MicroRNAs (miRNAs) are important nonprotein-coding genes involved in almost all biological proces... more MicroRNAs (miRNAs) are important nonprotein-coding genes involved in almost all biological processes during biotic and abiotic stresses in plants. To investigate the miRNA-mediated plant response to drought stress, two drought-tolerant (C-306 and NI-5439) and two drought-sensitive (HUW-468 and WL-711) wheat genotypes were exposed to 25 % PEG 6000 for 1, 12 and 24 h. Temporal expression patterns of 12 drought-responsive miRNAs and their corresponding nine targets were monitored by quantitative real-time PCR (qRT-PCR). The results showed differential expression of miRNAs and their targets with varying degree of upregulation and downregulation in drought-sensitive genotypes. Likewise, in drought-tolerant wheat genotypes, maximum accumulation of miR393a and miR397a was observed at 1 h of stress. In addition, nearly perfect negative correlation was observed in four miRNA and target pairs (miR164-NAC, miR168a-AGO, miR398-SOD and miR159a-MYB) across all the temporal period studied which could be a major player during drought response in wheat. We, for the first time, validated the presence of miR529a and miR1029 in wheat. These findings gives a clue for temporal and variety-specific differential regulation of miRNAs and their targets in wheat in response to osmotic shock and could help in defining the potential roles of miRNAs in plant adaptation to osmotic stress in future.
Indian Journal of Experimental Biology, Mar 1, 2010
Hexane, chloroform and ethyl acetate extracts (100 microg/ml) of Alpinia galanga rhizomes exhibit... more Hexane, chloroform and ethyl acetate extracts (100 microg/ml) of Alpinia galanga rhizomes exhibited significant activity in vitro against promastigotes of L. donovani. Twelve compounds namely, methyleugenol (1), p-coumaryl diacetate (2), 1'-acetoxychavicol acetate (3), 1'-acetoxyeugenol acetate (4), trans-p-acetoxycinnamyl alcohol (5), trans-3,4-dimethoxycinnamyl alcohol (6), p-hydroxybenzaldehyde (7), p-hydroxycinnamaldehyde (8), trans-p-coumaryl alcohol (9), galangin (10), trans-p-coumaric acid (11) and galanganol B (12) were isolated from these extracts. Of these, compounds 2, 3, 4 and 5 were found most active in vitro against promastigotes of L. donovani with IC50 values of 39.3, 32.9, 18.9 and 79.9 microM respectively. This is the first report of antileishmanial activity of the extracts and isolated constituents of A. galanga.
Http Dx Doi Org 10 1080 03650340601037127, Nov 27, 2006
Arbuscular mycorrhizae (AM) engage in the most common underground symbiosis that occurs with plan... more Arbuscular mycorrhizae (AM) engage in the most common underground symbiosis that occurs with plants. More than 80% of the higher plants have the ability to form arbuscular mycorrhizal associations. Plants exchange carbohydrates and lipids for nutrients, especially phosphates from AM. Five billion tons of carbon are transferred from plants to fungi via AM each year, worldwide. The fungus in AM symbiosis forms hyphae outside the plant, which provide a connection between the soil and inner part of plant. Phosphorus is transported from the soil solution across the membrane of the hyphae, moves to the arbuscule, unloads from the fungal arbuscule at the arbuscule-cortical interface and is then taken up by the plant cortical cell. AM, in turn, derive carbon from the host plant in the form of hexose/sucrose, which is taken up at the interface and is converted to trehalose and glycogen, typical fungal carbohydrates. This review discusses the transfer of phosphate from soil to plant via AM and the carbon flux and metabolism in AM fungi.
Cloud computing has become a momentous technological trend in recent years, and many experts pres... more Cloud computing has become a momentous technological trend in recent years, and many experts presage that cloud computing could reshape information technology (IT) processes and its current marketplace. These Cloud based Campuses integrated with traditional educational set-ups would help in procuring various solutions helpful not only for students but also for University administration. In this paper, a model for Integrated Campus Management System using Cloud Computing has been proposed for Guru Nanak Dev University's Main Campus and well as its Satellite Campuses.
Inter-satellite optical wireless communication system (IsOWC), one of the important applications ... more Inter-satellite optical wireless communication system (IsOWC), one of the important applications of FSO (Free Space Optics) technology, will be deployed in space in the near future because of providing power efficient and high bandwidth allocation facilities unlike present microwave satellite systems. In this paper, we have deliberated a presentation of different challenging issues in achieving a prolonged inter satellite link for an IsOWC system under different situations and conditions. This work is also emphasized on the suggested techniques to combat with the degrading factors to put into practice of high speed IsOWC system with minimum BER. Index Terms— Inter-satellite optical wireless system (IsOWC), BER, SNR.
Understanding the role of oxidative stress in disease requires real time monitoring of redox stat... more Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.
Both oxidative stress and mitochondrial dysfunction play roles in a myriad of pathological condit... more Both oxidative stress and mitochondrial dysfunction play roles in a myriad of pathological conditions. There is therefore a need for tools that possess the ability to measure the dynamics of oxidative capacity within the mitochondria, particularly those that can measure reversible changes. Here, we report a mitochondrially-targeted fluorescent redox sensor NpFR2, which can reversibly measure changes in the mitochondrial redox environment. The probe has been used to report on variations in oxidative capacity of the haematopoietic cells in bone marrow, thymus and spleen.
Chemical communications (Cambridge, England), Jan 16, 2015
Understanding the role of oxidative stress in disease requires real time monitoring of redox stat... more Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.
MicroRNAs (miRNAs) are important nonprotein-coding genes involved in almost all biological proces... more MicroRNAs (miRNAs) are important nonprotein-coding genes involved in almost all biological processes during biotic and abiotic stresses in plants. To investigate the miRNA-mediated plant response to drought stress, two drought-tolerant (C-306 and NI-5439) and two drought-sensitive (HUW-468 and WL-711) wheat genotypes were exposed to 25 % PEG 6000 for 1, 12 and 24 h. Temporal expression patterns of 12 drought-responsive miRNAs and their corresponding nine targets were monitored by quantitative real-time PCR (qRT-PCR). The results showed differential expression of miRNAs and their targets with varying degree of upregulation and downregulation in drought-sensitive genotypes. Likewise, in drought-tolerant wheat genotypes, maximum accumulation of miR393a and miR397a was observed at 1 h of stress. In addition, nearly perfect negative correlation was observed in four miRNA and target pairs (miR164-NAC, miR168a-AGO, miR398-SOD and miR159a-MYB) across all the temporal period studied which could be a major player during drought response in wheat. We, for the first time, validated the presence of miR529a and miR1029 in wheat. These findings gives a clue for temporal and variety-specific differential regulation of miRNAs and their targets in wheat in response to osmotic shock and could help in defining the potential roles of miRNAs in plant adaptation to osmotic stress in future.
Indian Journal of Experimental Biology, Mar 1, 2010
Hexane, chloroform and ethyl acetate extracts (100 microg/ml) of Alpinia galanga rhizomes exhibit... more Hexane, chloroform and ethyl acetate extracts (100 microg/ml) of Alpinia galanga rhizomes exhibited significant activity in vitro against promastigotes of L. donovani. Twelve compounds namely, methyleugenol (1), p-coumaryl diacetate (2), 1'-acetoxychavicol acetate (3), 1'-acetoxyeugenol acetate (4), trans-p-acetoxycinnamyl alcohol (5), trans-3,4-dimethoxycinnamyl alcohol (6), p-hydroxybenzaldehyde (7), p-hydroxycinnamaldehyde (8), trans-p-coumaryl alcohol (9), galangin (10), trans-p-coumaric acid (11) and galanganol B (12) were isolated from these extracts. Of these, compounds 2, 3, 4 and 5 were found most active in vitro against promastigotes of L. donovani with IC50 values of 39.3, 32.9, 18.9 and 79.9 microM respectively. This is the first report of antileishmanial activity of the extracts and isolated constituents of A. galanga.
Http Dx Doi Org 10 1080 03650340601037127, Nov 27, 2006
Arbuscular mycorrhizae (AM) engage in the most common underground symbiosis that occurs with plan... more Arbuscular mycorrhizae (AM) engage in the most common underground symbiosis that occurs with plants. More than 80% of the higher plants have the ability to form arbuscular mycorrhizal associations. Plants exchange carbohydrates and lipids for nutrients, especially phosphates from AM. Five billion tons of carbon are transferred from plants to fungi via AM each year, worldwide. The fungus in AM symbiosis forms hyphae outside the plant, which provide a connection between the soil and inner part of plant. Phosphorus is transported from the soil solution across the membrane of the hyphae, moves to the arbuscule, unloads from the fungal arbuscule at the arbuscule-cortical interface and is then taken up by the plant cortical cell. AM, in turn, derive carbon from the host plant in the form of hexose/sucrose, which is taken up at the interface and is converted to trehalose and glycogen, typical fungal carbohydrates. This review discusses the transfer of phosphate from soil to plant via AM and the carbon flux and metabolism in AM fungi.
Cloud computing has become a momentous technological trend in recent years, and many experts pres... more Cloud computing has become a momentous technological trend in recent years, and many experts presage that cloud computing could reshape information technology (IT) processes and its current marketplace. These Cloud based Campuses integrated with traditional educational set-ups would help in procuring various solutions helpful not only for students but also for University administration. In this paper, a model for Integrated Campus Management System using Cloud Computing has been proposed for Guru Nanak Dev University's Main Campus and well as its Satellite Campuses.
Inter-satellite optical wireless communication system (IsOWC), one of the important applications ... more Inter-satellite optical wireless communication system (IsOWC), one of the important applications of FSO (Free Space Optics) technology, will be deployed in space in the near future because of providing power efficient and high bandwidth allocation facilities unlike present microwave satellite systems. In this paper, we have deliberated a presentation of different challenging issues in achieving a prolonged inter satellite link for an IsOWC system under different situations and conditions. This work is also emphasized on the suggested techniques to combat with the degrading factors to put into practice of high speed IsOWC system with minimum BER. Index Terms— Inter-satellite optical wireless system (IsOWC), BER, SNR.
Understanding the role of oxidative stress in disease requires real time monitoring of redox stat... more Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.
Both oxidative stress and mitochondrial dysfunction play roles in a myriad of pathological condit... more Both oxidative stress and mitochondrial dysfunction play roles in a myriad of pathological conditions. There is therefore a need for tools that possess the ability to measure the dynamics of oxidative capacity within the mitochondria, particularly those that can measure reversible changes. Here, we report a mitochondrially-targeted fluorescent redox sensor NpFR2, which can reversibly measure changes in the mitochondrial redox environment. The probe has been used to report on variations in oxidative capacity of the haematopoietic cells in bone marrow, thymus and spleen.
Chemical communications (Cambridge, England), Jan 16, 2015
Understanding the role of oxidative stress in disease requires real time monitoring of redox stat... more Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.
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Papers by Amandeep Kaur