Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intens... more Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intensities on rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and histology digital images were compared for correlation in preand post taxotere treated tumors. For MALDI imaging, iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody (ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.
PET and MW images of mouse prostate tumors were correlated with histology, flow cytometry, DNA fr... more PET and MW images of mouse prostate tumors were correlated with histology, flow cytometry, DNA fragmentation analysis and NMR peaks. Hypotheses were: 1. signal intensities of intracellular sodium (μMRI) and flouro-2-deoxy-glucose utilization (μPET) increased in tumors; 2. image signal intensities were associated with apoptosis as result of DNA fragmentation and accumulation of NMR visible metabolites.PC-3 cell lines were compared with DU-145, LNCaP cell lines in culture for the [Na]i and [Ca]l ion sensing dyes, cell death NMR peaks and apoptosis staining for chemotherapeutic action of different drugs. After PC3 tumor imaging, taxotere (40 mg/kg; n=5) and VP-16 etoposide (1.2 mg/kg; n=7) was administered i.v. and imaging was done after 12 hours and 24 hours. Tumors were taken out for immunohistological staining by pentachrome, feulgen and ss-DNA antibody. μPET and μMRI images showed increased 18-FDG uptake and sodium signal intensities in tumor. In tumors, taxotere induced an increas...
Abstract: Integration of imaging data with immunohistology is a new art. Increased PET and MRI im... more Abstract: Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intensities on rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and histology digital images were compared for correlation in pre- and post taxotere treated tumors. For MALDI imaging, iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody (ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.
Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intens... more Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intensities on rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and histology digital images were compared for correlation in preand post taxotere treated tumors. For MALDI imaging, iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody (ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.
PET and MW images of mouse prostate tumors were correlated with histology, flow cytometry, DNA fr... more PET and MW images of mouse prostate tumors were correlated with histology, flow cytometry, DNA fragmentation analysis and NMR peaks. Hypotheses were: 1. signal intensities of intracellular sodium (μMRI) and flouro-2-deoxy-glucose utilization (μPET) increased in tumors; 2. image signal intensities were associated with apoptosis as result of DNA fragmentation and accumulation of NMR visible metabolites.PC-3 cell lines were compared with DU-145, LNCaP cell lines in culture for the [Na]i and [Ca]l ion sensing dyes, cell death NMR peaks and apoptosis staining for chemotherapeutic action of different drugs. After PC3 tumor imaging, taxotere (40 mg/kg; n=5) and VP-16 etoposide (1.2 mg/kg; n=7) was administered i.v. and imaging was done after 12 hours and 24 hours. Tumors were taken out for immunohistological staining by pentachrome, feulgen and ss-DNA antibody. μPET and μMRI images showed increased 18-FDG uptake and sodium signal intensities in tumor. In tumors, taxotere induced an increas...
Abstract: Integration of imaging data with immunohistology is a new art. Increased PET and MRI im... more Abstract: Integration of imaging data with immunohistology is a new art. Increased PET and MRI image intensities on rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and histology digital images were compared for correlation in pre- and post taxotere treated tumors. For MALDI imaging, iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody (ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.
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Papers by Rakesh Sharma
rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging
tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium
MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were
positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and
histology digital images were compared for correlation in pre- and post taxotere treated tumors. For MALDI imaging,
iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence
and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy
by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology
and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated
by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal
intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in
IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than
that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and
apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation
index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody
(ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their
distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match
with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging
integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.
rat breast tumor MRI-PET images were reviewed for possible correlation with tumor histology and MALDI imaging
tumor characteristics in the light of recent inventions and patents. Increased signal intensities of intracellular (IC) sodium
MRI and flouro-2-deoxy-glucose utilization by PET from apoptosis protein rich MALDI visible regions of tumors were
positively correlated to chemosensitivity of Taxotere. MCF-7 cancer cell line induced rat tumor MRI-PET images and
histology digital images were compared for correlation in pre- and post taxotere treated tumors. For MALDI imaging,
iterated protein ion mass spectrometry peak analysis was done using data from laser raster over tumor slices in sequence
and 3D tumor volume was simulated for specific peak(s) distribution. A criterion was developed to evaluate malignancy
by histology and MRI-PET imaging. For correlation, regression analysis was done using MRI-PET imaging, histology
and MALDI imaging data from MCF-7 tumor after 24 hours post-taxotere treatment. Apoptosis indices were calculated
by histostaining using pentachrome, feulgen and ss-DNA antibody assay. Review showed sodium MRI and PET signal
intensity distribution comparable and measurable in tumor tissue regions. In tumors, taxotere induced an increase in
IC-Na MRI signal with decreased tumor size and micro-PET showed FDG uptake increase with decreased tumor size than
that of control tumors after 24 hours. Histology features indicated tumor risk (high 'IC/EC ratio', high mitotic index and
apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy and proliferation
index) after Taxotere treatment. These features in co-registered IC-Na, PET hypermetabolic and monoclonal antibody
(ss-DNA) sensitive regions showed 6% difference. MALDI imaging showed tumor specific protein ion species and their
distribution showed empirical correlation (limited visual match) with MRI-PET signal intensities but comparable match
with histology features. Recent patents strongly suggest the possibility of sodium MRI and PET multimodal imaging
integrated with MALDI-imaging as an non-invasive chemosensitivity assay to monitor the anticancer effect.