyme activity of honey samples harvested from three different locations of Kannad region of Aurangabad district (M. S.), India. Diastase and invertase activity were analyzed by using Schade et al., (1958) and Siegenthaler (1977) method respectively. Results clearly indicate that the honey harvested from agricultural and forest area shows highest enzyme activity than road side area. The values of enzymes in honey were varied from location to location. This study also clearly demonstrates thin viat honey harvested from Kannad region were fresh and unheated because the honey samples have more enzyme activity. The values of diastase and invertase were within the quality regulation limits proposed by Codex standards.
The present study deals with the effect of storage on Apis mellifera honey quality parameters har... more The present study deals with the effect of storage on Apis mellifera honey quality parameters harvested from Kannad taluka of Aurangabad district (M.S.), India. Honey quality parameters like pH, electrical conductivity, moisture content, proline content, HMF content, invertase and diastase activity were measured by AOAC method (2000) at intervals of each six month for the period of 24 month. Student's T-Test was applied to the analyzed data. Results clearly indicates that the pH, electrical conductivity, moisture content and HMF content were increased with the increasing of storage period and concentration of proline, invertase and diastase number were decreased with the increasing of storage time limit. The said physicochemical parameters of honey sample were found statistically significant according to their storage time limit. The obtained data also clearly demonstrate that the invertase is more sensitive than the diastase and HMF content to the storage as well as heat treatment of honey.
The aim of present investigation was to study the antifungal activity of raw honey of three honey... more The aim of present investigation was to study the antifungal activity of raw honey of three honeybee species collected from Kannad taluka of Aurangabad district. Honey samples were collected and assayed against three fungal species by using Agar well diffusion method. All honey samples showed sensitivity against all the fungal species. The zone of inhibition clearly showed that honey obtained from Apis florea and Apis cerana indica having highest antifungal activity, while honey sample of Apis dorsata showed minimum antifungal activity. The most sensitive fungi were Aspergillus and Fusarium, while Alternaria spp. was less sensitive to the inhibitory action of honey.
The current study evaluated the effect of various solvents extract from Nerium oleander leaves on... more The current study evaluated the effect of various solvents extract from Nerium oleander leaves on developing stages in Heliothis armigera. To study the reproductive aspects of Heliothis armigera, leaf extract of Nerium oleander was prepared in chloroform and methanol solvents. After 24 and 96 hours of treatment, both chloroform and methanol extract were interrupt their life cycle and the notable changes had been observed such as increased mortality and emergence of adults were decreased.
Jawalkar, N.B., S.P. Zambare and M.I. Al Ghannoum. 2021. The Potency of Six Medicinal Plant Extra... more Jawalkar, N.B., S.P. Zambare and M.I. Al Ghannoum. 2021. The Potency of Six Medicinal Plant Extracts Against the Stored Grain Insect Pest Sitophilus granarius L. Arab Journal of Plant Protection, 39(4): 323-328. https://doi.org/10.22268/AJPP-039.4.323328 This study was conducted on six medicinal plants viz., Vitex negundo (leaves), Xanthium strumarium, Caesalpinia bonduc, Mucuna pruriens, Moringa oleifera (seed kernels), Tagetes erecta (petals) for their bio-insecticidal activity. The powders of various parts of plants were extracted using the MARS6 microwave acid digestion system. Three different concentrations (20, 30, and 40%) of plant extracts were tested against granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae) for their bio-insecticidal activities under laboratory conditions. The results of statistical analysis showed a good performance of all plant extracts, especially at the high concentrations of the extracts, where they showed different levels of insect m...
The insecticidal property of dry leaves of Vitex negundo against the Sitophilus granarius in the ... more The insecticidal property of dry leaves of Vitex negundo against the Sitophilus granarius in the laboratory at (28±3) 0 C and (78±3) % r. h. with Soxhlet extracted extracts of V. negundo leaves in ethanol, methanol, chloroform, acetone and n-Hexane were tested and found very effective to control the store grain insect pest. The biopesticidal effects of extracts in different solvents were compared by calculating LD10, LD50, LD90 and LD99 values for 96 hrs. The Probit analysis of data demonstrated that LD values for ethanol, methanol, chloroform, acetone and n-Hexane extracts were LD10-1.216, 1.141, 0.9298, 1.265, 0.5398 ml/Kg; LD50-2.997, 3.106, 2.490, 3.727, 1.302 ml/Kg; LD90-7.392, 8.457, 6.667, 10.98, 3.142 ml/Kg and LD99-15.43, 19.14, 14.88, 26.50, 6.439 ml/Kg respectively, for 96 hrs. These results demonstrated that the mortality increased with increase in concentration of extract and exposure time. There was nearly zero fecundity and next generation was not observed in treated grains. The extracts of V. negundo leaf may be of high value in grain storage against S. granarius, especially in subsistence agriculture where the plants are locally available to local farmers. Hence, we conclude that the ethanol, chloroform and n-Hexane extracts from V. negundo leaf are more effective and useful. It served as being a potential insecticidal agent and could be applicable to the management of populations of stored-product insects particularly against the infestation by granary weevil, S. granarius after proper dose formulation.
The insecticidal property of Datura stramonium seed extracts against the rice weevil, Sitophilus ... more The insecticidal property of Datura stramonium seed extracts against the rice weevil, Sitophilus oryzae was tested in the laboratory. The experiments were conducted at (28±3) 0 C and (78±3) % relative humidity. It was observed that Soxhlet's extracted extracts of D. stramonium seed in ethanol, chloroform and acetone were very effective to control the pest while extracts in methanol and n-hexane gave poor results. The probit analysis of data demonstrated that LD10, LD50 and LD90 values for ethanol, chloroform and acetone extracts was LD10 = 2.962, 3.080, 0.4752 ml/Kg, LD50 = 8.594, 7.379, 1.185 ml/Kg and LD90 = 24.94, 17.67, 2.957 ml/Kg respectively for 96 hrs. These results suggest that the mortality increased with increase in concentration as well as exposure time and the extracts of D. stramonium seed may be of high value in grain storage against S. oryzae, especially in subsistence agriculture where the plants are locally available to farmers with little resources. Hence, we conclude that extracts of D. stramonium seed served as being a potential insecticidal agent can be used against rice weevil, S. oryzae after proper dose formulation to prevent infestation in wheat and even other grains during storage.
Antimicrobial activity of five solvent extracts of local plants was evaluated in vitro, with four... more Antimicrobial activity of five solvent extracts of local plants was evaluated in vitro, with four strains of bacteria viz., Salmonella typhi, Escherichia coli, Shigella flexneri (gram-ve), Staphylococcus aureus (gram +ve) and four strains of fungi viz., Alternaria alternata, Penicillium notatum, Aspergillus niger, Penicillium digitatum microorganism. The in vitro antibacterial and antifungal activities were tested by agar disc diffusion method. The most active antibacterial plants were Vitex negundo, Tagetes erecta and antifungal plants were Xanthium strumarium, Vitex negundo and Tagetes erecta, respectively. The significant antimicrobial activities of potent extracts were compared with the standard antimicrobiotics, Ciprofloxacin and Fluconazole for bacteria as well as fungi respectively at 1 mg/ml concentration. Preliminary phytochemical analysis of V. negundo leaf, X. strumarium, M. pruriens, C. bonduc seed and T. erecta flower extracts generally revealed the presence of Alkaloids, Steroids, Terpenoids, Phenols, Saponins, Anthraquinones, Amino acids, Carotenoids, Flavonoids and Tannins at various concentrations. The results obtained in this study suggest that X. strumarium, V. negundo, T. erecta can be used in treating diseases caused by these test organisms.
The current study evaluated the effect of various solvents extract from Nerium oleander leaves on... more The current study evaluated the effect of various solvents extract from Nerium oleander leaves on developing stages in Heliothis armigera. To study the reproductive aspects of Heliothis armigera, leaf extract of Nerium oleander was prepared in chloroform and methanol solvents. After 24 and 96 hours of treatment, both chloroform and methanol extract were interrupt their life cycle and the notable changes had been observed such as increased mortality and emergence of adults were decreased.
An alkaliphilic actinomycetes was sequestered from a desert soil sample of Lonar, District Buldha... more An alkaliphilic actinomycetes was sequestered from a desert soil sample of Lonar, District Buldhana. The isolate was detected to produce; white, grey, milky white (cotton) color colonies are obtained from soil sample. These strain produced aerial and substrate mycelium comprising of chain or smooth spore. The colonical growth of strain varied from yellow to grey. All the isolate were later purified and imperiled to a few phosphatic enzymatic screening. Results indicate that number of isolates showed the ability to solubilize phosphate.
The concentrations of eight essential minerals Ca, Cr, Co Cu, Fe, Mn, Ag, Zn and five toxic trace... more The concentrations of eight essential minerals Ca, Cr, Co Cu, Fe, Mn, Ag, Zn and five toxic trace metals As, Cd, Pb, Hg and Ni were determined in blended raw honey, soil, leaf and flowers harvested from different locations of Kannad taluka. Result reveals that all honey samples from agricultural and road side area was contaminated with toxic trace metals. The honey samples harvested from forest area have no or lower concentrations of toxic trace metals than the permissible limits set by Indian standards (2010). The concentrations of these metals in honey samples investigated do not pose any serious concern to human health except honey harvested from road side area. Hence, the honey produced from Kannad taluka can be considered nutritionally safe and represents good quality. The present data also indicate that the levels of toxic trace metals in honey are comparatively more in roadway area than agricultural and forest areas. The essential minerals in soil, leaf and flower samples comparatively more in agricultural area than roadway and forest areas. The toxic trace metals are more in roadway sides than agricultural and forest areas.
The present study deals with the enzyme activity of honey samples harvested from three different ... more The present study deals with the enzyme activity of honey samples harvested from three different locations of Kannad region of Aurangabad district (M. S.), India. Diastase and invertase activity were analyzed by using Schade et al., (1958) and Siegenthaler (1977) method respectively. Results clearly indicate that the honey harvested from agricultural and forest area shows highest enzyme activity than road side area. The values of enzymes in honey were varied from location to location. This study also clearly demonstrates thin viat honey harvested from Kannad region were fresh and unheated because the honey samples have more enzyme activity. The values of diastase and invertase were within the quality regulation limits proposed by Codex standards.
The present study deals with the effect of storage on Apis mellifera honey quality parameters har... more The present study deals with the effect of storage on Apis mellifera honey quality parameters harvested from Kannad taluka of Aurangabad district (M.S.), India. Honey quality parameters like pH, electrical conductivity, moisture content, proline content, HMF content, invertase and diastase activity were measured by AOAC method (2000) at intervals of each six month for the period of 24 month. Student's T-Test was applied to the analyzed data. Results clearly indicates that the pH, electrical conductivity, moisture content and HMF content were increased with the increasing of storage period and concentration of proline, invertase and diastase number were decreased with the increasing of storage time limit. The said physicochemical parameters of honey sample were found statistically significant according to their storage time limit. The obtained data also clearly demonstrate that the invertase is more sensitive than the diastase and HMF content to the storage as well as heat treatment of honey.
The aim of present investigation was to study the antifungal activity of raw honey of three honey... more The aim of present investigation was to study the antifungal activity of raw honey of three honeybee species collected from Kannad taluka of Aurangabad district. Honey samples were collected and assayed against three fungal species by using Agar well diffusion method. All honey samples showed sensitivity against all the fungal species. The zone of inhibition clearly showed that honey obtained from Apis florea and Apis cerana indica having highest antifungal activity, while honey sample of Apis dorsata showed minimum antifungal activity. The most sensitive fungi were Aspergillus and Fusarium, while Alternaria spp. was less sensitive to the inhibitory action of honey.
The current study evaluated the effect of various solvents extract from Nerium oleander leaves on... more The current study evaluated the effect of various solvents extract from Nerium oleander leaves on developing stages in Heliothis armigera. To study the reproductive aspects of Heliothis armigera, leaf extract of Nerium oleander was prepared in chloroform and methanol solvents. After 24 and 96 hours of treatment, both chloroform and methanol extract were interrupt their life cycle and the notable changes had been observed such as increased mortality and emergence of adults were decreased.
Jawalkar, N.B., S.P. Zambare and M.I. Al Ghannoum. 2021. The Potency of Six Medicinal Plant Extra... more Jawalkar, N.B., S.P. Zambare and M.I. Al Ghannoum. 2021. The Potency of Six Medicinal Plant Extracts Against the Stored Grain Insect Pest Sitophilus granarius L. Arab Journal of Plant Protection, 39(4): 323-328. https://doi.org/10.22268/AJPP-039.4.323328 This study was conducted on six medicinal plants viz., Vitex negundo (leaves), Xanthium strumarium, Caesalpinia bonduc, Mucuna pruriens, Moringa oleifera (seed kernels), Tagetes erecta (petals) for their bio-insecticidal activity. The powders of various parts of plants were extracted using the MARS6 microwave acid digestion system. Three different concentrations (20, 30, and 40%) of plant extracts were tested against granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae) for their bio-insecticidal activities under laboratory conditions. The results of statistical analysis showed a good performance of all plant extracts, especially at the high concentrations of the extracts, where they showed different levels of insect m...
The insecticidal property of dry leaves of Vitex negundo against the Sitophilus granarius in the ... more The insecticidal property of dry leaves of Vitex negundo against the Sitophilus granarius in the laboratory at (28±3) 0 C and (78±3) % r. h. with Soxhlet extracted extracts of V. negundo leaves in ethanol, methanol, chloroform, acetone and n-Hexane were tested and found very effective to control the store grain insect pest. The biopesticidal effects of extracts in different solvents were compared by calculating LD10, LD50, LD90 and LD99 values for 96 hrs. The Probit analysis of data demonstrated that LD values for ethanol, methanol, chloroform, acetone and n-Hexane extracts were LD10-1.216, 1.141, 0.9298, 1.265, 0.5398 ml/Kg; LD50-2.997, 3.106, 2.490, 3.727, 1.302 ml/Kg; LD90-7.392, 8.457, 6.667, 10.98, 3.142 ml/Kg and LD99-15.43, 19.14, 14.88, 26.50, 6.439 ml/Kg respectively, for 96 hrs. These results demonstrated that the mortality increased with increase in concentration of extract and exposure time. There was nearly zero fecundity and next generation was not observed in treated grains. The extracts of V. negundo leaf may be of high value in grain storage against S. granarius, especially in subsistence agriculture where the plants are locally available to local farmers. Hence, we conclude that the ethanol, chloroform and n-Hexane extracts from V. negundo leaf are more effective and useful. It served as being a potential insecticidal agent and could be applicable to the management of populations of stored-product insects particularly against the infestation by granary weevil, S. granarius after proper dose formulation.
The insecticidal property of Datura stramonium seed extracts against the rice weevil, Sitophilus ... more The insecticidal property of Datura stramonium seed extracts against the rice weevil, Sitophilus oryzae was tested in the laboratory. The experiments were conducted at (28±3) 0 C and (78±3) % relative humidity. It was observed that Soxhlet's extracted extracts of D. stramonium seed in ethanol, chloroform and acetone were very effective to control the pest while extracts in methanol and n-hexane gave poor results. The probit analysis of data demonstrated that LD10, LD50 and LD90 values for ethanol, chloroform and acetone extracts was LD10 = 2.962, 3.080, 0.4752 ml/Kg, LD50 = 8.594, 7.379, 1.185 ml/Kg and LD90 = 24.94, 17.67, 2.957 ml/Kg respectively for 96 hrs. These results suggest that the mortality increased with increase in concentration as well as exposure time and the extracts of D. stramonium seed may be of high value in grain storage against S. oryzae, especially in subsistence agriculture where the plants are locally available to farmers with little resources. Hence, we conclude that extracts of D. stramonium seed served as being a potential insecticidal agent can be used against rice weevil, S. oryzae after proper dose formulation to prevent infestation in wheat and even other grains during storage.
Antimicrobial activity of five solvent extracts of local plants was evaluated in vitro, with four... more Antimicrobial activity of five solvent extracts of local plants was evaluated in vitro, with four strains of bacteria viz., Salmonella typhi, Escherichia coli, Shigella flexneri (gram-ve), Staphylococcus aureus (gram +ve) and four strains of fungi viz., Alternaria alternata, Penicillium notatum, Aspergillus niger, Penicillium digitatum microorganism. The in vitro antibacterial and antifungal activities were tested by agar disc diffusion method. The most active antibacterial plants were Vitex negundo, Tagetes erecta and antifungal plants were Xanthium strumarium, Vitex negundo and Tagetes erecta, respectively. The significant antimicrobial activities of potent extracts were compared with the standard antimicrobiotics, Ciprofloxacin and Fluconazole for bacteria as well as fungi respectively at 1 mg/ml concentration. Preliminary phytochemical analysis of V. negundo leaf, X. strumarium, M. pruriens, C. bonduc seed and T. erecta flower extracts generally revealed the presence of Alkaloids, Steroids, Terpenoids, Phenols, Saponins, Anthraquinones, Amino acids, Carotenoids, Flavonoids and Tannins at various concentrations. The results obtained in this study suggest that X. strumarium, V. negundo, T. erecta can be used in treating diseases caused by these test organisms.
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Papers by Dr. Nilesh B Jawalkar