Palabras clave: Melittis melissophyllum L. subsp. melissophyllum, Labiatae, aceite esencial, cuma... more Palabras clave: Melittis melissophyllum L. subsp. melissophyllum, Labiatae, aceite esencial, cumarina, desorcion termica, Espana. Abstract: Velasco-Negueruela, A.; Sanz, J.; Perez-Alonso, M. J. & Pala-Paul, J. 2004. The volatile components of the aerial parts of Melittis melissophyllum L. subsp. melissophyllum gathered in Spain. Bot. Complut. 28: 133-137. The volatiles obtained by hydrodistillation and thermal desorption (TD) from the aerial parts of Melittis melissophylum subsp. melissophyllum were studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The oil from the fresh plant was shown to contain 8,11,14-eicosatrienoic acid (31.4%), hexadecanoic acid (10.9%), bicyclogermacrene (8.1%), ger- macrene D (7.1%) and b-caryophyllene (7.0%) as main constituents and minor amounts of 1-octen-3-ol (3.9%) and phytol (2.8%), whereas the oil from the air dried plant had 8,11,14-eicosatrienoic acid (29.1%), hexadecanoic acid (21.4%), phytol (16.0%) and spa- thul...
Se denomina “quimiotipo” a un grupo de individuos de una especie que se distingue de forma signif... more Se denomina “quimiotipo” a un grupo de individuos de una especie que se distingue de forma significativa del resto por su composicion quimica. Mientras que la existencia de polimorfismo quimico es bien conocida para diversas especies, el significado estadistico de la diferencia entre sus composiciones no ha recibido suficiente atencion. Se propone, para la asignacion de un nivel de significado a la existencia de un quimiotipo caracterizado por un compuesto, un metodo basado en la formacion de agrupaciones mediante el procedimiento de k-medias, el calculo de la distancia de Mahalanobis Dmah como estimacion de la separacion entre grupos, y la comparacion de este valor con valores Dmah calculados a partir de datos simulados con una distribucion normal. El metodo se aplica a los datos de concentracion obtenidos por GC-MS de compuestos volatiles de muestras de Thymus zygis subsp. zygis, Thymus zygis subsp. sylvestris y Lavandula luisieri.
The chemical composition and biological activity of cultivated and wild medicinal and aromatic pl... more The chemical composition and biological activity of cultivated and wild medicinal and aromatic plants from Morocco ( Artemisia herba-alba, Lippia citriodora, Mentha pulegium, M. spicata, Myrtus communis, Rosmarinus officinalis, and Thymus satureioides) are described. The essential oils (EOs) of these species have been analyzed by GC-MS. The antifeedant, nematicidal and phytotoxic activities of the EOs were tested on insect pests ( Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi), root-knot nematodes ( Meloydogine javanica) and plants ( Lactuca sativa, Lolium perenne and Lycopersicum esculentum). EOs from A. herba-alba, M. pulegium and R. officinalis were strong antifeedants against S. littoralis, M. persicae and R. padi. EOs from L. citriodora, M. spicata and T. satureioides showed high nematicidal activity. These biological effects are explained by the activity of the major EO components and/or synergistic effects.
A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by m... more A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrich...
An improved method has been developed to identify and quantify honey disaccharides and trisacchar... more An improved method has been developed to identify and quantify honey disaccharides and trisaccharides by gas chromatography and mass spectrometry. The procedure, based on mass spectral and retention data (“retention time windows”) determined on two capillary columns with different stationary phases allowed the identification and quantitation in honey of 16 disaccharides and 9 trisaccharides, some of which were not previously
Una revisión sobre la enfermedad de Chagas pone de manifiesto los efectos repelentes e insecticid... more Una revisión sobre la enfermedad de Chagas pone de manifiesto los efectos repelentes e insecticidas que ejercen algunos aceites esenciales sobre los insectos que transmiten el parásito causante de esta enfermedad
Abstract Fifty-six components have been identified in the essential oils from 12 samples of Sider... more Abstract Fifty-six components have been identified in the essential oils from 12 samples of Sideritis hirsuta . The correlations among their concentrations in the oils are also discussed.
The pH-dependent forms adopted by the xanthene dye succinylfluorescein in 1:1 v/v aqueous methano... more The pH-dependent forms adopted by the xanthene dye succinylfluorescein in 1:1 v/v aqueous methanol buffers have been deduced from factor analysis of absorbance data measured at six wavelengths in the visible region, and sixteen pH-values. It is concluded that a protonated species and a doubly-charged anion are the only absorbing species at the two ends of the pH-range studied, 1.40-9.77, and the quinonoid neutral and singly charged anion forms, both with similar absorption properties, are the major components in the pH-range 5-6. A colourless minor compound may also be present at around pH 4. Apparent pK values corresponding to the three ionizations involved are 2.90, 4.60 and 6.80, as determined by potentiometry.
Volatile compounds have been investigated in unifloral honeys of the most popular types in Spain.... more Volatile compounds have been investigated in unifloral honeys of the most popular types in Spain. A total of 21 eucalyptus samples, 35 rosemary samples, 33 heather samples, and 15 citrus samples were collected in the course of 3 years in different Spanish regions. Samples were analyzed by SPME followed by GC-MS. About 83 compounds were identified; the concentrations of 33 of them were selected to be processed by multivariate analysis. Discriminant analysis allowed correct assignment of most samples: 94% citrus, 92% eucalyptus, 84% heather, and 84% rosemary. As pure reference honeys are not available, a two step data analysis is proposed, selecting the samples most clearly classified in discriminant analysis as "reference samples" to be used in multiple regression to estimate the most representative compounds for each honey type and considering the rest of the samples as of mixed origin.
A solid-phase microextraction (SPME)-based method for the GC-MS analysis of volatiles in dehydrat... more A solid-phase microextraction (SPME)-based method for the GC-MS analysis of volatiles in dehydrated carrot root samples has been developed and the effect of the most important factors (fibre coating, extraction temperature, equilibrium time and extraction time) on the fractionation of different volatiles has been studied. GC-MS chromatograms showed terpenoids relevant to carrot aroma such as alpha-pinene, sabinene, beta-myrcene, limonene, gamma-terpinene, terpinolene, trans-caryophyllene and beta-bisabolene, and several furan derivatives whose origin is discussed in this paper. As an example of application, this methodology has been used for the characterization of volatile composition of industrially dehydrated carrots. SPME followed by GC-MS is shown as an affordable, fast and solvent-free technique which can be performed with low sample amounts and be easily implemented at an industry for quality control purposes.
... The Essential Oil of Salvia gilliesii Benth. Arturo Velasco-Negueruela" and Maria JosB P... more ... The Essential Oil of Salvia gilliesii Benth. Arturo Velasco-Negueruela" and Maria JosB PBrez-Alonso @to. ... 1. G. A. Fester, E. A. Martinuzzi, J. A. Retamar and A. J. Ricciardi, Aceites Esenciales de la Repziblica Argentina. Academia Naciond de Ciencias, C6rdoba (RA) (1961). ...
... Auteur(s) / Author(s). PALA-PAUL Jesus ; PEREZ-ALONSO José ; VELASCO-NEGUERUELA Arturo ; VILL... more ... Auteur(s) / Author(s). PALA-PAUL Jesus ; PEREZ-ALONSO José ; VELASCO-NEGUERUELA Arturo ; VILLA Ana Maria ; GRANDA Elena ; SANZ Jesus ; Résumé / Abstract. ... Langue / Language. Anglais Editeur / Publisher. Allured, Carol Stream, IL, ETATS-UNIS (1989) (Revue) ...
Palabras clave: Melittis melissophyllum L. subsp. melissophyllum, Labiatae, aceite esencial, cuma... more Palabras clave: Melittis melissophyllum L. subsp. melissophyllum, Labiatae, aceite esencial, cumarina, desorcion termica, Espana. Abstract: Velasco-Negueruela, A.; Sanz, J.; Perez-Alonso, M. J. & Pala-Paul, J. 2004. The volatile components of the aerial parts of Melittis melissophyllum L. subsp. melissophyllum gathered in Spain. Bot. Complut. 28: 133-137. The volatiles obtained by hydrodistillation and thermal desorption (TD) from the aerial parts of Melittis melissophylum subsp. melissophyllum were studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The oil from the fresh plant was shown to contain 8,11,14-eicosatrienoic acid (31.4%), hexadecanoic acid (10.9%), bicyclogermacrene (8.1%), ger- macrene D (7.1%) and b-caryophyllene (7.0%) as main constituents and minor amounts of 1-octen-3-ol (3.9%) and phytol (2.8%), whereas the oil from the air dried plant had 8,11,14-eicosatrienoic acid (29.1%), hexadecanoic acid (21.4%), phytol (16.0%) and spa- thul...
Se denomina “quimiotipo” a un grupo de individuos de una especie que se distingue de forma signif... more Se denomina “quimiotipo” a un grupo de individuos de una especie que se distingue de forma significativa del resto por su composicion quimica. Mientras que la existencia de polimorfismo quimico es bien conocida para diversas especies, el significado estadistico de la diferencia entre sus composiciones no ha recibido suficiente atencion. Se propone, para la asignacion de un nivel de significado a la existencia de un quimiotipo caracterizado por un compuesto, un metodo basado en la formacion de agrupaciones mediante el procedimiento de k-medias, el calculo de la distancia de Mahalanobis Dmah como estimacion de la separacion entre grupos, y la comparacion de este valor con valores Dmah calculados a partir de datos simulados con una distribucion normal. El metodo se aplica a los datos de concentracion obtenidos por GC-MS de compuestos volatiles de muestras de Thymus zygis subsp. zygis, Thymus zygis subsp. sylvestris y Lavandula luisieri.
The chemical composition and biological activity of cultivated and wild medicinal and aromatic pl... more The chemical composition and biological activity of cultivated and wild medicinal and aromatic plants from Morocco ( Artemisia herba-alba, Lippia citriodora, Mentha pulegium, M. spicata, Myrtus communis, Rosmarinus officinalis, and Thymus satureioides) are described. The essential oils (EOs) of these species have been analyzed by GC-MS. The antifeedant, nematicidal and phytotoxic activities of the EOs were tested on insect pests ( Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi), root-knot nematodes ( Meloydogine javanica) and plants ( Lactuca sativa, Lolium perenne and Lycopersicum esculentum). EOs from A. herba-alba, M. pulegium and R. officinalis were strong antifeedants against S. littoralis, M. persicae and R. padi. EOs from L. citriodora, M. spicata and T. satureioides showed high nematicidal activity. These biological effects are explained by the activity of the major EO components and/or synergistic effects.
A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by m... more A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrich...
An improved method has been developed to identify and quantify honey disaccharides and trisacchar... more An improved method has been developed to identify and quantify honey disaccharides and trisaccharides by gas chromatography and mass spectrometry. The procedure, based on mass spectral and retention data (“retention time windows”) determined on two capillary columns with different stationary phases allowed the identification and quantitation in honey of 16 disaccharides and 9 trisaccharides, some of which were not previously
Una revisión sobre la enfermedad de Chagas pone de manifiesto los efectos repelentes e insecticid... more Una revisión sobre la enfermedad de Chagas pone de manifiesto los efectos repelentes e insecticidas que ejercen algunos aceites esenciales sobre los insectos que transmiten el parásito causante de esta enfermedad
Abstract Fifty-six components have been identified in the essential oils from 12 samples of Sider... more Abstract Fifty-six components have been identified in the essential oils from 12 samples of Sideritis hirsuta . The correlations among their concentrations in the oils are also discussed.
The pH-dependent forms adopted by the xanthene dye succinylfluorescein in 1:1 v/v aqueous methano... more The pH-dependent forms adopted by the xanthene dye succinylfluorescein in 1:1 v/v aqueous methanol buffers have been deduced from factor analysis of absorbance data measured at six wavelengths in the visible region, and sixteen pH-values. It is concluded that a protonated species and a doubly-charged anion are the only absorbing species at the two ends of the pH-range studied, 1.40-9.77, and the quinonoid neutral and singly charged anion forms, both with similar absorption properties, are the major components in the pH-range 5-6. A colourless minor compound may also be present at around pH 4. Apparent pK values corresponding to the three ionizations involved are 2.90, 4.60 and 6.80, as determined by potentiometry.
Volatile compounds have been investigated in unifloral honeys of the most popular types in Spain.... more Volatile compounds have been investigated in unifloral honeys of the most popular types in Spain. A total of 21 eucalyptus samples, 35 rosemary samples, 33 heather samples, and 15 citrus samples were collected in the course of 3 years in different Spanish regions. Samples were analyzed by SPME followed by GC-MS. About 83 compounds were identified; the concentrations of 33 of them were selected to be processed by multivariate analysis. Discriminant analysis allowed correct assignment of most samples: 94% citrus, 92% eucalyptus, 84% heather, and 84% rosemary. As pure reference honeys are not available, a two step data analysis is proposed, selecting the samples most clearly classified in discriminant analysis as "reference samples" to be used in multiple regression to estimate the most representative compounds for each honey type and considering the rest of the samples as of mixed origin.
A solid-phase microextraction (SPME)-based method for the GC-MS analysis of volatiles in dehydrat... more A solid-phase microextraction (SPME)-based method for the GC-MS analysis of volatiles in dehydrated carrot root samples has been developed and the effect of the most important factors (fibre coating, extraction temperature, equilibrium time and extraction time) on the fractionation of different volatiles has been studied. GC-MS chromatograms showed terpenoids relevant to carrot aroma such as alpha-pinene, sabinene, beta-myrcene, limonene, gamma-terpinene, terpinolene, trans-caryophyllene and beta-bisabolene, and several furan derivatives whose origin is discussed in this paper. As an example of application, this methodology has been used for the characterization of volatile composition of industrially dehydrated carrots. SPME followed by GC-MS is shown as an affordable, fast and solvent-free technique which can be performed with low sample amounts and be easily implemented at an industry for quality control purposes.
... The Essential Oil of Salvia gilliesii Benth. Arturo Velasco-Negueruela" and Maria JosB P... more ... The Essential Oil of Salvia gilliesii Benth. Arturo Velasco-Negueruela" and Maria JosB PBrez-Alonso @to. ... 1. G. A. Fester, E. A. Martinuzzi, J. A. Retamar and A. J. Ricciardi, Aceites Esenciales de la Repziblica Argentina. Academia Naciond de Ciencias, C6rdoba (RA) (1961). ...
... Auteur(s) / Author(s). PALA-PAUL Jesus ; PEREZ-ALONSO José ; VELASCO-NEGUERUELA Arturo ; VILL... more ... Auteur(s) / Author(s). PALA-PAUL Jesus ; PEREZ-ALONSO José ; VELASCO-NEGUERUELA Arturo ; VILLA Ana Maria ; GRANDA Elena ; SANZ Jesus ; Résumé / Abstract. ... Langue / Language. Anglais Editeur / Publisher. Allured, Carol Stream, IL, ETATS-UNIS (1989) (Revue) ...
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