Genetic factors that predispose to hypertension may contribute to element disturbances observed i... more Genetic factors that predispose to hypertension may contribute to element disturbances observed in hyperten-sive patients. We tested the hypothesis that the deletion allele of the angiotensin-converting enzyme (ACE) gene is associated with element imbalances in hypertension. The concentrations of elements in genetically predisposed to hypertension rats (SHRs) and their controls (WKY rats) were also examined. ICP-MS was used for elemental analysis of human hair and animal fur. Genotyping was performed by PCR. We also measured micronuclei frequency and distribution of peripheral blood leukocytes in cell cycle phases by flow cytometry and studied the correlations of these parameters with element contents. In general, the tendency for higher levels of toxic and lower levels of essential elements is observed in hypertension, specifically in patients carrying the D allele. Hypertensive men had significantly higher Be, V, Cr, As, Mo, Ag, Sb, and Na levels and lower Ca, Zn, Ba, and U levels compared with control subjects; the differences were not significant for Mg, Al, K, Mn, Fe, Co, Ni, Cu, Se, Cd, Tl, Pb, and Th. The D allele was associated with higher Be, Mo, and Th levels and lower Zn, Se, and Tl levels. The concentrations of Ca, Co, Mo and U were higher in SHR than those in the WKY rats. Mo, an antagonist of Cu, positively correlated with the S-phase cells, and Cu positively correlated with micronuclei frequency. The results suggest an involvement of the ACE I/D polymorphism in element imbalances in hypertension and attract attention to the possible significant role of genetic factors in Mo accumulation.
Introduction: Increased levels of DNA damage have been observed in individuals with metabolic syn... more Introduction: Increased levels of DNA damage have been observed in individuals with metabolic syndrome (MetS); however, the results were often contradictory. Seasons reportedly have an effect on DNA damage in healthy individuals, but the cell response may differ in patients with MetS, which can contribute to inconsistencies. Aim: The aim of our study was to determine the season-dependent variations in DNA damage levels and to assess the differences between donors with the diseases associated with MetS and healthy individuals. Materials and Methods: Biomarkers of DNA damage, cell death and micronuclei of peripheral blood leukocytes, were measured by flow cytometry in the group of men diagnosed with essential hypertension (n=170), men with diabetes (n=126) and the corresponding control group (n=64). All donors were divided into summer and winter groups according to the time of sampling. Differential white blood cell count was carried out. Results: The patients with the conditions related to MetS had higher levels of cell death and micronuclei compared with the healthy donors (p<0.05). However, taking into account the season of sampling, differences in the levels of DNA damage biomarkers were observed only between the winter groups, because healthy donors had significantly higher cell death rate in summer (p<0.05), while the cells of patients with MetS did not show any response to seasonal change. In winter, micronuclei of patients with MetS correlated with segmented leukocytes (r=0.23, p<0.05), which according to the literature, may signify higher DNA damage in hematopoietic stem cells; healthy donors had a negative correlation between the parameters (r=–0.46, p<0.05). Conclusion: Differences in cellular response to seasonal changes were observed between healthy and donors with MetS factors. The results may be important for using cell death and micronuclei as biomarkers of MetS and for the studies of nature of DNA damage in patients with MetS. Keywords: DNA damage, metabolic syndrome, micronuclei, cell death, season, leukocytes
The aim of this study was to evaluate the association between development of metabolic syndrome a... more The aim of this study was to evaluate the association between development of metabolic syndrome and polymorphisms of oxidative system genes: the markers C1167T of catalase gene CAT (rs769217), T1183C of superoxide dismutase gene SOD2 (rs4880); G172A of superoxide dismutase gene SOD3 (rs2536512). Materials and methods. Genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism method in 81 male patients diagnosed with essential hypertension, type 2 diabetes and with waist circumference ≥ 94 cm. Control group consisted of 64 donors without cardiovascular or metabolic disorders. Biometric and biochemical data were obtained. Results. We found that the allele C of the SOD2 gene is associated with increased risk of metabolic syndrome (OR 2,09, 95 % CI 1,25 – 3,47, p < 0,05). Distribution of SOD2 genotypes were TT – 17,3 %, TC – 55,6 %, CC – 27,2 % and TT – 37,5 %, TC – 48,4 %, CC – 14,1 % in the control group and group of patients with metabolic syndrome, respectively (χ2 = 8,79, p < 0,05). Patients with the TT genotype had lower levels of red blood cells (p < 0,05) and hemoglobin (p > 0,05) when compared with the C allele carriers, which may indicate a lack of oxygen in tissues and cellular response with release of erythropoietin in patients the “unfavorable” C allele. There were no association of SOD3 and CAT gene alleles with metabolic syndrome. Conclusion. The results suggest that the gene polymorphism T1183C SOD2 is an important determining factor in the development of metabolic syndrome and erythrocyte level in male population of Belarus. On further investigation the polymorphism may be used for the development and early adoption of preventive strategies and the formation of groups of patients with a higher risk of complications. Key words: metabolic syndrome, red blood cells, hemoglobin, polymorphism Val16Ala, superoxide dismutase, catalase
—The aim of the study was to evaluate the association between the angiotensin-converting enzyme A... more —The aim of the study was to evaluate the association between the angiotensin-converting enzyme ACE I/D (rs 4340) polymorphism and DNA damage in patients with essential hypertension (EH). The I/D polymorphism of ACE was determined by polymerase chain reaction in 170 male hypertensive patients and 64 normotensive blood donors. We used flow cytometry to determine the levels of cell death, micronuclei and accumulation of peripheral blood leukocytes in G1/G0, S, G2/M phases of the cell cycle. Additionally, the whole blood samples were incubated in vitro at 4°C for 24 h to investigate the genotype effects on the susceptibility of cells to DNA damage. We found lower frequency of cells in DNA synthesis S phase and higher levels of micronuclei in the hypertensive compared to normotensive group (p < 0.05); increased formation of micro-nuclei was seen due to elevated micronuclei frequencies in patients with the ACE II genotype (p < 0.05), but not in ID or DD genotype carriers. Incubation of whole blood samples of normotensive individuals lead to the most active cell death (p < 0.05) and micronuclei formation (p > 0.05) in the II genotype carriers too. However, hypertensive patients displayed different cellular response to incubation-induced DNA damages in the ACE I/D genotype groups; after incubation, the frequencies of micronuclei were significantly higher in the DD genotype carriers (p < 0.05). To conclude, the study suggests that the ACE I/D polymorphism may contribute to mechanisms and intensity of DNA damages in hypertensive and normotensive individuals.
The mean nRBC per 100 WBC level in umbilical blood of newborns in group with prenatal hypoxia was... more The mean nRBC per 100 WBC level in umbilical blood of newborns in group with prenatal hypoxia was 5,67±0,21; whereas that in the control group was 2,21±0,09. This difference was statistically significant (P<0,05). The mean apoptotic WBC level of newborns in group with prenatal hypoxia was 7,09±0,73; whereas that in control group was 2,18 ±1,01 (P<0,05). These markers of cell damage are useful criteria for the rapid assessment of prenatal hypoxia.
. 181 patients with diabetes mellitus (DM) type 2 were involved in the study. They were determine... more . 181 patients with diabetes mellitus (DM) type 2 were involved in the study. They were determined genotype I/D (insertion / deletion) gene polymorphism of angiotensin-converting enzyme (ACE). According to the found out genotypes all participants of the study were divided into 3 groups: II, ID and DD genotypes. The degree of diabetic compensation and risk of neurovascular complications were determine in patients on the level of fasting plasma glucose and HbA1c (recommendation of EASD/ESC). The presence and severity of diabetic peripheral sensorimotor neuropathy was assessed according to TSS, NDS scales and electromyography. It was found out that heterozygous for the I/D polymorphism of ACE gene, more likely to achieve compensated and sub-compensated diabetes in the treatment compared with homozygotes - genotype II and DD. (RR = 2.39, 95% CI 1,44-3,98). The patients with genotype ID had lower risk of neurovascular complications compared with patients with genotype II and DD (RR 0.69, 95% CI 0,52-0,92).
Исследование включило 181 пациента с сахарным диабетом (СД) 2 типа, у которых был определен генот... more Исследование включило 181 пациента с сахарным диабетом (СД) 2 типа, у которых был определен генотип полиморфизма C106T гена альдозоредуктазы и полиморфизма С1167Т гена каталазы. Пациенты обследовались на наличие диабетической периферической сенсомоторной нейропатии с помощью шкал TSS, НДС и электромиографии. Выявлено, что генотип СТ гена альдозоредуктазы ассоциирован с более высокими показателями электромиографии по сенсорным и моторным волокнам. Обнаружена взаимосвязь аллеля С гена альдозоредуктазы с меньшим количеством и интенсивностью жалоб, в то время как у гомозигот с генотипом ТТ интенсивность судорог и онемения выражена сильнее (p<0,05). Ген каталазы в нашем исследовании не был ассоциирован с диабетической сенсомоторной полинейропатией.
Objectives: The aim of this study was to examine the association between methylenetetrahydrofolat... more Objectives: The aim of this study was to examine the association between methylenetetrahydrofolate reductase (MTHFR) C677t polymorphism and leukocyte death, cell-cycle phase distribution and abdominal obesity (AO) in hypertensive patients. Methodology: The study population consisted of 170 males enrolled at a local hospital and diagnosed with essential hypertension (EH). The C677t polymorphism was detected by PCR-RFLP using Hinf I enzyme. We used flow cytometry with propidium iodide staining to distinguish cells in G1/G0, S, G2/M phases of the cell cycle and evaluate cell death in peripheral blood leukocytes. AO was defined by waist circumference (men>94 cm). Results: The genotype distribution was 53.5%, 35.9%, 10.6% for genotypes CC, CT, and TT, respectively (mean age 47.9±8.15). The C677t polymorphism was significantly associated with BMI (p=0.037), family history of EH (p=0.031) and serum creatinine concentrations (p=0.026) in hypertensive patients. Among subjects with AO this polymorphism was significantly associated with the leukocyte death (p=0.043) with the T allele being related to the lower level of cell death. We observed no significant differences in cell-cycle phases when grouped by MTHFR genotype (Table 1). Conclusions: These results suggest that MTHFR C677t polymorphism is associated with leukocyte death when accompanied with abdominal obesity and EH.
The aim of our study is to determine the level of cell death in peripheral blood and the number o... more The aim of our study is to determine the level of cell death in peripheral blood and the number of cells with micronuclei by means of flow cytometry in patients suffering from type 2 diabetes mellitus (T2DM) with the presence or absence of distal polyneuropathy. 176 patients with T2DM and 67 healthy volunteers were examined. Apoptosis of peripheral blood leukocytes and the number of cells with micronuclei increase with longer duration of T2DM, regardless of compensation, patient’s age and the presence of distal sensorimotor polyneuropathy. Determination of apoptosis and the presence of micronucleus test of peripheral blood leukocytes may be the integral marker of pathological changes in T2DM patients. Key words: diabetes mellitus type 2, apoptosis, micronucleus test
This study aims to explore the probable relation between angiotensin converting enzyme (ACE I/D) ... more This study aims to explore the probable relation between angiotensin converting enzyme (ACE I/D) or methylenetetrahydrofolate reductase (MTHFR 677C>T) alleles and proliferation, cell death and micronuclei of leukocytes in hypertensive patients. We genotyped and determined the parameters in 89 individuals with stage II or III essential hypertension. Combination of genotypes I/D + D/D leads to the increased level of cells in G2/M phase of cell cycle compared to I/I genotype. The frequency of cell death was found to be higher in C/C patients compared with the group of C/T genotype and compared with genotypes C/T + T/T (p<0,05). Combined effect of ACE and MTHFR genes on G1/G0 phase of cell cycle was noted.
Molecular and hematologic characteristics of peripheral blood in SHR and WKY under simultaneous e... more Molecular and hematologic characteristics of peripheral blood in SHR and WKY under simultaneous emotional and physical stress were studied. Also, we assessed seasonal variations of leucocyte proliferation and apoptosis in rats and influence on these parameters of the whole blood incubation at 4ºС for 24 hours. In order to assess characteristics of peripheral blood we used hematology analyzer, leucocyte molecular and biological parameters were evaluated by a flow cytometer. Negative influence of the intense prolonged exercises on the hematologic characteristics of the animal blood was noted. There was a significant fall of the number of immune cells in peripheral blood of the rats and intensification of leucocyte apoptosis, particularly in SHR. The parameters varied significantly during the year indicating more flexible reaction of WKY on seasonal changes. Light microscopy showed that incubation of the whole blood made rat leucocytes considerably liable to apoptotic changes.
Intense prolonged treadmill exercises cause lymphocytopenia that is displayed as significant fall... more Intense prolonged treadmill exercises cause lymphocytopenia that is displayed as significant fall of the amount of immune cells in peripheral blood of hypertensive SHR and their normotensive control WKY rats. Suppression of immune system is especially strong in SHR comparing to WKY. The effect on the amount of immune cells is significantly weaker when the complex of vitamins and minerals UNIVIT is added to the usual ration of SHR. Furthermore, UNIVIT enhances proliferation among leukocytes in peripheral blood of SHR that results in smaller percentage of cells in G1/G0 phase and increased percentage of leukocytes in S phase of cell cycle. Thus, complex of vitamins and minerals UNIVIT improves adaptive qualities of an organism under condition of spontaneous or induced immunodeficiency in SHR.
Genetic factors that predispose to hypertension may contribute to element disturbances observed i... more Genetic factors that predispose to hypertension may contribute to element disturbances observed in hyperten-sive patients. We tested the hypothesis that the deletion allele of the angiotensin-converting enzyme (ACE) gene is associated with element imbalances in hypertension. The concentrations of elements in genetically predisposed to hypertension rats (SHRs) and their controls (WKY rats) were also examined. ICP-MS was used for elemental analysis of human hair and animal fur. Genotyping was performed by PCR. We also measured micronuclei frequency and distribution of peripheral blood leukocytes in cell cycle phases by flow cytometry and studied the correlations of these parameters with element contents. In general, the tendency for higher levels of toxic and lower levels of essential elements is observed in hypertension, specifically in patients carrying the D allele. Hypertensive men had significantly higher Be, V, Cr, As, Mo, Ag, Sb, and Na levels and lower Ca, Zn, Ba, and U levels compared with control subjects; the differences were not significant for Mg, Al, K, Mn, Fe, Co, Ni, Cu, Se, Cd, Tl, Pb, and Th. The D allele was associated with higher Be, Mo, and Th levels and lower Zn, Se, and Tl levels. The concentrations of Ca, Co, Mo and U were higher in SHR than those in the WKY rats. Mo, an antagonist of Cu, positively correlated with the S-phase cells, and Cu positively correlated with micronuclei frequency. The results suggest an involvement of the ACE I/D polymorphism in element imbalances in hypertension and attract attention to the possible significant role of genetic factors in Mo accumulation.
Introduction: Increased levels of DNA damage have been observed in individuals with metabolic syn... more Introduction: Increased levels of DNA damage have been observed in individuals with metabolic syndrome (MetS); however, the results were often contradictory. Seasons reportedly have an effect on DNA damage in healthy individuals, but the cell response may differ in patients with MetS, which can contribute to inconsistencies. Aim: The aim of our study was to determine the season-dependent variations in DNA damage levels and to assess the differences between donors with the diseases associated with MetS and healthy individuals. Materials and Methods: Biomarkers of DNA damage, cell death and micronuclei of peripheral blood leukocytes, were measured by flow cytometry in the group of men diagnosed with essential hypertension (n=170), men with diabetes (n=126) and the corresponding control group (n=64). All donors were divided into summer and winter groups according to the time of sampling. Differential white blood cell count was carried out. Results: The patients with the conditions related to MetS had higher levels of cell death and micronuclei compared with the healthy donors (p<0.05). However, taking into account the season of sampling, differences in the levels of DNA damage biomarkers were observed only between the winter groups, because healthy donors had significantly higher cell death rate in summer (p<0.05), while the cells of patients with MetS did not show any response to seasonal change. In winter, micronuclei of patients with MetS correlated with segmented leukocytes (r=0.23, p<0.05), which according to the literature, may signify higher DNA damage in hematopoietic stem cells; healthy donors had a negative correlation between the parameters (r=–0.46, p<0.05). Conclusion: Differences in cellular response to seasonal changes were observed between healthy and donors with MetS factors. The results may be important for using cell death and micronuclei as biomarkers of MetS and for the studies of nature of DNA damage in patients with MetS. Keywords: DNA damage, metabolic syndrome, micronuclei, cell death, season, leukocytes
The aim of this study was to evaluate the association between development of metabolic syndrome a... more The aim of this study was to evaluate the association between development of metabolic syndrome and polymorphisms of oxidative system genes: the markers C1167T of catalase gene CAT (rs769217), T1183C of superoxide dismutase gene SOD2 (rs4880); G172A of superoxide dismutase gene SOD3 (rs2536512). Materials and methods. Genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism method in 81 male patients diagnosed with essential hypertension, type 2 diabetes and with waist circumference ≥ 94 cm. Control group consisted of 64 donors without cardiovascular or metabolic disorders. Biometric and biochemical data were obtained. Results. We found that the allele C of the SOD2 gene is associated with increased risk of metabolic syndrome (OR 2,09, 95 % CI 1,25 – 3,47, p < 0,05). Distribution of SOD2 genotypes were TT – 17,3 %, TC – 55,6 %, CC – 27,2 % and TT – 37,5 %, TC – 48,4 %, CC – 14,1 % in the control group and group of patients with metabolic syndrome, respectively (χ2 = 8,79, p < 0,05). Patients with the TT genotype had lower levels of red blood cells (p < 0,05) and hemoglobin (p > 0,05) when compared with the C allele carriers, which may indicate a lack of oxygen in tissues and cellular response with release of erythropoietin in patients the “unfavorable” C allele. There were no association of SOD3 and CAT gene alleles with metabolic syndrome. Conclusion. The results suggest that the gene polymorphism T1183C SOD2 is an important determining factor in the development of metabolic syndrome and erythrocyte level in male population of Belarus. On further investigation the polymorphism may be used for the development and early adoption of preventive strategies and the formation of groups of patients with a higher risk of complications. Key words: metabolic syndrome, red blood cells, hemoglobin, polymorphism Val16Ala, superoxide dismutase, catalase
—The aim of the study was to evaluate the association between the angiotensin-converting enzyme A... more —The aim of the study was to evaluate the association between the angiotensin-converting enzyme ACE I/D (rs 4340) polymorphism and DNA damage in patients with essential hypertension (EH). The I/D polymorphism of ACE was determined by polymerase chain reaction in 170 male hypertensive patients and 64 normotensive blood donors. We used flow cytometry to determine the levels of cell death, micronuclei and accumulation of peripheral blood leukocytes in G1/G0, S, G2/M phases of the cell cycle. Additionally, the whole blood samples were incubated in vitro at 4°C for 24 h to investigate the genotype effects on the susceptibility of cells to DNA damage. We found lower frequency of cells in DNA synthesis S phase and higher levels of micronuclei in the hypertensive compared to normotensive group (p < 0.05); increased formation of micro-nuclei was seen due to elevated micronuclei frequencies in patients with the ACE II genotype (p < 0.05), but not in ID or DD genotype carriers. Incubation of whole blood samples of normotensive individuals lead to the most active cell death (p < 0.05) and micronuclei formation (p > 0.05) in the II genotype carriers too. However, hypertensive patients displayed different cellular response to incubation-induced DNA damages in the ACE I/D genotype groups; after incubation, the frequencies of micronuclei were significantly higher in the DD genotype carriers (p < 0.05). To conclude, the study suggests that the ACE I/D polymorphism may contribute to mechanisms and intensity of DNA damages in hypertensive and normotensive individuals.
The mean nRBC per 100 WBC level in umbilical blood of newborns in group with prenatal hypoxia was... more The mean nRBC per 100 WBC level in umbilical blood of newborns in group with prenatal hypoxia was 5,67±0,21; whereas that in the control group was 2,21±0,09. This difference was statistically significant (P<0,05). The mean apoptotic WBC level of newborns in group with prenatal hypoxia was 7,09±0,73; whereas that in control group was 2,18 ±1,01 (P<0,05). These markers of cell damage are useful criteria for the rapid assessment of prenatal hypoxia.
. 181 patients with diabetes mellitus (DM) type 2 were involved in the study. They were determine... more . 181 patients with diabetes mellitus (DM) type 2 were involved in the study. They were determined genotype I/D (insertion / deletion) gene polymorphism of angiotensin-converting enzyme (ACE). According to the found out genotypes all participants of the study were divided into 3 groups: II, ID and DD genotypes. The degree of diabetic compensation and risk of neurovascular complications were determine in patients on the level of fasting plasma glucose and HbA1c (recommendation of EASD/ESC). The presence and severity of diabetic peripheral sensorimotor neuropathy was assessed according to TSS, NDS scales and electromyography. It was found out that heterozygous for the I/D polymorphism of ACE gene, more likely to achieve compensated and sub-compensated diabetes in the treatment compared with homozygotes - genotype II and DD. (RR = 2.39, 95% CI 1,44-3,98). The patients with genotype ID had lower risk of neurovascular complications compared with patients with genotype II and DD (RR 0.69, 95% CI 0,52-0,92).
Исследование включило 181 пациента с сахарным диабетом (СД) 2 типа, у которых был определен генот... more Исследование включило 181 пациента с сахарным диабетом (СД) 2 типа, у которых был определен генотип полиморфизма C106T гена альдозоредуктазы и полиморфизма С1167Т гена каталазы. Пациенты обследовались на наличие диабетической периферической сенсомоторной нейропатии с помощью шкал TSS, НДС и электромиографии. Выявлено, что генотип СТ гена альдозоредуктазы ассоциирован с более высокими показателями электромиографии по сенсорным и моторным волокнам. Обнаружена взаимосвязь аллеля С гена альдозоредуктазы с меньшим количеством и интенсивностью жалоб, в то время как у гомозигот с генотипом ТТ интенсивность судорог и онемения выражена сильнее (p<0,05). Ген каталазы в нашем исследовании не был ассоциирован с диабетической сенсомоторной полинейропатией.
Objectives: The aim of this study was to examine the association between methylenetetrahydrofolat... more Objectives: The aim of this study was to examine the association between methylenetetrahydrofolate reductase (MTHFR) C677t polymorphism and leukocyte death, cell-cycle phase distribution and abdominal obesity (AO) in hypertensive patients. Methodology: The study population consisted of 170 males enrolled at a local hospital and diagnosed with essential hypertension (EH). The C677t polymorphism was detected by PCR-RFLP using Hinf I enzyme. We used flow cytometry with propidium iodide staining to distinguish cells in G1/G0, S, G2/M phases of the cell cycle and evaluate cell death in peripheral blood leukocytes. AO was defined by waist circumference (men>94 cm). Results: The genotype distribution was 53.5%, 35.9%, 10.6% for genotypes CC, CT, and TT, respectively (mean age 47.9±8.15). The C677t polymorphism was significantly associated with BMI (p=0.037), family history of EH (p=0.031) and serum creatinine concentrations (p=0.026) in hypertensive patients. Among subjects with AO this polymorphism was significantly associated with the leukocyte death (p=0.043) with the T allele being related to the lower level of cell death. We observed no significant differences in cell-cycle phases when grouped by MTHFR genotype (Table 1). Conclusions: These results suggest that MTHFR C677t polymorphism is associated with leukocyte death when accompanied with abdominal obesity and EH.
The aim of our study is to determine the level of cell death in peripheral blood and the number o... more The aim of our study is to determine the level of cell death in peripheral blood and the number of cells with micronuclei by means of flow cytometry in patients suffering from type 2 diabetes mellitus (T2DM) with the presence or absence of distal polyneuropathy. 176 patients with T2DM and 67 healthy volunteers were examined. Apoptosis of peripheral blood leukocytes and the number of cells with micronuclei increase with longer duration of T2DM, regardless of compensation, patient’s age and the presence of distal sensorimotor polyneuropathy. Determination of apoptosis and the presence of micronucleus test of peripheral blood leukocytes may be the integral marker of pathological changes in T2DM patients. Key words: diabetes mellitus type 2, apoptosis, micronucleus test
This study aims to explore the probable relation between angiotensin converting enzyme (ACE I/D) ... more This study aims to explore the probable relation between angiotensin converting enzyme (ACE I/D) or methylenetetrahydrofolate reductase (MTHFR 677C>T) alleles and proliferation, cell death and micronuclei of leukocytes in hypertensive patients. We genotyped and determined the parameters in 89 individuals with stage II or III essential hypertension. Combination of genotypes I/D + D/D leads to the increased level of cells in G2/M phase of cell cycle compared to I/I genotype. The frequency of cell death was found to be higher in C/C patients compared with the group of C/T genotype and compared with genotypes C/T + T/T (p<0,05). Combined effect of ACE and MTHFR genes on G1/G0 phase of cell cycle was noted.
Molecular and hematologic characteristics of peripheral blood in SHR and WKY under simultaneous e... more Molecular and hematologic characteristics of peripheral blood in SHR and WKY under simultaneous emotional and physical stress were studied. Also, we assessed seasonal variations of leucocyte proliferation and apoptosis in rats and influence on these parameters of the whole blood incubation at 4ºС for 24 hours. In order to assess characteristics of peripheral blood we used hematology analyzer, leucocyte molecular and biological parameters were evaluated by a flow cytometer. Negative influence of the intense prolonged exercises on the hematologic characteristics of the animal blood was noted. There was a significant fall of the number of immune cells in peripheral blood of the rats and intensification of leucocyte apoptosis, particularly in SHR. The parameters varied significantly during the year indicating more flexible reaction of WKY on seasonal changes. Light microscopy showed that incubation of the whole blood made rat leucocytes considerably liable to apoptotic changes.
Intense prolonged treadmill exercises cause lymphocytopenia that is displayed as significant fall... more Intense prolonged treadmill exercises cause lymphocytopenia that is displayed as significant fall of the amount of immune cells in peripheral blood of hypertensive SHR and their normotensive control WKY rats. Suppression of immune system is especially strong in SHR comparing to WKY. The effect on the amount of immune cells is significantly weaker when the complex of vitamins and minerals UNIVIT is added to the usual ration of SHR. Furthermore, UNIVIT enhances proliferation among leukocytes in peripheral blood of SHR that results in smaller percentage of cells in G1/G0 phase and increased percentage of leukocytes in S phase of cell cycle. Thus, complex of vitamins and minerals UNIVIT improves adaptive qualities of an organism under condition of spontaneous or induced immunodeficiency in SHR.
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Papers by Olena Pavlyushchik
Aim: The aim of our study was to determine the season-dependent variations in DNA damage levels and to assess the differences between donors with the diseases associated with MetS and healthy individuals.
Materials and Methods: Biomarkers of DNA damage, cell death and micronuclei of peripheral blood leukocytes, were measured by flow cytometry in the group of men diagnosed with essential hypertension (n=170), men with diabetes (n=126) and the corresponding control group (n=64). All donors were divided into summer and winter groups according to the time of sampling. Differential white blood cell count was carried out.
Results: The patients with the conditions related to MetS had higher levels of cell death and micronuclei compared with the healthy donors (p<0.05). However, taking into account the season of sampling, differences in the levels of DNA damage biomarkers were observed only between the winter groups, because healthy donors had significantly higher cell death rate in summer (p<0.05), while the cells of patients with MetS did not show any response to seasonal change. In winter, micronuclei of patients with MetS correlated with segmented leukocytes (r=0.23, p<0.05), which according to the literature, may signify higher DNA damage in hematopoietic stem cells; healthy donors had a negative correlation between the parameters (r=–0.46, p<0.05).
Conclusion: Differences in cellular response to seasonal changes were observed between healthy and donors with MetS factors. The results may be important for using cell death and micronuclei as biomarkers of MetS and for the studies of nature of DNA damage in patients with MetS.
Keywords: DNA damage, metabolic syndrome, micronuclei, cell death, season, leukocytes
Materials and methods. Genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism method in 81 male patients diagnosed with essential hypertension, type 2 diabetes and with waist circumference ≥ 94 cm. Control group consisted of 64 donors without cardiovascular or metabolic disorders. Biometric and biochemical data were obtained.
Results. We found that the allele C of the SOD2 gene is associated with increased risk of metabolic syndrome (OR 2,09, 95 % CI 1,25 – 3,47, p < 0,05). Distribution of SOD2 genotypes were TT – 17,3 %, TC – 55,6 %, CC – 27,2 % and TT – 37,5 %, TC – 48,4 %, CC – 14,1 % in the control group and group of patients with metabolic syndrome, respectively (χ2 = 8,79, p < 0,05). Patients with the TT genotype had lower levels of red blood cells (p < 0,05) and hemoglobin (p > 0,05) when compared with the C allele carriers, which may indicate a lack of oxygen in tissues and cellular response with release of erythropoietin in patients the “unfavorable” C allele. There were no association of SOD3 and CAT gene alleles with metabolic syndrome.
Conclusion. The results suggest that the gene polymorphism T1183C SOD2 is an important determining factor in the development of metabolic syndrome and erythrocyte level in male population of Belarus. On further investigation the polymorphism may be used for the development and early adoption of preventive strategies and the formation of groups of patients with a higher risk of complications.
Key words: metabolic syndrome, red blood cells, hemoglobin, polymorphism Val16Ala, superoxide dismutase, catalase
Methodology: The study population consisted of 170 males enrolled at a local hospital and diagnosed with essential hypertension (EH). The C677t polymorphism was detected by PCR-RFLP using Hinf I enzyme. We used flow cytometry with propidium iodide staining to distinguish cells in G1/G0, S, G2/M phases of the cell cycle and evaluate cell death in peripheral blood leukocytes. AO was defined by waist circumference (men>94 cm).
Results: The genotype distribution was 53.5%, 35.9%, 10.6% for genotypes CC, CT, and TT, respectively (mean age 47.9±8.15). The C677t polymorphism was significantly associated with BMI (p=0.037), family history of EH (p=0.031) and serum creatinine concentrations (p=0.026) in hypertensive patients. Among subjects with AO this polymorphism was significantly associated with the leukocyte death (p=0.043) with the T allele being related to the lower level of cell death. We observed no significant differences in cell-cycle phases when grouped by MTHFR genotype (Table 1).
Conclusions: These results suggest that MTHFR C677t polymorphism is associated with leukocyte death when accompanied with abdominal obesity and EH.
by means of flow cytometry in patients suffering from type 2 diabetes mellitus (T2DM) with the presence or absence of distal
polyneuropathy. 176 patients with T2DM and 67 healthy volunteers were examined. Apoptosis of peripheral blood leukocytes
and the number of cells with micronuclei increase with longer duration of T2DM, regardless of compensation, patient’s age
and the presence of distal sensorimotor polyneuropathy. Determination of apoptosis and the presence of micronucleus test
of peripheral blood leukocytes may be the integral marker of pathological changes in T2DM patients.
Key words: diabetes mellitus type 2, apoptosis, micronucleus test
Aim: The aim of our study was to determine the season-dependent variations in DNA damage levels and to assess the differences between donors with the diseases associated with MetS and healthy individuals.
Materials and Methods: Biomarkers of DNA damage, cell death and micronuclei of peripheral blood leukocytes, were measured by flow cytometry in the group of men diagnosed with essential hypertension (n=170), men with diabetes (n=126) and the corresponding control group (n=64). All donors were divided into summer and winter groups according to the time of sampling. Differential white blood cell count was carried out.
Results: The patients with the conditions related to MetS had higher levels of cell death and micronuclei compared with the healthy donors (p<0.05). However, taking into account the season of sampling, differences in the levels of DNA damage biomarkers were observed only between the winter groups, because healthy donors had significantly higher cell death rate in summer (p<0.05), while the cells of patients with MetS did not show any response to seasonal change. In winter, micronuclei of patients with MetS correlated with segmented leukocytes (r=0.23, p<0.05), which according to the literature, may signify higher DNA damage in hematopoietic stem cells; healthy donors had a negative correlation between the parameters (r=–0.46, p<0.05).
Conclusion: Differences in cellular response to seasonal changes were observed between healthy and donors with MetS factors. The results may be important for using cell death and micronuclei as biomarkers of MetS and for the studies of nature of DNA damage in patients with MetS.
Keywords: DNA damage, metabolic syndrome, micronuclei, cell death, season, leukocytes
Materials and methods. Genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism method in 81 male patients diagnosed with essential hypertension, type 2 diabetes and with waist circumference ≥ 94 cm. Control group consisted of 64 donors without cardiovascular or metabolic disorders. Biometric and biochemical data were obtained.
Results. We found that the allele C of the SOD2 gene is associated with increased risk of metabolic syndrome (OR 2,09, 95 % CI 1,25 – 3,47, p < 0,05). Distribution of SOD2 genotypes were TT – 17,3 %, TC – 55,6 %, CC – 27,2 % and TT – 37,5 %, TC – 48,4 %, CC – 14,1 % in the control group and group of patients with metabolic syndrome, respectively (χ2 = 8,79, p < 0,05). Patients with the TT genotype had lower levels of red blood cells (p < 0,05) and hemoglobin (p > 0,05) when compared with the C allele carriers, which may indicate a lack of oxygen in tissues and cellular response with release of erythropoietin in patients the “unfavorable” C allele. There were no association of SOD3 and CAT gene alleles with metabolic syndrome.
Conclusion. The results suggest that the gene polymorphism T1183C SOD2 is an important determining factor in the development of metabolic syndrome and erythrocyte level in male population of Belarus. On further investigation the polymorphism may be used for the development and early adoption of preventive strategies and the formation of groups of patients with a higher risk of complications.
Key words: metabolic syndrome, red blood cells, hemoglobin, polymorphism Val16Ala, superoxide dismutase, catalase
Methodology: The study population consisted of 170 males enrolled at a local hospital and diagnosed with essential hypertension (EH). The C677t polymorphism was detected by PCR-RFLP using Hinf I enzyme. We used flow cytometry with propidium iodide staining to distinguish cells in G1/G0, S, G2/M phases of the cell cycle and evaluate cell death in peripheral blood leukocytes. AO was defined by waist circumference (men>94 cm).
Results: The genotype distribution was 53.5%, 35.9%, 10.6% for genotypes CC, CT, and TT, respectively (mean age 47.9±8.15). The C677t polymorphism was significantly associated with BMI (p=0.037), family history of EH (p=0.031) and serum creatinine concentrations (p=0.026) in hypertensive patients. Among subjects with AO this polymorphism was significantly associated with the leukocyte death (p=0.043) with the T allele being related to the lower level of cell death. We observed no significant differences in cell-cycle phases when grouped by MTHFR genotype (Table 1).
Conclusions: These results suggest that MTHFR C677t polymorphism is associated with leukocyte death when accompanied with abdominal obesity and EH.
by means of flow cytometry in patients suffering from type 2 diabetes mellitus (T2DM) with the presence or absence of distal
polyneuropathy. 176 patients with T2DM and 67 healthy volunteers were examined. Apoptosis of peripheral blood leukocytes
and the number of cells with micronuclei increase with longer duration of T2DM, regardless of compensation, patient’s age
and the presence of distal sensorimotor polyneuropathy. Determination of apoptosis and the presence of micronucleus test
of peripheral blood leukocytes may be the integral marker of pathological changes in T2DM patients.
Key words: diabetes mellitus type 2, apoptosis, micronucleus test