A phenomic approach was undertaken to characterize seventy one accessions of sesame (Sesamum indi... more A phenomic approach was undertaken to characterize seventy one accessions of sesame (Sesamum indicum L.) procured from India, Venezuela and USDA following the descriptor of NBPGR and IPGRI. The phenotypic traits varied extensively and the degree of polymorphism was highest in case of seed coat colour while few traits exhibited only binary type character state. Morphological observation revealed Venezuelan genotypes as the most derived ones while the Indian accessions, be it under NBPGR or USDA repository possess some of the wild character state of the traits. The hierarchical axial representation of phenogram indicated that one Indian accession under USDA repository (USIN06) was unique while the Venezuelan genotypes fell in a common cluster in neighbour-joining WPGMA tree. Principal coordinate analysis further resolved the relative distances between genotypes within each cluster/sub cluster. The traits related to trichomes of different plant parts showed highest correlation coeffici...
Indian Journal of Genetics and Plant Breeding (The), 2014
Five exotic sesame accessions were selected by combined phenomic and genomic approaches as prospe... more Five exotic sesame accessions were selected by combined phenomic and genomic approaches as prospective male parents to introgress certain desirable traits as lighter testa colour, synchrony in capsule maturation and non-shattering habit of mature capsules for improvement of one selected Indian accession. One SCAR marker, specific to the female parent (Indian) and certain SSR allelic polymorphism between female and five prospective exotic male parents are reported, which will be helpful for marker assisted breeding of sesame.
The presence of lignans (sesamin, sesamol) adds pharmaceutical value in the sesame oil. Wild sesa... more The presence of lignans (sesamin, sesamol) adds pharmaceutical value in the sesame oil. Wild sesame, Sesamum mulayanum, though less oil-yielding but contains remarkably higher lignan in oil. We performed an interspecific hybridization between cultivated Indian sesame (S. indicum) and S. mulayanum to bring a better oil profile in the cultivated sesame. The selected recombinant lines of F6 generation showed high oil content with a superior lignan profile and manifested distinct phenotypic selection traits. Sesamin synthase is an essential enzyme in the lignan biosynthetic pathway. We studied the gene behind this enzyme during seed development in the parents through semi-quantitative and qRT-PCR analysis. The recombinant lines with high sesamin content showed increased expression of sesamin synthase gene. This gene is a potential candidate for allele-based molecular marker development in future sesame breeding programme.
A 'precocious'/ 'viviparous' seed germination is reported in one Turkish and one ... more A 'precocious'/ 'viviparous' seed germination is reported in one Turkish and one Chinese accession of Sesame (Sesamum indicum L., family Pedaliaceae), an oilseed crop, for the first time. Both the mature and green pods of these accessions showed a peculiar 'within pod' germination of seeds in pot culture. Scanning Electron Microscopy of the placental zone of the immature pod revealed early cotyledon development of different stages, while magnified views of the germinated seedling from the mature pods depicted onset of multi cellular trichome and even the stomata. This unique and aberrant phenomenon of seed germination was correlated with environmental fluctuation associated with low dormancy of seeds.
A phenomic approach was undertaken to characterize seventy one accessions of sesame (Sesamum indi... more A phenomic approach was undertaken to characterize seventy one accessions of sesame (Sesamum indicum L.) procured from India, Venezuela and USDA following the descriptor of NBPGR and IPGRI. The phenotypic traits varied extensively and the degree of polymorphism was highest in case of seed coat colour while few traits exhibited only binary type character state. Morphological observation revealed Venezuelan genotypes as the most derived ones while the Indian accessions, be it under NBPGR or USDA repository possess some of the wild character state of the traits. The hierarchical axial representation of phenogram indicated that one Indian accession under USDA repository (USIN06) was unique while the Venezuelan genotypes fell in a common cluster in neighbour-joining WPGMA tree. Principal coordinate analysis further resolved the relative distances between genotypes within each cluster/sub cluster. The traits related to trichomes of different plant parts showed highest correlation coeffici...
Indian Journal of Genetics and Plant Breeding (The), 2014
Five exotic sesame accessions were selected by combined phenomic and genomic approaches as prospe... more Five exotic sesame accessions were selected by combined phenomic and genomic approaches as prospective male parents to introgress certain desirable traits as lighter testa colour, synchrony in capsule maturation and non-shattering habit of mature capsules for improvement of one selected Indian accession. One SCAR marker, specific to the female parent (Indian) and certain SSR allelic polymorphism between female and five prospective exotic male parents are reported, which will be helpful for marker assisted breeding of sesame.
The presence of lignans (sesamin, sesamol) adds pharmaceutical value in the sesame oil. Wild sesa... more The presence of lignans (sesamin, sesamol) adds pharmaceutical value in the sesame oil. Wild sesame, Sesamum mulayanum, though less oil-yielding but contains remarkably higher lignan in oil. We performed an interspecific hybridization between cultivated Indian sesame (S. indicum) and S. mulayanum to bring a better oil profile in the cultivated sesame. The selected recombinant lines of F6 generation showed high oil content with a superior lignan profile and manifested distinct phenotypic selection traits. Sesamin synthase is an essential enzyme in the lignan biosynthetic pathway. We studied the gene behind this enzyme during seed development in the parents through semi-quantitative and qRT-PCR analysis. The recombinant lines with high sesamin content showed increased expression of sesamin synthase gene. This gene is a potential candidate for allele-based molecular marker development in future sesame breeding programme.
A 'precocious'/ 'viviparous' seed germination is reported in one Turkish and one ... more A 'precocious'/ 'viviparous' seed germination is reported in one Turkish and one Chinese accession of Sesame (Sesamum indicum L., family Pedaliaceae), an oilseed crop, for the first time. Both the mature and green pods of these accessions showed a peculiar 'within pod' germination of seeds in pot culture. Scanning Electron Microscopy of the placental zone of the immature pod revealed early cotyledon development of different stages, while magnified views of the germinated seedling from the mature pods depicted onset of multi cellular trichome and even the stomata. This unique and aberrant phenomenon of seed germination was correlated with environmental fluctuation associated with low dormancy of seeds.
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