P. G. Centre of Chemistry (M. U.), College of Commerce, Patna-800 020 <em>Manuscript receiv... more P. G. Centre of Chemistry (M. U.), College of Commerce, Patna-800 020 <em>Manuscript received 19 January 1992, revised 6 July 1992, accepted 25 August 1992</em> Some low-valent organometallic complexes of Pd<sup>0</sup>, Pt<sup>0</sup> and Rh<sup>I</sup> have been prepared and their tentative structure are assigned using various physicochemical data All Pd<sup>O</sup> and Pt<sup>O</sup> compounds are tetrahedral but compounds of Int have square planar configuration. Oxidation state of metals in these compounds are determined iodometrically. The ligand 3-(4 pyridyl) 4-phenyl-1,2,4-triazole-5-thione interacts with its thione tautomeric form with Pd<sup>O</sup> and Pt<sup>O</sup>, but both thione and thiol tautomeric form reacts with Rh! depending on the experimental conditions of the reaction. Metal-ligand vibrations in the far-infrared spectra of complexes have been reasonably assigned.
P G. Centre of Chemistry (M U ), College of Commerce, Patna-800 020 <em>Manuscript received... more P G. Centre of Chemistry (M U ), College of Commerce, Patna-800 020 <em>Manuscript received 22 January 1992, revised 16 December 1992, accepted 19 February 1993</em> Studies on some Mixed-ligand Hydridocarbonyl Phosphine Complexes of lridium(I)
P. G. Centre of Chemistry (M.U.), College of Commerce, Patna-800 020 Manuscript received 22 Janua... more P. G. Centre of Chemistry (M.U.), College of Commerce, Patna-800 020 Manuscript received 22 January 1992, revised 16 December 1992, accepted 19 February 1993 Mixed-ligand hydridocarbonyl and hydridophosphine complexes of Ru<sup>II</sup> and Ir<sup>II</sup> have been isolated from the displacement reaction of [RuH(CO)(PΦ<sub>3</sub>)<sub>3</sub>Cl]on the basis of elemental analysis, electronic, infrared and far-infrared spectral studies. In all cases bonding of INAH occurs through amino nitrogen of hydrazine residue.
Background: Agro-Homeopathy implies the use of Homoeopathy in agriculture. Homoeopathic preparati... more Background: Agro-Homeopathy implies the use of Homoeopathy in agriculture. Homoeopathic preparations are being used efficiently for increasing active principles in medicinal plants, plant detoxification for metals such as aluminum and copper, increasing plant growth rate and productivity, plant metabolism and control of diseases.Objectives1. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on germination of Raphanus sativus in natural environment. 2. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on Plant length of Raphanus sativus in natural environment 3. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on mass of Fresh matter of the shoot and the root system of Raphanus sativus in natural environment Methodology: Seeds were sown in designated area of campus. 30 Plants of Raphanus sativus received Zincum metallicum 6C (20 drops in 1 litre of water) while 30 Plants of Raphanus sativus receiving norma...
This study was aimed at exploring whether latent tuberculosis infection (LTBI) contributes to the... more This study was aimed at exploring whether latent tuberculosis infection (LTBI) contributes to the pathogenesis of immune-mediated inflammatory diseases in a TB endemic setting. We screened 198 rheumatoid arthritis (RA) patients with tuberculin skin test (TST) and studied 61 (median DAS28-ESR = 6.3) who were positive. Whole blood T cell proliferative responses to Mycobacterium tuberculosis (Mtb) membrane (MtM) antigens, including the latency-induced protein alpha crystallin (Acr), were determined by flow cytometry using Ki67 expression as the marker for nuclear proliferation. Serum antibody levels were determined by ELISA. Follow-up investigations (at 3–6, 9–12 and 15–18 months after baseline) were performed in 41 patients who were classified empirically as ‘high’ (HR-T/HR-B) or ‘low’ (LR-T/LR-B) responders based on their dynamic T cell or antibody responses. Significant correlations were seen between baseline T cell responses to MtM and Acr, and between IgG, IgA and IgM antibody res...
Aim: The present study aimed at analysing the probable effects of full mouth oral rehabilitation ... more Aim: The present study aimed at analysing the probable effects of full mouth oral rehabilitation on bite forces at their maximum extent in young paediatric patients with primary as well as mixed dentitions. Methodology: The present study is one of a kind and explores the maximum bite forces in young children. A statistically significant number of children (n = 30) with a mean age of 6.54 years. About 44.75% were boys and 55.25% were girls. The maximum voluntary bite force was assessed for each participant immediately before treatment and 1 month (3–5 weeks) following completion of the needful dental treatment. The difference in bite force magnitude before and after dental treatment was analysed statistically. In addition, the correlations of key variables including, age, height, weight, BMI, gender and caries severity or dental status with maximum bite force were statistically analysed. Results: The mean maximum bite force for the total sample (n = 30) prior to treatment was found to be 167.83 N (SD = 65.20). The mean bite force in the male subgroup was 175.39 N (SD = 64.69) while for the females the mean bite force was equal to 166.29 N (SD = 68.93). Following comprehensive dental treatment, the recorded mean maximum bite force for the children (n = 30) who attended the post-treatment review appointment was 182.60 N (SD = 68.58). Conclusion: The essential factors such as the extent of dental caries, their severity, presence of clinical signs and symptoms has a negative impact on maximum bite force.
Tuberculin skin test (TST) is used most widely for the detection of latent tuberculosis infection... more Tuberculin skin test (TST) is used most widely for the detection of latent tuberculosis infection (LTBI), even though evidences suggest that it could be underreporting the prevalence of LTBI particularly in high disease-burden settings. We have explored whether in vivo (TST) and in vitro (cell-proliferative) T cell responses to PPD can serve as complementary measures. In addition, we also probed whether in vitro T cell response to cell-membrane antigens (Mem) of Mycobacterium tuberculosis (MTB) can serve as a biomarker for LTBI. Study subjects comprised 43 healthcare workers (HCWs), and 9 smear-positive TB patients served as 'disease control'. To measure proliferative T cell responses, 0.1 ml blood (diluted 1:10) was incubated (5 days) with test or control antigen. Cells were stained with fluorescent antibodies to T cell (CD3+/CD4+/CD8+) surface markers and, after fixation and permeabilization, to nuclear proliferation marker Ki67. Data was acquired on a flow cytometer. HCWs...
A synthetic molecule S006-830, belonging to the class of thiophene-containing trisubstituted meth... more A synthetic molecule S006-830, belonging to the class of thiophene-containing trisubstituted methanes, had shown good in vitro and in vivo bactericidal activity against drug-sensitive and drug-resistant (Mtb). The molecule had also shown good druglike pharmacokinetic properties. However, S006-830 is a racemic mixture of two enantiomers, one of which could possess a better pharmacological profile than the other. We purified both the enantiomers on a chiral column and observed that S-enantiomer has a significantly higher inhibitory and cidal activity against Mtb than the R-enantiomer. Action of -S006-830 was "synergistic" for rifampicin and "additive" for isoniazid and ethambutol. The combination of -S006-830 and rifampicin produced 100% kill of Mtb within 8 days. In a chemical proteomics approach using matrix-bound compound to pull down its target protein(s) from Mtb membrane, FabG4 (β-ketoacyl CoA reductase, EC 1.1.1.100) emerged as the most likely target for -S0...
Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-1... more Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to predict the risk of incident of coronary artery disease (CAD). The polymorphism at promoter of TNF-α and IL-10 has been shown to increase transcriptional activity of the gene and play a important role in patho physiology of CAD. Aim of present study is to examine the impact of the TNF-α and IL-10 variant allele on various markers of the CAD and to study its relation with circulating TNF-α and IL-10 levels. The -308 G/A & -238 G/A of TNF-α and -1082 G/A & -819 C/T of IL-10 gene polymorphism has been studied in 301 diagnosed CAD subjects (Age 51.50 ± 9.28; BMI 25.30 ± 3.58) and 305 healthy controls (Age 51.57 ± 9.50; BMI 24.06 ± 7.26). These polymorphism of TNF-α and IL-10 were detected by real time PCR by using Taqman SNP genotyping assay. Furthermore serum TNF-alpha and IL-10 levels were also measured by ELISA. Allelic and genotypic frequencies did...
This study investigated the hypothesis that serum antibodies against Mycobacterium tuberculosis p... more This study investigated the hypothesis that serum antibodies against Mycobacterium tuberculosis present in naturally infected healthy subjects of a tuberculosis (TB) endemic area could create and/or sustain the latent form of infection. All five apparently healthy Indian donors showed high titres of serum antibodies against M. tuberculosis cell membrane antigens, including lipoarabinomannan and alpha crystallin. Uptake and killing of bacilli by the donor macrophages was significantly enhanced following their opsonization with antibody-rich, heat-inactivated autologous sera. However, the capability to opsonize was apparent for antibodies against some and not other antigens. High-content cell imaging of infected macrophages revealed significantly enhanced colocalization of the phagosome maturation marker LAMP-1, though not of calmodulin, with antibody-opsonized compared with unopsonized M. tuberculosis. Key enablers of macrophage microbicidal action-proinflammatory cytokines (IFN-γ an...
P. G. Centre of Chemistry (M. U.), College of Commerce, Patna-800 020 <em>Manuscript receiv... more P. G. Centre of Chemistry (M. U.), College of Commerce, Patna-800 020 <em>Manuscript received 19 January 1992, revised 6 July 1992, accepted 25 August 1992</em> Some low-valent organometallic complexes of Pd<sup>0</sup>, Pt<sup>0</sup> and Rh<sup>I</sup> have been prepared and their tentative structure are assigned using various physicochemical data All Pd<sup>O</sup> and Pt<sup>O</sup> compounds are tetrahedral but compounds of Int have square planar configuration. Oxidation state of metals in these compounds are determined iodometrically. The ligand 3-(4 pyridyl) 4-phenyl-1,2,4-triazole-5-thione interacts with its thione tautomeric form with Pd<sup>O</sup> and Pt<sup>O</sup>, but both thione and thiol tautomeric form reacts with Rh! depending on the experimental conditions of the reaction. Metal-ligand vibrations in the far-infrared spectra of complexes have been reasonably assigned.
P G. Centre of Chemistry (M U ), College of Commerce, Patna-800 020 <em>Manuscript received... more P G. Centre of Chemistry (M U ), College of Commerce, Patna-800 020 <em>Manuscript received 22 January 1992, revised 16 December 1992, accepted 19 February 1993</em> Studies on some Mixed-ligand Hydridocarbonyl Phosphine Complexes of lridium(I)
P. G. Centre of Chemistry (M.U.), College of Commerce, Patna-800 020 Manuscript received 22 Janua... more P. G. Centre of Chemistry (M.U.), College of Commerce, Patna-800 020 Manuscript received 22 January 1992, revised 16 December 1992, accepted 19 February 1993 Mixed-ligand hydridocarbonyl and hydridophosphine complexes of Ru<sup>II</sup> and Ir<sup>II</sup> have been isolated from the displacement reaction of [RuH(CO)(PΦ<sub>3</sub>)<sub>3</sub>Cl]on the basis of elemental analysis, electronic, infrared and far-infrared spectral studies. In all cases bonding of INAH occurs through amino nitrogen of hydrazine residue.
Background: Agro-Homeopathy implies the use of Homoeopathy in agriculture. Homoeopathic preparati... more Background: Agro-Homeopathy implies the use of Homoeopathy in agriculture. Homoeopathic preparations are being used efficiently for increasing active principles in medicinal plants, plant detoxification for metals such as aluminum and copper, increasing plant growth rate and productivity, plant metabolism and control of diseases.Objectives1. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on germination of Raphanus sativus in natural environment. 2. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on Plant length of Raphanus sativus in natural environment 3. To evaluate the effect of Homoeopathic preparations of Zincum metallicum 6C on mass of Fresh matter of the shoot and the root system of Raphanus sativus in natural environment Methodology: Seeds were sown in designated area of campus. 30 Plants of Raphanus sativus received Zincum metallicum 6C (20 drops in 1 litre of water) while 30 Plants of Raphanus sativus receiving norma...
This study was aimed at exploring whether latent tuberculosis infection (LTBI) contributes to the... more This study was aimed at exploring whether latent tuberculosis infection (LTBI) contributes to the pathogenesis of immune-mediated inflammatory diseases in a TB endemic setting. We screened 198 rheumatoid arthritis (RA) patients with tuberculin skin test (TST) and studied 61 (median DAS28-ESR = 6.3) who were positive. Whole blood T cell proliferative responses to Mycobacterium tuberculosis (Mtb) membrane (MtM) antigens, including the latency-induced protein alpha crystallin (Acr), were determined by flow cytometry using Ki67 expression as the marker for nuclear proliferation. Serum antibody levels were determined by ELISA. Follow-up investigations (at 3–6, 9–12 and 15–18 months after baseline) were performed in 41 patients who were classified empirically as ‘high’ (HR-T/HR-B) or ‘low’ (LR-T/LR-B) responders based on their dynamic T cell or antibody responses. Significant correlations were seen between baseline T cell responses to MtM and Acr, and between IgG, IgA and IgM antibody res...
Aim: The present study aimed at analysing the probable effects of full mouth oral rehabilitation ... more Aim: The present study aimed at analysing the probable effects of full mouth oral rehabilitation on bite forces at their maximum extent in young paediatric patients with primary as well as mixed dentitions. Methodology: The present study is one of a kind and explores the maximum bite forces in young children. A statistically significant number of children (n = 30) with a mean age of 6.54 years. About 44.75% were boys and 55.25% were girls. The maximum voluntary bite force was assessed for each participant immediately before treatment and 1 month (3–5 weeks) following completion of the needful dental treatment. The difference in bite force magnitude before and after dental treatment was analysed statistically. In addition, the correlations of key variables including, age, height, weight, BMI, gender and caries severity or dental status with maximum bite force were statistically analysed. Results: The mean maximum bite force for the total sample (n = 30) prior to treatment was found to be 167.83 N (SD = 65.20). The mean bite force in the male subgroup was 175.39 N (SD = 64.69) while for the females the mean bite force was equal to 166.29 N (SD = 68.93). Following comprehensive dental treatment, the recorded mean maximum bite force for the children (n = 30) who attended the post-treatment review appointment was 182.60 N (SD = 68.58). Conclusion: The essential factors such as the extent of dental caries, their severity, presence of clinical signs and symptoms has a negative impact on maximum bite force.
Tuberculin skin test (TST) is used most widely for the detection of latent tuberculosis infection... more Tuberculin skin test (TST) is used most widely for the detection of latent tuberculosis infection (LTBI), even though evidences suggest that it could be underreporting the prevalence of LTBI particularly in high disease-burden settings. We have explored whether in vivo (TST) and in vitro (cell-proliferative) T cell responses to PPD can serve as complementary measures. In addition, we also probed whether in vitro T cell response to cell-membrane antigens (Mem) of Mycobacterium tuberculosis (MTB) can serve as a biomarker for LTBI. Study subjects comprised 43 healthcare workers (HCWs), and 9 smear-positive TB patients served as 'disease control'. To measure proliferative T cell responses, 0.1 ml blood (diluted 1:10) was incubated (5 days) with test or control antigen. Cells were stained with fluorescent antibodies to T cell (CD3+/CD4+/CD8+) surface markers and, after fixation and permeabilization, to nuclear proliferation marker Ki67. Data was acquired on a flow cytometer. HCWs...
A synthetic molecule S006-830, belonging to the class of thiophene-containing trisubstituted meth... more A synthetic molecule S006-830, belonging to the class of thiophene-containing trisubstituted methanes, had shown good in vitro and in vivo bactericidal activity against drug-sensitive and drug-resistant (Mtb). The molecule had also shown good druglike pharmacokinetic properties. However, S006-830 is a racemic mixture of two enantiomers, one of which could possess a better pharmacological profile than the other. We purified both the enantiomers on a chiral column and observed that S-enantiomer has a significantly higher inhibitory and cidal activity against Mtb than the R-enantiomer. Action of -S006-830 was "synergistic" for rifampicin and "additive" for isoniazid and ethambutol. The combination of -S006-830 and rifampicin produced 100% kill of Mtb within 8 days. In a chemical proteomics approach using matrix-bound compound to pull down its target protein(s) from Mtb membrane, FabG4 (β-ketoacyl CoA reductase, EC 1.1.1.100) emerged as the most likely target for -S0...
Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-1... more Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to predict the risk of incident of coronary artery disease (CAD). The polymorphism at promoter of TNF-α and IL-10 has been shown to increase transcriptional activity of the gene and play a important role in patho physiology of CAD. Aim of present study is to examine the impact of the TNF-α and IL-10 variant allele on various markers of the CAD and to study its relation with circulating TNF-α and IL-10 levels. The -308 G/A & -238 G/A of TNF-α and -1082 G/A & -819 C/T of IL-10 gene polymorphism has been studied in 301 diagnosed CAD subjects (Age 51.50 ± 9.28; BMI 25.30 ± 3.58) and 305 healthy controls (Age 51.57 ± 9.50; BMI 24.06 ± 7.26). These polymorphism of TNF-α and IL-10 were detected by real time PCR by using Taqman SNP genotyping assay. Furthermore serum TNF-alpha and IL-10 levels were also measured by ELISA. Allelic and genotypic frequencies did...
This study investigated the hypothesis that serum antibodies against Mycobacterium tuberculosis p... more This study investigated the hypothesis that serum antibodies against Mycobacterium tuberculosis present in naturally infected healthy subjects of a tuberculosis (TB) endemic area could create and/or sustain the latent form of infection. All five apparently healthy Indian donors showed high titres of serum antibodies against M. tuberculosis cell membrane antigens, including lipoarabinomannan and alpha crystallin. Uptake and killing of bacilli by the donor macrophages was significantly enhanced following their opsonization with antibody-rich, heat-inactivated autologous sera. However, the capability to opsonize was apparent for antibodies against some and not other antigens. High-content cell imaging of infected macrophages revealed significantly enhanced colocalization of the phagosome maturation marker LAMP-1, though not of calmodulin, with antibody-opsonized compared with unopsonized M. tuberculosis. Key enablers of macrophage microbicidal action-proinflammatory cytokines (IFN-γ an...
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