Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are qu... more Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are quite restricted and present side effects. The cancer present, mutation wich producing an unrestrained cellular proliferation. Studies about the effects of Low Power Laser Therapy (LPLT) on salivary glands showed an apoptosis increase. So, LLT could be a recommended. therapy Objective: to study histopatologyc an histomorphometric the effects of higher RLBP doses on malignant tumors induced with DMBA on the submandibular glands of rats. Materials and Methods: Sixty (60) male Wistar rats with a corporal weight of ±120 g was divided into two groups of thirty (30) a Control Group (CG) and an Experimental Group (EG). Each animal was anesthetized to surgically expose the submandibular glands wich were injected with 50 uL of DMBA. The rats from the CG were kept alive for 150 days post injection and those from EG after 150 days post injection the glands were exposed and then were irradiated with a...
In recent years there has been increasing interest in the choice of the best material for bone su... more In recent years there has been increasing interest in the choice of the best material for bone substitutes. Experimental models enable estimation of biological potential, efficacy and safety of a biomaterial before its clinical application. The aim of this study was to evaluate the response of a bone substitute, UNC bone matrix powder (MOeP-UNC), for repairing the post-extraction alveolus in Wistar rats. Rats' first lower molars were extracted. The right alveoli were filled with MOeP-UNC hydrated with physiological saline (Experimental Group, EG), and the left alveoli were used as Control Group (CG). Thirty days after extraction, the animals were killed and the samples processed. Histological sections were made in vestibular-lingual direction at the level of the mesial alveolus of the first inferior molar (Guglielmotti et al. J. Oral Maxillofac. Surg. 1985;43(5):359-364). Repair of the alveoli at 30 days after extraction was evaluated histologically. Repair of the alveolus was o...
The present study evaluates the phenotypic and genotypic changes that take place during early onc... more The present study evaluates the phenotypic and genotypic changes that take place during early oncogenesis. The submandibular glands of male rats were injected with a 0.5% solution of 9,10-dimethyl-1,2-benzanthracene (DMBA) in acetone. Gland samples were taken at 0, 7, 30 and 150 days post-injection and submitted to histological, biochemical, immunocytochemical and PCR evaluation. Histopathological analysis was performed on hematoxylin-eosin stained slides. Total protein content was assessed by Lowry's method and the protein profile was analyzed by 12% SDS-PAGE. Bcl-2 was demonstrated by silver-enhanced gold immunolabeling. p53 immunolabeling was performed using the streptavidin-biotin system. All the treated animals developed carcinoma-like lesions at 30 and 150 days. Total protein concentration rose significantly (p < 0.05) above control values at 7, 30 and 150 days. The treated glands exhibited positive immunolabeling for p53 in the nuclei of neoplastic cells at 30 and 150 ...
UNLABELLED Different filling materials have been used in an attempt to repair bone loss situation... more UNLABELLED Different filling materials have been used in an attempt to repair bone loss situations. OBJECTIVE The present study aimed to examine the effect of a bone matrix in post - extraction remodelling of the alveolar bone, and to perform a histomorphometric analysis of the residual alveolar ridges in Wistar rats. MATERIAL AND METHODS Both rat first lower molars were extracted and the right alveoli were filled with particles of a bone matrix with mineral components (MO - UNC) (experimental group, EG). The left alveoli were used as a control group (CG). The animals were sacrificed at 0 hr., 15, 30 and 60 days after extraction, and the samples were processed. Histological sections were made at the level of the mesial alveolus of the first lower molar. Repair of the alveoli was histologically evaluated and a histomorphometric study of total alveolar volume (TAV), height of the buccal plate (Bh), height of the lingual plate (Lh) and percentage of osseointegration (OI) of the particl...
Several bone substitutes have been probed for the replacement and regeneration of damaged tissues... more Several bone substitutes have been probed for the replacement and regeneration of damaged tissues. In dental practice, many situations are considered to require bone substitutes materials to arrive to a successful treatment.
Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are qu... more Salivary gland neoplasms affect the patient´s quality of life. Treatments for these tumors are quite restricted and present side effects. The cancer present, mutation wich producing an unrestrained cellular proliferation. Studies about the effects of Low Power Laser Therapy (LPLT) on salivary glands showed an apoptosis increase. So, LLT could be a recommended. therapy Objective: to study histopatologyc an histomorphometric the effects of higher RLBP doses on malignant tumors induced with DMBA on the submandibular glands of rats. Materials and Methods: Sixty (60) male Wistar rats with a corporal weight of ±120 g was divided into two groups of thirty (30) a Control Group (CG) and an Experimental Group (EG). Each animal was anesthetized to surgically expose the submandibular glands wich were injected with 50 uL of DMBA. The rats from the CG were kept alive for 150 days post injection and those from EG after 150 days post injection the glands were exposed and then were irradiated with a...
In recent years there has been increasing interest in the choice of the best material for bone su... more In recent years there has been increasing interest in the choice of the best material for bone substitutes. Experimental models enable estimation of biological potential, efficacy and safety of a biomaterial before its clinical application. The aim of this study was to evaluate the response of a bone substitute, UNC bone matrix powder (MOeP-UNC), for repairing the post-extraction alveolus in Wistar rats. Rats' first lower molars were extracted. The right alveoli were filled with MOeP-UNC hydrated with physiological saline (Experimental Group, EG), and the left alveoli were used as Control Group (CG). Thirty days after extraction, the animals were killed and the samples processed. Histological sections were made in vestibular-lingual direction at the level of the mesial alveolus of the first inferior molar (Guglielmotti et al. J. Oral Maxillofac. Surg. 1985;43(5):359-364). Repair of the alveoli at 30 days after extraction was evaluated histologically. Repair of the alveolus was o...
The present study evaluates the phenotypic and genotypic changes that take place during early onc... more The present study evaluates the phenotypic and genotypic changes that take place during early oncogenesis. The submandibular glands of male rats were injected with a 0.5% solution of 9,10-dimethyl-1,2-benzanthracene (DMBA) in acetone. Gland samples were taken at 0, 7, 30 and 150 days post-injection and submitted to histological, biochemical, immunocytochemical and PCR evaluation. Histopathological analysis was performed on hematoxylin-eosin stained slides. Total protein content was assessed by Lowry's method and the protein profile was analyzed by 12% SDS-PAGE. Bcl-2 was demonstrated by silver-enhanced gold immunolabeling. p53 immunolabeling was performed using the streptavidin-biotin system. All the treated animals developed carcinoma-like lesions at 30 and 150 days. Total protein concentration rose significantly (p < 0.05) above control values at 7, 30 and 150 days. The treated glands exhibited positive immunolabeling for p53 in the nuclei of neoplastic cells at 30 and 150 ...
UNLABELLED Different filling materials have been used in an attempt to repair bone loss situation... more UNLABELLED Different filling materials have been used in an attempt to repair bone loss situations. OBJECTIVE The present study aimed to examine the effect of a bone matrix in post - extraction remodelling of the alveolar bone, and to perform a histomorphometric analysis of the residual alveolar ridges in Wistar rats. MATERIAL AND METHODS Both rat first lower molars were extracted and the right alveoli were filled with particles of a bone matrix with mineral components (MO - UNC) (experimental group, EG). The left alveoli were used as a control group (CG). The animals were sacrificed at 0 hr., 15, 30 and 60 days after extraction, and the samples were processed. Histological sections were made at the level of the mesial alveolus of the first lower molar. Repair of the alveoli was histologically evaluated and a histomorphometric study of total alveolar volume (TAV), height of the buccal plate (Bh), height of the lingual plate (Lh) and percentage of osseointegration (OI) of the particl...
Several bone substitutes have been probed for the replacement and regeneration of damaged tissues... more Several bone substitutes have been probed for the replacement and regeneration of damaged tissues. In dental practice, many situations are considered to require bone substitutes materials to arrive to a successful treatment.
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Papers by Luis Mario Plavnik