Revealing the geological history of microbial life is very challenging. Microbes rarely are prese... more Revealing the geological history of microbial life is very challenging. Microbes rarely are preserved with morphological fidelity, and even when they are, morphology is a poor guide to phylogeny and metabolism. Biological studies of environments considered analogous ...
Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flin... more Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flinders Ranges with a long history of hydrothermal activity. Our aim was to determine the bacterial composition of Paralana using a culture-independent, 16S rRNA-based technique. The presence of a diverse bacterial community was strongly suggested by the large number (~180) of different ribotypes obtained upon analysis of nine hot spring samples. DNA sequencing of Paralana 16S rRNA genes corroborated this observation, identifying representatives of seven confirmed and two candidate divisions of the domain Bacteria. These included Cyanobacteria, Proteobacteria (both β and δ subdivisions), the Cytophaga-Flexibacter-Bacteroides group, Low G+C Gram-positives, Nitrospira, green non-sulfur bacteria, green sulfur bacteria, OP8, and OP12. No known ionizing radiation-resistant Bacteria were identified. Only one Paralana 16S rRNA sequence type (recombinant B5D) was homologous to a sequence previously identified from a radioactive environment.
Proceedings of The International Astronomical Union, 2004
Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flin... more Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flinders Ranges with a long history of hydrothermal activity. Gas bubbling into the pool is composed of radon (from the radioactive decay of radium), nitrogen, carbon dioxide, and trace helium and hydrogen. The microbial composition of mat and biofilm samples from Paralana was determined using culture-independent 16S rRNA techniques. We have previously demonstrated that the hot spring contains a diverse bacterial community. Here we summarise these findings and report on the emph{Archaea} identified in Paralana. Archaeal inhabitants include members of the Crenarchaeota and Euryarchaeota kingdoms.
Revealing the geological history of microbial life is very challenging. Microbes rarely are prese... more Revealing the geological history of microbial life is very challenging. Microbes rarely are preserved with morphological fidelity, and even when they are, morphology is a poor guide to phylogeny and metabolism. Biological studies of environments considered analogous ...
Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flin... more Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flinders Ranges with a long history of hydrothermal activity. Our aim was to determine the bacterial composition of Paralana using a culture-independent, 16S rRNA-based technique. The presence of a diverse bacterial community was strongly suggested by the large number (~180) of different ribotypes obtained upon analysis of nine hot spring samples. DNA sequencing of Paralana 16S rRNA genes corroborated this observation, identifying representatives of seven confirmed and two candidate divisions of the domain Bacteria. These included Cyanobacteria, Proteobacteria (both β and δ subdivisions), the Cytophaga-Flexibacter-Bacteroides group, Low G+C Gram-positives, Nitrospira, green non-sulfur bacteria, green sulfur bacteria, OP8, and OP12. No known ionizing radiation-resistant Bacteria were identified. Only one Paralana 16S rRNA sequence type (recombinant B5D) was homologous to a sequence previously identified from a radioactive environment.
Proceedings of The International Astronomical Union, 2004
Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flin... more Paralana is an active, radon-containing hot spring situated in a region of South Australia's Flinders Ranges with a long history of hydrothermal activity. Gas bubbling into the pool is composed of radon (from the radioactive decay of radium), nitrogen, carbon dioxide, and trace helium and hydrogen. The microbial composition of mat and biofilm samples from Paralana was determined using culture-independent 16S rRNA techniques. We have previously demonstrated that the hot spring contains a diverse bacterial community. Here we summarise these findings and report on the emph{Archaea} identified in Paralana. Archaeal inhabitants include members of the Crenarchaeota and Euryarchaeota kingdoms.
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Papers by Roberto Anitori