Asian Journal of Pharmaceutical Research and Development
Objectives: A crossover-randomized bioequivalence study of two oral formulations of acyclovir 200... more Objectives: A crossover-randomized bioequivalence study of two oral formulations of acyclovir 200 mg tablet was accomplished with 16 adults and physically fit male volunteers from Bangladesh. NOVIRAX™ (Drug International Ltd, Bangladesh) was the test variant whereas ZOVIRAX™ (GlaxoWellcome) was the reference one. Methods: After a night fasting each volunteer was subjected to take a single dosage of tablet with 150 mL of water. After a week for complete washing out, a second dosage was applied. After that, blood samples were collected at a serial interval for a 24 hours period to observe the plasma concentration by HPLC technique. Several pharmacokinetic parameters like Cmax, Tmax, AUC0→24h, t1/2, and Kel were estimated. Results: The mean (± SD) AUC0→24h for acyclovir of test variant NOVIRAXTM for 16 participants was 1057.5 ± 358.9 ng/hr/mL whereas it was 1134.9 ± 467.2 ng/hr/mL for acyclovir of ZOVIRAXTM. The relative bioavailability (NOVIRAXTM/ZOVIRAXTM ratio) was 93.2%. The Cmax, ...
Journal of National Institute of Neurosciences Bangladesh, 2020
Background: Spirulina has several effects in the metabolism of the body. Objective: The purpose o... more Background: Spirulina has several effects in the metabolism of the body. Objective: The purpose of the present study was to see the ability of extracts of spirulina for the removal of arsenic from isolated liver tissues of rat. Methodology: This animal study was carried out on isolated liver tissues of Long Evans Norwegian adult healthy male rats weighing 160 to 200 g. The rats were 3 to 6 months of age obtained from animal house of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh from 2004 to 2005. Measurements and all tasks were performed in a very careful manner. Atomic Absorption Spectrophotometer with Hydride Generator was used to measure the arsenic level. A set of freshly washed test tubes was taken. All test tubes contained 2ml tyrode solution and twenty small pieces of liver tissue maintaining 00C. No arsenic was added in test tube I. 2.5 μg/ml arsenic trioxide was added rest of the test tubes. They all were incubated for 45 minutes at 370C. Then the tissues w...
Bangladesh Medical Research Council bulletin, 2006
To examine the effect of zinc in the removal of accumulated arsenic from different tissues (liver... more To examine the effect of zinc in the removal of accumulated arsenic from different tissues (liver, kidneys, spleen and lungs), rats were initially allowed to drink high concentration (400 microg/kg/day) of arsenic for two months followed by a period of cessation (one month). Administration of zinc (2 mg/kg/day) during the third month in arsenic-treated and non-treated groups were compared. In arsenic-treated rats, the mean (+/- SD) amounts of total arsenic in liver, kidneys, spleen and lungs were 12.3 +/- 0.7, 20.5 +/- 1.0, 31.4 +/- 1.0 and 25.6 +/- 1.1 microg/g of tissues respectively. Administration of zinc to arsenic-treated rats reduced the arsenic concentrations of those tissues to 7.8, 10.7, 23.0 and 14.0 microg/g of tissues. This in vivo study suggests that zinc removes the accumulated arsenic from different tissues significantly (p < 0.001).
A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that... more A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that of innovator product DiflucanTM. The study was conducted on 15 healthy volunteers and single cross-over dose of 150 mg fluconazole was administered orally. Two milliliter of blood was collected at different time intervals for 96 hours. The drug was extracted by liquid-liquid extraction and estimated by high performance liquid chromatography with diode array detector (260 nm). The chromatographic separation was accomplished using C18 analytical column with a mobile phase consisting of water and acetonitril (80:20, v/v). The Cmax of DiflucanTM and FlunacTM was 2.48 ± 0.29 g/mL and 2.23 ± 0.29 g/mL respectively. The Tmax of DiflucanTM and FlunacTM was 2.18 ± 0.98 hours and 2.56 ± 0.81 hours respectively. The area under the curve (0 to 96 hours) of DiflucanTM and FlunacTM was 73.28 ± 13.80 hours/mL and 74.49 ± 16.03 hours/mL. The half-life of both the drugs was 44.59 ± 13.79 hours for Diflu...
Page 1. 53 Total antioxidant content in foodstuffs from arsenic-affected area of Bangladesh Mir M... more Page 1. 53 Total antioxidant content in foodstuffs from arsenic-affected area of Bangladesh Mir Misbahuddin, Anjumanara, Mohammad Al-Amin Division of Arsenic Research Department of Pharmacology Bangabandhu Sheikh Mujib Medical University Shahbag, Dhaka. ...
It is a tragedy that there are still 1.5 billion people on this earth has limited access to drink... more It is a tragedy that there are still 1.5 billion people on this earth has limited access to drinking water. Most of the people are from the developing countries. Another 1.5 billion people are suffering from gastrointestinal diseases due to water-carried pathogens every year. About 3 million people die each year due to gastrointestinal diseases. Arsenic contaminated drinking water is consumed by 50 million people. Five million people are drinking fluoride. Even one million people are consuming lead due to distribution pipes. These pictures are all from the developing countries. In 2002, three-fourth of our total population (72% of the rural population and 82% of the urban population) have the access to safe water. Our focus is on arsenic, fluoride, pesticides, manganese and cyanobacteria.
Bangladesh Medical Research Council Bulletin, 2009
An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A sign... more An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A significant inhibition of gut flora was observed after 2 weeks of administration of arsenic (1 mg/L) ad libitum with a decrease in stool arsenic level and increase in liver arsenic level. However, this inhibitory effect of arsenic on gut flora was not observed in presence of vitamin E (1 mg/day) or selenium (0.4 microg/day). Pretreatment with streptomycin (500 mg twice daily) showed similar results. Rats that received folic acid (200 microg/day) showed inhibition of gut floral count but there were decreased liver arsenic level.
An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A sign... more An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A significant inhibition of gut flora was observed after 2 weeks of administration of arsenic (1 mg/L) ad libitum with a decrease in stool arsenic level and increase in liver arsenic level. However, this inhibitory effect of arsenic on gut flora was not observed in presence of vitamin E (1 mg/day) or selenium (0.4 µg/day). Pretreatment with streptomycin (500 mg twice daily) showed similar results. Rats that received folic acid (200 µg/day) showed inhibition of gut floral count but there were decreased liver arsenic level.
Cesarean section is a common operative procedure in obstetrics. Prophylactic uses of antimicrobia... more Cesarean section is a common operative procedure in obstetrics. Prophylactic uses of antimicrobials in different categories of surgeries are irrational in general, which is also observed in cesarean section. Cost of antimicrobial imposes an enormous burden on the patient or the system and considered as an important cost component of cesarean section. A cross-sectional study was conducted from August 2002 to July 2003 in 8 units of the Departments
This study was carried out to see whether corn extracts could reduce the accumulation of arsenic ... more This study was carried out to see whether corn extracts could reduce the accumulation of arsenic in different tissues of rat. Exposure to arsenic (700µg/rat/day) orally for 15 days led to significant accumulation of arsenic and significant reduction in the concentration of reduced glutathione (GSH) in different tissues. While water, salt, ethanol and alkali extracts of corn were co-administered at a dose of 0.5 mL/rat/day orally by stomach tube during last 8 days, arsenic concentration decreased significantly in all tissues and reduced glutathione (GSH) concentration increased significantly in tissues except heart and skin. Among the extracts, water extract produced maximum reduction of arsenic (69.07 % in liver, 64.98 % in kidney, 63.47 % in lung, 57.55 % in heart and 69.30 % in skin) and elevation of reduced glutathione level in all tissues (17.03 % in liver, 46.73 % in lung, 32.67 % in heart and 55.38 % in skin) except kidney, in which maximum elevation of reduced glutathione was...
The aim of this study was to examine the pattern of fungi in palmar arsenical keratosis. The skin... more The aim of this study was to examine the pattern of fungi in palmar arsenical keratosis. The skin swab was taken from the five different sites (palm, dorsum, axilla, front and back of the chest) of arsenicosis patients (n=37), arsenic exposed volunteers (n=20) and healthy volunteers (n=20) of an arsenic endemic area. Fungi were cultured in the Sabouraud dextrose agar media. The skin of the healthy volunteers contained only four species of fungi: Aspergillus, Dermatophytes, Mucor and Fusarium. The incidence of each fungus was low (0-4 out of 20 cases). The chronic exposure to arsenic did not alter the pattern of fungi. However, the patient of arsenicosis showed increased inci-dence of Aspergillus and Dermatophytes species and decreased incidence of Mucor species. The supplementation of iron and folic acid to the patient for 12 weeks reduced the incidence of Aspergillus (only in axilla), Dermatophytes (only in palm) and increased the incidence of Mucor (all the sites except palm). In ...
Asian Journal of Pharmaceutical Research and Development
Objectives: A crossover-randomized bioequivalence study of two oral formulations of acyclovir 200... more Objectives: A crossover-randomized bioequivalence study of two oral formulations of acyclovir 200 mg tablet was accomplished with 16 adults and physically fit male volunteers from Bangladesh. NOVIRAX™ (Drug International Ltd, Bangladesh) was the test variant whereas ZOVIRAX™ (GlaxoWellcome) was the reference one. Methods: After a night fasting each volunteer was subjected to take a single dosage of tablet with 150 mL of water. After a week for complete washing out, a second dosage was applied. After that, blood samples were collected at a serial interval for a 24 hours period to observe the plasma concentration by HPLC technique. Several pharmacokinetic parameters like Cmax, Tmax, AUC0→24h, t1/2, and Kel were estimated. Results: The mean (± SD) AUC0→24h for acyclovir of test variant NOVIRAXTM for 16 participants was 1057.5 ± 358.9 ng/hr/mL whereas it was 1134.9 ± 467.2 ng/hr/mL for acyclovir of ZOVIRAXTM. The relative bioavailability (NOVIRAXTM/ZOVIRAXTM ratio) was 93.2%. The Cmax, ...
Journal of National Institute of Neurosciences Bangladesh, 2020
Background: Spirulina has several effects in the metabolism of the body. Objective: The purpose o... more Background: Spirulina has several effects in the metabolism of the body. Objective: The purpose of the present study was to see the ability of extracts of spirulina for the removal of arsenic from isolated liver tissues of rat. Methodology: This animal study was carried out on isolated liver tissues of Long Evans Norwegian adult healthy male rats weighing 160 to 200 g. The rats were 3 to 6 months of age obtained from animal house of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh from 2004 to 2005. Measurements and all tasks were performed in a very careful manner. Atomic Absorption Spectrophotometer with Hydride Generator was used to measure the arsenic level. A set of freshly washed test tubes was taken. All test tubes contained 2ml tyrode solution and twenty small pieces of liver tissue maintaining 00C. No arsenic was added in test tube I. 2.5 μg/ml arsenic trioxide was added rest of the test tubes. They all were incubated for 45 minutes at 370C. Then the tissues w...
Bangladesh Medical Research Council bulletin, 2006
To examine the effect of zinc in the removal of accumulated arsenic from different tissues (liver... more To examine the effect of zinc in the removal of accumulated arsenic from different tissues (liver, kidneys, spleen and lungs), rats were initially allowed to drink high concentration (400 microg/kg/day) of arsenic for two months followed by a period of cessation (one month). Administration of zinc (2 mg/kg/day) during the third month in arsenic-treated and non-treated groups were compared. In arsenic-treated rats, the mean (+/- SD) amounts of total arsenic in liver, kidneys, spleen and lungs were 12.3 +/- 0.7, 20.5 +/- 1.0, 31.4 +/- 1.0 and 25.6 +/- 1.1 microg/g of tissues respectively. Administration of zinc to arsenic-treated rats reduced the arsenic concentrations of those tissues to 7.8, 10.7, 23.0 and 14.0 microg/g of tissues. This in vivo study suggests that zinc removes the accumulated arsenic from different tissues significantly (p < 0.001).
A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that... more A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that of innovator product DiflucanTM. The study was conducted on 15 healthy volunteers and single cross-over dose of 150 mg fluconazole was administered orally. Two milliliter of blood was collected at different time intervals for 96 hours. The drug was extracted by liquid-liquid extraction and estimated by high performance liquid chromatography with diode array detector (260 nm). The chromatographic separation was accomplished using C18 analytical column with a mobile phase consisting of water and acetonitril (80:20, v/v). The Cmax of DiflucanTM and FlunacTM was 2.48 ± 0.29 g/mL and 2.23 ± 0.29 g/mL respectively. The Tmax of DiflucanTM and FlunacTM was 2.18 ± 0.98 hours and 2.56 ± 0.81 hours respectively. The area under the curve (0 to 96 hours) of DiflucanTM and FlunacTM was 73.28 ± 13.80 hours/mL and 74.49 ± 16.03 hours/mL. The half-life of both the drugs was 44.59 ± 13.79 hours for Diflu...
Page 1. 53 Total antioxidant content in foodstuffs from arsenic-affected area of Bangladesh Mir M... more Page 1. 53 Total antioxidant content in foodstuffs from arsenic-affected area of Bangladesh Mir Misbahuddin, Anjumanara, Mohammad Al-Amin Division of Arsenic Research Department of Pharmacology Bangabandhu Sheikh Mujib Medical University Shahbag, Dhaka. ...
It is a tragedy that there are still 1.5 billion people on this earth has limited access to drink... more It is a tragedy that there are still 1.5 billion people on this earth has limited access to drinking water. Most of the people are from the developing countries. Another 1.5 billion people are suffering from gastrointestinal diseases due to water-carried pathogens every year. About 3 million people die each year due to gastrointestinal diseases. Arsenic contaminated drinking water is consumed by 50 million people. Five million people are drinking fluoride. Even one million people are consuming lead due to distribution pipes. These pictures are all from the developing countries. In 2002, three-fourth of our total population (72% of the rural population and 82% of the urban population) have the access to safe water. Our focus is on arsenic, fluoride, pesticides, manganese and cyanobacteria.
Bangladesh Medical Research Council Bulletin, 2009
An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A sign... more An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A significant inhibition of gut flora was observed after 2 weeks of administration of arsenic (1 mg/L) ad libitum with a decrease in stool arsenic level and increase in liver arsenic level. However, this inhibitory effect of arsenic on gut flora was not observed in presence of vitamin E (1 mg/day) or selenium (0.4 microg/day). Pretreatment with streptomycin (500 mg twice daily) showed similar results. Rats that received folic acid (200 microg/day) showed inhibition of gut floral count but there were decreased liver arsenic level.
An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A sign... more An in vivo study was carried on rats to see the influence of arsenic on aerobic gut flora. A significant inhibition of gut flora was observed after 2 weeks of administration of arsenic (1 mg/L) ad libitum with a decrease in stool arsenic level and increase in liver arsenic level. However, this inhibitory effect of arsenic on gut flora was not observed in presence of vitamin E (1 mg/day) or selenium (0.4 µg/day). Pretreatment with streptomycin (500 mg twice daily) showed similar results. Rats that received folic acid (200 µg/day) showed inhibition of gut floral count but there were decreased liver arsenic level.
Cesarean section is a common operative procedure in obstetrics. Prophylactic uses of antimicrobia... more Cesarean section is a common operative procedure in obstetrics. Prophylactic uses of antimicrobials in different categories of surgeries are irrational in general, which is also observed in cesarean section. Cost of antimicrobial imposes an enormous burden on the patient or the system and considered as an important cost component of cesarean section. A cross-sectional study was conducted from August 2002 to July 2003 in 8 units of the Departments
This study was carried out to see whether corn extracts could reduce the accumulation of arsenic ... more This study was carried out to see whether corn extracts could reduce the accumulation of arsenic in different tissues of rat. Exposure to arsenic (700µg/rat/day) orally for 15 days led to significant accumulation of arsenic and significant reduction in the concentration of reduced glutathione (GSH) in different tissues. While water, salt, ethanol and alkali extracts of corn were co-administered at a dose of 0.5 mL/rat/day orally by stomach tube during last 8 days, arsenic concentration decreased significantly in all tissues and reduced glutathione (GSH) concentration increased significantly in tissues except heart and skin. Among the extracts, water extract produced maximum reduction of arsenic (69.07 % in liver, 64.98 % in kidney, 63.47 % in lung, 57.55 % in heart and 69.30 % in skin) and elevation of reduced glutathione level in all tissues (17.03 % in liver, 46.73 % in lung, 32.67 % in heart and 55.38 % in skin) except kidney, in which maximum elevation of reduced glutathione was...
The aim of this study was to examine the pattern of fungi in palmar arsenical keratosis. The skin... more The aim of this study was to examine the pattern of fungi in palmar arsenical keratosis. The skin swab was taken from the five different sites (palm, dorsum, axilla, front and back of the chest) of arsenicosis patients (n=37), arsenic exposed volunteers (n=20) and healthy volunteers (n=20) of an arsenic endemic area. Fungi were cultured in the Sabouraud dextrose agar media. The skin of the healthy volunteers contained only four species of fungi: Aspergillus, Dermatophytes, Mucor and Fusarium. The incidence of each fungus was low (0-4 out of 20 cases). The chronic exposure to arsenic did not alter the pattern of fungi. However, the patient of arsenicosis showed increased inci-dence of Aspergillus and Dermatophytes species and decreased incidence of Mucor species. The supplementation of iron and folic acid to the patient for 12 weeks reduced the incidence of Aspergillus (only in axilla), Dermatophytes (only in palm) and increased the incidence of Mucor (all the sites except palm). In ...
Uploads