Bacteriological examinations of Egyptian currency collected from veterinary markets included micr... more Bacteriological examinations of Egyptian currency collected from veterinary markets included microbial count at 35°C and 25°C, besides that isolation of Escherichia coli (E. coli), Salmonellae, Staphylococcus aureus (S.aureus) and Listeria monocytogenes (L. monocytogenes). The public health significance of isolated organisms and suggestive measures were also discussed. Average bacterial content on the paper currency ranged from10 to10 while the contents on metal coins ranged from 10 to 10 and the 5 8 3 5 more isolated bacteria were S. aureus and E. coli followed by Salmonella spp. while L. monocytogenes was not isolated from the metal coins. The bacterial isolates were sensitive to ofloxacine and ciprofloxacin and more resistant to penicillin, tetracycline, gentamicin, chloramphenicol and amoxicillin.
This study was aimed to determine the humoral immune response that was induced by different Bruce... more This study was aimed to determine the humoral immune response that was induced by different Brucella proteins extract. Humoral immune response was assessed by Rose Bengal test (RBT), indirect ELISA (IELISA) and complement fixation test (CFT) using both smooth and rough Brucella antigens. Six groups of ewes were used to evaluate serological responses following inoculation of soluble Brucella proteins (SBP) and Hot Saline Extract (HSE) that was mixed with or without Montanide-206 in comparison to Rev-1 vaccinated and PBS inoculated groups. With respect to Rev-1 vaccinated group, SBP and HSE induced a satisfactory humoral immune response. Protective activity of these proteins were measured against Egyptian isolate of Brucella melitensis biovar 3 in Balb/C mice where SBP and HSE give significant protection in comparison with Rev-1 vaccine with respect to PBS inoculated control group. Key words:Balb/C Mice HSE Lipopolysaccharide Montanide-206 Periplasmic Rev-1 vaccine SBP S-LPS.
Food-borne bacteria have the most concern in public health and food safety. S. aureus food poison... more Food-borne bacteria have the most concern in public health and food safety. S. aureus food poisoning is one of the most economically important food-borne pathogen worldwide. In the present study a total of 250 food samples (milk samples n=50, white soft cheese samples n=50, yoghurt samples n=50, meat and meat products n=50 and chicken products n=50) were investigated bacteriologically to detect the occurrence of enterotoxigenic S. aureus among the examined food samples. Out of 250 examined food samples, 127 isolates were identified as Staphylococcus species (50.8 %). 32 S. aureus isolates were identified from the examined samples with an incidence of 12.8%. The highest isolation rate was observed in raw milk samples (56%) followed by yoghurt samples (22%), chicken products (6%), white soft cheese samples and pasteurized milk samples (4% each) then meat and meat products samples (2%). Using PCR, out of 32 S. aureus isolated from the examined food samples 10 isolates could produce enterotoxins. Protein profile analysis of 8 enterotoxigenic S. aureus strains were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE). All enterotoxigenic isolates had a band at 26 to 29 kDa. Direct detection of S. aureus enterotoxins were investigated among 6 samples (3 raw milk samples, 2 yoghurt samples and one chicken product sample) using mPCR. It could be concluded that S. aureus producing enterotoxins present in the food samples.
Staphylococcus aureus is an important hospital-and community-associated pathogen that can cause a... more Staphylococcus aureus is an important hospital-and community-associated pathogen that can cause a wide variety of infectious diseases. Methicillin-resistant Staphylococcus aureus (MRSA) generally exhibits multiple resistance patterns to antimicrobial drugs. In the present study 19 S. aureus isolates (9 from bovine and 10 from human) in comparison with the standard Cowan I strain were investigated using antimicrobial sensitivity test and PCR technology. The collected isolates had polymorphisms of the genes encoding staphylococcal coagulase (coa gene) and staphylococci protein A (spa gene) and yielded different PCR products in size ranging from approximately 423 to 658 bp and 396 to 462 bp respectively. 47.4% of S. aureus isolates and the standard Cowan I strain were positive for both sea and seb genes. 57.9% of isolates had a high molecular weight plasmid. S. aureus isolates showed high resistance to methicillin, followed by oxytetracycline, ampicillin and sulphamethoxazole-trimethoprim. Meanwhile 95% of the examined S. aureus isolates were sensitive to vancomycin. All MRSA strains were mecA gene positive by PCR. It is clear that MRSA has been described in bovine and it can easily spread between animals and under certain conditions to humans. This study provides important data on current antimicrobial resistance, including methicillin resistance, for a collection of S. aureus isolated from Egyptian bovine and humans samples.
C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry industry. T... more C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry industry. The first experiment tested the effectiveness of different strains of Lactobacillus as in vitro as probiotic against C. jejuni, E.coli O157 and S. typhimurium Result showed that L.acidophilus isolated from colostrums of mare and goat showed the widest inhibition zone against C. jejuni, E.coli O157 and S. typhimurium strains compared to the use of L.acidophilus isolated from goat and cattle milk. The second experiment evaluate the efficiency of L. acidophilus isolated from mare colostrums showing highest in vitro inhibition activity against tested strains as in vivo probiotic against C. jejuni isolated from broiler carcasses. The result showed great inhibition of C. jejuni, E.coli O157 and S. typhimurium strains by the use of L.acidophilus in comparing to the use of antibiotics.
This study aimed to evaluate soluble brucella proteins as a safe vaccine for control of brucellos... more This study aimed to evaluate soluble brucella proteins as a safe vaccine for control of brucellosis in sheep. Three groups of ewes were used to measure serological responses following inoculation with soluble brucella protein (SBP) mixed with mentonide-206 in comparison to Rev-1 vaccine. 1 group was inoculated with st 8 mg of SBP S/C, 2 group was vaccinated with Rev-1 vaccine at recommended dose S/C where 3 group was nd rd inoculated with saline S/C as control group. Serological responses were measured with Rose Bengal test, modified Rose Bengal Test, CFT and IELISA tests which indicated that SBP give significant antibody responses in a group inoculated with 8 mg SBP mixed with mentonide-206 in a ratio of (1:1) in comparison to Rev-1 vaccinated group. Protective activity of SBP mixed with mentonide-206 was measured against Egyptian isolate of Brucella melitensis biovar 3 in Balb/C mice where SBP gave significant protection in comparison with Rev-1 vaccine with respect of PBS inoculated (unvaccinated) group. The protective activity of SBP was 3.016 while that of Rev-1 was 2.944, accordingly the Rev-1 vaccine is of higher potency than SBP but both are potent.
Bacteriological examinations of Egyptian currency collected from veterinary markets included micr... more Bacteriological examinations of Egyptian currency collected from veterinary markets included microbial count at 35°C and 25°C, besides that isolation of Escherichia coli (E. coli), Salmonellae, Staphylococcus aureus (S.aureus) and Listeria monocytogenes (L. monocytogenes). The public health significance of isolated organisms and suggestive measures were also discussed. Average bacterial content on the paper currency ranged from10 to10 while the contents on metal coins ranged from 10 to 10 and the 5 8 3 5 more isolated bacteria were S. aureus and E. coli followed by Salmonella spp. while L. monocytogenes was not isolated from the metal coins. The bacterial isolates were sensitive to ofloxacine and ciprofloxacin and more resistant to penicillin, tetracycline, gentamicin, chloramphenicol and amoxicillin.
This study was aimed to determine the humoral immune response that was induced by different Bruce... more This study was aimed to determine the humoral immune response that was induced by different Brucella proteins extract. Humoral immune response was assessed by Rose Bengal test (RBT), indirect ELISA (IELISA) and complement fixation test (CFT) using both smooth and rough Brucella antigens. Six groups of ewes were used to evaluate serological responses following inoculation of soluble Brucella proteins (SBP) and Hot Saline Extract (HSE) that was mixed with or without Montanide-206 in comparison to Rev-1 vaccinated and PBS inoculated groups. With respect to Rev-1 vaccinated group, SBP and HSE induced a satisfactory humoral immune response. Protective activity of these proteins were measured against Egyptian isolate of Brucella melitensis biovar 3 in Balb/C mice where SBP and HSE give significant protection in comparison with Rev-1 vaccine with respect to PBS inoculated control group. Key words:Balb/C Mice HSE Lipopolysaccharide Montanide-206 Periplasmic Rev-1 vaccine SBP S-LPS.
Food-borne bacteria have the most concern in public health and food safety. S. aureus food poison... more Food-borne bacteria have the most concern in public health and food safety. S. aureus food poisoning is one of the most economically important food-borne pathogen worldwide. In the present study a total of 250 food samples (milk samples n=50, white soft cheese samples n=50, yoghurt samples n=50, meat and meat products n=50 and chicken products n=50) were investigated bacteriologically to detect the occurrence of enterotoxigenic S. aureus among the examined food samples. Out of 250 examined food samples, 127 isolates were identified as Staphylococcus species (50.8 %). 32 S. aureus isolates were identified from the examined samples with an incidence of 12.8%. The highest isolation rate was observed in raw milk samples (56%) followed by yoghurt samples (22%), chicken products (6%), white soft cheese samples and pasteurized milk samples (4% each) then meat and meat products samples (2%). Using PCR, out of 32 S. aureus isolated from the examined food samples 10 isolates could produce enterotoxins. Protein profile analysis of 8 enterotoxigenic S. aureus strains were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE). All enterotoxigenic isolates had a band at 26 to 29 kDa. Direct detection of S. aureus enterotoxins were investigated among 6 samples (3 raw milk samples, 2 yoghurt samples and one chicken product sample) using mPCR. It could be concluded that S. aureus producing enterotoxins present in the food samples.
Staphylococcus aureus is an important hospital-and community-associated pathogen that can cause a... more Staphylococcus aureus is an important hospital-and community-associated pathogen that can cause a wide variety of infectious diseases. Methicillin-resistant Staphylococcus aureus (MRSA) generally exhibits multiple resistance patterns to antimicrobial drugs. In the present study 19 S. aureus isolates (9 from bovine and 10 from human) in comparison with the standard Cowan I strain were investigated using antimicrobial sensitivity test and PCR technology. The collected isolates had polymorphisms of the genes encoding staphylococcal coagulase (coa gene) and staphylococci protein A (spa gene) and yielded different PCR products in size ranging from approximately 423 to 658 bp and 396 to 462 bp respectively. 47.4% of S. aureus isolates and the standard Cowan I strain were positive for both sea and seb genes. 57.9% of isolates had a high molecular weight plasmid. S. aureus isolates showed high resistance to methicillin, followed by oxytetracycline, ampicillin and sulphamethoxazole-trimethoprim. Meanwhile 95% of the examined S. aureus isolates were sensitive to vancomycin. All MRSA strains were mecA gene positive by PCR. It is clear that MRSA has been described in bovine and it can easily spread between animals and under certain conditions to humans. This study provides important data on current antimicrobial resistance, including methicillin resistance, for a collection of S. aureus isolated from Egyptian bovine and humans samples.
C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry industry. T... more C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry industry. The first experiment tested the effectiveness of different strains of Lactobacillus as in vitro as probiotic against C. jejuni, E.coli O157 and S. typhimurium Result showed that L.acidophilus isolated from colostrums of mare and goat showed the widest inhibition zone against C. jejuni, E.coli O157 and S. typhimurium strains compared to the use of L.acidophilus isolated from goat and cattle milk. The second experiment evaluate the efficiency of L. acidophilus isolated from mare colostrums showing highest in vitro inhibition activity against tested strains as in vivo probiotic against C. jejuni isolated from broiler carcasses. The result showed great inhibition of C. jejuni, E.coli O157 and S. typhimurium strains by the use of L.acidophilus in comparing to the use of antibiotics.
This study aimed to evaluate soluble brucella proteins as a safe vaccine for control of brucellos... more This study aimed to evaluate soluble brucella proteins as a safe vaccine for control of brucellosis in sheep. Three groups of ewes were used to measure serological responses following inoculation with soluble brucella protein (SBP) mixed with mentonide-206 in comparison to Rev-1 vaccine. 1 group was inoculated with st 8 mg of SBP S/C, 2 group was vaccinated with Rev-1 vaccine at recommended dose S/C where 3 group was nd rd inoculated with saline S/C as control group. Serological responses were measured with Rose Bengal test, modified Rose Bengal Test, CFT and IELISA tests which indicated that SBP give significant antibody responses in a group inoculated with 8 mg SBP mixed with mentonide-206 in a ratio of (1:1) in comparison to Rev-1 vaccinated group. Protective activity of SBP mixed with mentonide-206 was measured against Egyptian isolate of Brucella melitensis biovar 3 in Balb/C mice where SBP gave significant protection in comparison with Rev-1 vaccine with respect of PBS inoculated (unvaccinated) group. The protective activity of SBP was 3.016 while that of Rev-1 was 2.944, accordingly the Rev-1 vaccine is of higher potency than SBP but both are potent.
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