Biodegradation is now being considered as the best form of cyanide transformation because it is l... more Biodegradation is now being considered as the best form of cyanide transformation because it is less expensive and has fewer hazards than other conventional methods. Soil and drainage water samples from the premises of Univerisiti Putra Malaysia (UPM) Serdang, were collected at different points and analysed for available bacteria. 28 bacteria were isolated from the samples and subjected to in-vitro cyanide remediation assay by using spectrophotometric method. Six bacterial isolates were observed to have cyanide-degrading ability. Screening was conducted in three phases that include primary, secondary and tertiary screening. In the primary phase of the screening, the six bacterial isolates were assayed for cyanide-degrading activity using 25 ppm filter-sterilised KCN in a buffer medium (pH 7.0) containing 5 g/L glucose, and 0.5% yeast extract. It was observed that three isolates have a greater potential of converting cyanide, degrading above 70%. The three isolates were further subjected to screening which is the secondary screening by incorporating 100 ppm of filter sterilised KCN. All three isolates showed good ability of degradation removing above 60% of the cyanide. The isolate tagged as AQ-007 was found to have a better degradation capability, removing 75% of the cyanide while isolate AQ-003 and AQ-004 degraded 61.5% and 64.6% respectively. The tertiary screening phase was carried out using resting cells of the bacteria. The resting cells illustrates that it is the best form of inoculums. Isolates AQ-003 removed 92.8%, AQ-004 remediated 92.9% and AQ- 007 degraded 98% of the cyanide. Isolate AQ-007 was considered to be the preferred bacteria for further research because it degrades higher than the other two isolates. Bacterial identification based on 16s rRNA gene sequence analysis of this bacteria showed a 97% identity with Serratia marcescens. It has been registered in the GenBank as Serratia marcescens Strain AQ07 with accession number KP213291
Journal of Environmental Microbiology and Toxicology
Recently, molybdenum is considered as an emerging pollutant for its extreme toxicity to spermatog... more Recently, molybdenum is considered as an emerging pollutant for its extreme toxicity to spermatogenesis in some organisms. Bacterial molybdate reduction to colloidal molybdenum blue (Mo-blue) forms the basis for its bioremediation. Molybdenum-reducing Pseudomonas aeruginosa strain KIK-11 was screened for its potential to degrade hydrocarbons and detergents. Optimal molybdate reduction to Mo-blue in this strain was supported by pH between 5.8 and 6.0, temperatures between 25 and 34 oC, molybdate concentration between 30 and 40 mM and a critical phosphate concentration of between 5.0 and 7.5 mM. The isolate was able to survive and grow on SDS and diesel. However, these compounds did not support Mo-blue production. The best electron donor source facilitating molybdate reduction is glucose, followed by galactose, fructose and citrate respectively. The process was inhibited by heavy metals such as copper (II), mercury (II) and silver (I). The bacterium was able to grow and detoxify multi...
Journal of Advances in Biology & Biotechnology
Background of Study: Rumen microorganisms are involved in the fermentation of substrates containe... more Background of Study: Rumen microorganisms are involved in the fermentation of substrates contained in the diet of the animals. Research on the isolation and identification of cellulase enzyme-producing bacteria is still widespread. Biodegradation by cellulolytic bacteria found in rumen can be used as a source of cellulolytic bacteria which will function to degrade fibrous feed material so as to increase quality of nutrition and digestibility of ingredients at a cheaper price than the use of commercial cellulase production. Aim: To isolate and characterize bacteria from animals’ rumen for their ability to hydrolyze cellulose. Place and Duration of Study: Conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi state, Nigeria, between April to July, 2021. Methods: Rumen of cows and goats was sliced and randomly swabbed with a swab stick. They were then inoculated on Nutrient and MacConkey agar media. The plates were then incubated aerobically and an...
Journal of Environmental Microbiology and Toxicology, 2014
Effect of heavy metals was tested on Serratia marcescens strain AQ07 (accession number: KP213291)... more Effect of heavy metals was tested on Serratia marcescens strain AQ07 (accession number: KP213291) to affirm its effect on growth and biodegradation of cyanide. 1 ppm of ten different heavy metals was experimented in-vitro in buffer medium containing 200 ppm potassium cyanide. They are: silver, arsenic, cadmium, cobalt, chromium, cupper, mercury, nickel, lead and zinc. The results obtained illustrates that mercury have significant effect on the growth (optical density 0.13) and biodegradation of cyanide removing only 24.7% compared to the control which has no incorporation of heavy metal (optical density 0.74 and 92% removal respectively) while others remove above 80%. Further studies on mercury reveal that it has significant effect on the bacteria even as low as 0.1 ppm. This illustrates that mercury can significantly hinder biodegradation of cyanide by Serratia marcescens strain AQ07.
Journal of Biochemistry, Microbiology and Biotechnology, 2019
The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as deterge... more The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as detergent in industrial washing, which results in the high effluent level of this contaminant. SDS as anionic surfactant is ubiquitously toxic to the aquatic ecosystem. In this study, the potentials of a previously isolated molybdenum-reducing Bacillus amyloliquefaciens strain KIK-12 to degrade and utilize SDS as sole source of carbon was investigated. The bacterium grew optimally at pH between 6.0 and 7.0, temperature between 30 and 45 °C in 1 g/L SDS as the sole source of carbon, with ammonium sulphate (1 % w/v) as the best nitrogen source. The growth characteristics of strain KIK-12 on various concentrations of SDS (as a carbon source) reveals optimum growth 500 mg/L but was able to tolerate and grow at 1500 mg/L. However, concentrations higher than this results in growth termination. Heavy metals such as mercury, silver and copper significantly inhibit growth of strain KIK-12 on SDS. The a...
The treatment of parasitic diseases is multifaceted. The control methods require a complex interp... more The treatment of parasitic diseases is multifaceted. The control methods require a complex interplay involving experts in public health, government policies, education, and medical sciences. Several strategies used in the treatment of parasitic diseases are considered and they are based on the availability, effectiveness, affordability, and acceptability of the used drug. Other measures include effective elimination of vector, and animal reservoirs. Interestingly, new strategies and approaches for the treatment of parasitic diseases involve nanomedical encapsulation of drugs and active compounds. Furthermore, genome, cells, and signal pathways targeting have been used for preventing and treating parasitic diseases. These approaches are used for diagnosis, and treatments of disease and to gain increased understanding of underlying disease mechanisms. Phytocompounds such as flavonoids and others are used in nanotherapeutics for treating parasitic diseases as they prevent oxidation of ...
Biodegradation is now being considered as the best form of cyanide transformation because it is l... more Biodegradation is now being considered as the best form of cyanide transformation because it is less expensive and has fewer hazards than other conventional methods. Soil and drainage water samples from the premises of Univerisiti Putra Malaysia (UPM) Serdang, were collected at different points and analysed for available bacteria. 28 bacteria were isolated from the samples and subjected to in-vitro cyanide remediation assay by using spectrophotometric method. Six bacterial isolates were observed to have cyanide-degrading ability. Screening was conducted in three phases that include primary, secondary and tertiary screening. In the primary phase of the screening, the six bacterial isolates were assayed for cyanide-degrading activity using 25 ppm filter-sterilised KCN in a buffer medium (pH 7.0) containing 5 g/L glucose, and 0.5% yeast extract. It was observed that three isolates have a greater potential of converting cyanide, degrading above 70%. The three isolates were further subje...
Biodegradation is now being considered as the best form of cyanide transformation because it is l... more Biodegradation is now being considered as the best form of cyanide transformation because it is less expensive and has fewer hazards than other conventional methods. Soil and drainage water samples from the premises of Univerisiti Putra Malaysia (UPM) Serdang, were collected at different points and analysed for available bacteria. 28 bacteria were isolated from the samples and subjected to in-vitro cyanide remediation assay by using spectrophotometric method. Six bacterial isolates were observed to have cyanide-degrading ability. Screening was conducted in three phases that include primary, secondary and tertiary screening. In the primary phase of the screening, the six bacterial isolates were assayed for cyanide-degrading activity using 25 ppm filter-sterilised KCN in a buffer medium (pH 7.0) containing 5 g/L glucose, and 0.5% yeast extract. It was observed that three isolates have a greater potential of converting cyanide, degrading above 70%. The three isolates were further subjected to screening which is the secondary screening by incorporating 100 ppm of filter sterilised KCN. All three isolates showed good ability of degradation removing above 60% of the cyanide. The isolate tagged as AQ-007 was found to have a better degradation capability, removing 75% of the cyanide while isolate AQ-003 and AQ-004 degraded 61.5% and 64.6% respectively. The tertiary screening phase was carried out using resting cells of the bacteria. The resting cells illustrates that it is the best form of inoculums. Isolates AQ-003 removed 92.8%, AQ-004 remediated 92.9% and AQ- 007 degraded 98% of the cyanide. Isolate AQ-007 was considered to be the preferred bacteria for further research because it degrades higher than the other two isolates. Bacterial identification based on 16s rRNA gene sequence analysis of this bacteria showed a 97% identity with Serratia marcescens. It has been registered in the GenBank as Serratia marcescens Strain AQ07 with accession number KP213291
Journal of Environmental Microbiology and Toxicology
Recently, molybdenum is considered as an emerging pollutant for its extreme toxicity to spermatog... more Recently, molybdenum is considered as an emerging pollutant for its extreme toxicity to spermatogenesis in some organisms. Bacterial molybdate reduction to colloidal molybdenum blue (Mo-blue) forms the basis for its bioremediation. Molybdenum-reducing Pseudomonas aeruginosa strain KIK-11 was screened for its potential to degrade hydrocarbons and detergents. Optimal molybdate reduction to Mo-blue in this strain was supported by pH between 5.8 and 6.0, temperatures between 25 and 34 oC, molybdate concentration between 30 and 40 mM and a critical phosphate concentration of between 5.0 and 7.5 mM. The isolate was able to survive and grow on SDS and diesel. However, these compounds did not support Mo-blue production. The best electron donor source facilitating molybdate reduction is glucose, followed by galactose, fructose and citrate respectively. The process was inhibited by heavy metals such as copper (II), mercury (II) and silver (I). The bacterium was able to grow and detoxify multi...
Journal of Advances in Biology & Biotechnology
Background of Study: Rumen microorganisms are involved in the fermentation of substrates containe... more Background of Study: Rumen microorganisms are involved in the fermentation of substrates contained in the diet of the animals. Research on the isolation and identification of cellulase enzyme-producing bacteria is still widespread. Biodegradation by cellulolytic bacteria found in rumen can be used as a source of cellulolytic bacteria which will function to degrade fibrous feed material so as to increase quality of nutrition and digestibility of ingredients at a cheaper price than the use of commercial cellulase production. Aim: To isolate and characterize bacteria from animals’ rumen for their ability to hydrolyze cellulose. Place and Duration of Study: Conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi state, Nigeria, between April to July, 2021. Methods: Rumen of cows and goats was sliced and randomly swabbed with a swab stick. They were then inoculated on Nutrient and MacConkey agar media. The plates were then incubated aerobically and an...
Journal of Environmental Microbiology and Toxicology, 2014
Effect of heavy metals was tested on Serratia marcescens strain AQ07 (accession number: KP213291)... more Effect of heavy metals was tested on Serratia marcescens strain AQ07 (accession number: KP213291) to affirm its effect on growth and biodegradation of cyanide. 1 ppm of ten different heavy metals was experimented in-vitro in buffer medium containing 200 ppm potassium cyanide. They are: silver, arsenic, cadmium, cobalt, chromium, cupper, mercury, nickel, lead and zinc. The results obtained illustrates that mercury have significant effect on the growth (optical density 0.13) and biodegradation of cyanide removing only 24.7% compared to the control which has no incorporation of heavy metal (optical density 0.74 and 92% removal respectively) while others remove above 80%. Further studies on mercury reveal that it has significant effect on the bacteria even as low as 0.1 ppm. This illustrates that mercury can significantly hinder biodegradation of cyanide by Serratia marcescens strain AQ07.
Journal of Biochemistry, Microbiology and Biotechnology, 2019
The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as deterge... more The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as detergent in industrial washing, which results in the high effluent level of this contaminant. SDS as anionic surfactant is ubiquitously toxic to the aquatic ecosystem. In this study, the potentials of a previously isolated molybdenum-reducing Bacillus amyloliquefaciens strain KIK-12 to degrade and utilize SDS as sole source of carbon was investigated. The bacterium grew optimally at pH between 6.0 and 7.0, temperature between 30 and 45 °C in 1 g/L SDS as the sole source of carbon, with ammonium sulphate (1 % w/v) as the best nitrogen source. The growth characteristics of strain KIK-12 on various concentrations of SDS (as a carbon source) reveals optimum growth 500 mg/L but was able to tolerate and grow at 1500 mg/L. However, concentrations higher than this results in growth termination. Heavy metals such as mercury, silver and copper significantly inhibit growth of strain KIK-12 on SDS. The a...
The treatment of parasitic diseases is multifaceted. The control methods require a complex interp... more The treatment of parasitic diseases is multifaceted. The control methods require a complex interplay involving experts in public health, government policies, education, and medical sciences. Several strategies used in the treatment of parasitic diseases are considered and they are based on the availability, effectiveness, affordability, and acceptability of the used drug. Other measures include effective elimination of vector, and animal reservoirs. Interestingly, new strategies and approaches for the treatment of parasitic diseases involve nanomedical encapsulation of drugs and active compounds. Furthermore, genome, cells, and signal pathways targeting have been used for preventing and treating parasitic diseases. These approaches are used for diagnosis, and treatments of disease and to gain increased understanding of underlying disease mechanisms. Phytocompounds such as flavonoids and others are used in nanotherapeutics for treating parasitic diseases as they prevent oxidation of ...
Biodegradation is now being considered as the best form of cyanide transformation because it is l... more Biodegradation is now being considered as the best form of cyanide transformation because it is less expensive and has fewer hazards than other conventional methods. Soil and drainage water samples from the premises of Univerisiti Putra Malaysia (UPM) Serdang, were collected at different points and analysed for available bacteria. 28 bacteria were isolated from the samples and subjected to in-vitro cyanide remediation assay by using spectrophotometric method. Six bacterial isolates were observed to have cyanide-degrading ability. Screening was conducted in three phases that include primary, secondary and tertiary screening. In the primary phase of the screening, the six bacterial isolates were assayed for cyanide-degrading activity using 25 ppm filter-sterilised KCN in a buffer medium (pH 7.0) containing 5 g/L glucose, and 0.5% yeast extract. It was observed that three isolates have a greater potential of converting cyanide, degrading above 70%. The three isolates were further subje...
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