IOP Conference Series: Earth and Environmental Science
Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteri... more Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteria harbor the ability to form biofilm. One of the natural products that might be potentially applied to treat vibriosis through biofilm deformation is the leaves of Moringa oleifera. The objective of the study was to determine the effect of ethanol extracts of M. oleifera to inhibit V. alginolyticus biofilm formation in vitro. A microtiter plate biofilm assay (OD570nm) method was applied in this study. The treatments were the addition of ethanolic Moringa leaves extracts at different concentrations of 2%, 4%, 8% and 10%. The result of this study showed that Moringa leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The optimal condition to inhibit biofilm formation was at 10% concentration for 60 hours incubation at room temperature signifying the potential application of Moringa leaf extracts to treat biofilmmediated diseases in aquaculture.
Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yiel... more Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yield. The objective of the study was to isolate and identify the etiological agent of vibriosis from the giant freshwater prawn (Macrobrachium rosenbergiii) aquaculture. The study was conducted by isolating the bacteria using selective media of thiosulfate-citrate-bile salts-sucrose (TCBS) agar followed by the morphological and biochemical characterizations, i.e., indole, methyl-red (MR), Voges-Proskauer (VP), citrate, urease, NaCl 0%, gelatinase and lysine decarboxylase tests. The isolated bacteria were further assayed for their standard growth-curve as well as the ability to form a biofilm on Congo-red agar (CRA). The results showed that the bacteria showed a yellow color in colony appearance and had negative Gram staining. On the biochemical assays, the isolates showed positive reactions on the indole, MR, VP, gelatine, and lysine decarboxylase; but had negative reactions on the citrate,...
Koagulasi darah adalah proses pembekuan darah. Koagulasi darah terjadi akibat adanya perlukaan pa... more Koagulasi darah adalah proses pembekuan darah. Koagulasi darah terjadi akibat adanya perlukaan pada pembuluh kapiler untuk mencegah banyaknya darah yang hilang dari pembuluh darah yang rusak. Laju koagulasi pada setiap spesies berbeda-beda. Sejauh ini hanya waktu koagulasi darah pada organisme tingkat mamalia dan aves saja yang sudah diketahui. Tujuan dari percobaan ini adalah untuk mengamati laju penggumpalan darah yang terjadi pada setiap tingkat kelas amphibi, reptil, aves, pisces dan mamalia. Penelitian ini dilakukan dengan metode deskriptif. hasil penelitian yang diperoleh yaitu laju koagulasi darah pada berbagai tingkatan organisme berada dalam kisaran normal yaitu rata-rata 2 menit
IOP Conference Series: Earth and Environmental Science
Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteri... more Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteria harbor the ability to form biofilm. One of the natural products that might be potentially applied to treat vibriosis through biofilm deformation is the leaves of Moringa oleifera. The objective of the study was to determine the effect of ethanol extracts of M. oleifera to inhibit V. alginolyticus biofilm formation in vitro. A microtiter plate biofilm assay (OD570nm) method was applied in this study. The treatments were the addition of ethanolic Moringa leaves extracts at different concentrations of 2%, 4%, 8% and 10%. The result of this study showed that Moringa leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The optimal condition to inhibit biofilm formation was at 10% concentration for 60 hours incubation at room temperature signifying the potential application of Moringa leaf extracts to treat biofilmmediated diseases in aquaculture.
Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yiel... more Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yield. The objective of the study was to isolate and identify the etiological agent of vibriosis from the giant freshwater prawn (Macrobrachium rosenbergiii) aquaculture. The study was conducted by isolating the bacteria using selective media of thiosulfate-citrate-bile salts-sucrose (TCBS) agar followed by the morphological and biochemical characterizations, i.e., indole, methyl-red (MR), Voges-Proskauer (VP), citrate, urease, NaCl 0%, gelatinase and lysine decarboxylase tests. The isolated bacteria were further assayed for their standard growth-curve as well as the ability to form a biofilm on Congo-red agar (CRA). The results showed that the bacteria showed a yellow color in colony appearance and had negative Gram staining. On the biochemical assays, the isolates showed positive reactions on the indole, MR, VP, gelatine, and lysine decarboxylase; but had negative reactions on the citrate,...
Koagulasi darah adalah proses pembekuan darah. Koagulasi darah terjadi akibat adanya perlukaan pa... more Koagulasi darah adalah proses pembekuan darah. Koagulasi darah terjadi akibat adanya perlukaan pada pembuluh kapiler untuk mencegah banyaknya darah yang hilang dari pembuluh darah yang rusak. Laju koagulasi pada setiap spesies berbeda-beda. Sejauh ini hanya waktu koagulasi darah pada organisme tingkat mamalia dan aves saja yang sudah diketahui. Tujuan dari percobaan ini adalah untuk mengamati laju penggumpalan darah yang terjadi pada setiap tingkat kelas amphibi, reptil, aves, pisces dan mamalia. Penelitian ini dilakukan dengan metode deskriptif. hasil penelitian yang diperoleh yaitu laju koagulasi darah pada berbagai tingkatan organisme berada dalam kisaran normal yaitu rata-rata 2 menit
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