Self-splicing group II introns are large ribozymes and mobile retroelements initially identified ... more Self-splicing group II introns are large ribozymes and mobile retroelements initially identified in the mitochondrial and chloroplast genomes of lower eukaryotes and plants and subsequently found in bacteria and archaea. Group II introns display structural, functional and mechanistic similarities to eukaryotic pre-mRNA nuclear introns, which may have evolved from mobile group II introns. As in spliceosomal introns, the ribozyme of group II introns excises the intron as a branched, lariat structure, through two sequential transesterification reactions. The movement of group II introns is mediated by a ribonucleoprotein (RNP) complex consisting of the IEP encoded by the ORF and the spliced intron lariat RNA, which remains associated with the IEP. These RNP complexes recognize intron targets through both the IEP and the intron lariat RNA. New possibilities for the use of these introns as biotechnological tools are emerging, due to the small number and flexibility of interactions between IEPs and target sites, through the modification of the intron RNA motifs that recognize DNA target sites by base pairing.
Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcri... more Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcriptase. Here, we provide methods for their identification in bacterial genomes and further analysis of their splicing and mobility capacities.
Current plant science and biotechnology in agriculture, 1998
The low performance of alfalfa and other leguminous plants in soils with low pH is due to several... more The low performance of alfalfa and other leguminous plants in soils with low pH is due to several factors that affect the plant host, the rhizobia native population and the symbiotic interaction itself. The selection of more tolerant rhizobia to soil acidity has appeared as a plausible approach to solve alfalfa productivity at low pH. A survay for indigenous rhizobia population nodulating this forage legume in Argentina and Uruguay has been performed.
ABSTRACT SynonymsApplications of metagenomics to rhizosphere studiesDefinitionThe rhizosphere is ... more ABSTRACT SynonymsApplications of metagenomics to rhizosphere studiesDefinitionThe rhizosphere is defined as the portion of soil where microorganism-mediated processes are under the influence of the plant root system. Originally described as an effect of the roots, nowadays the rhizosphere is considered as an ecosystem, and, as such, it has one of the greater impacts on the functioning of the biosphere due to its extension and to the energy consumption of living organisms. The “metagenomic approach” opens a novel era for unraveling one of the most complex, ubiquitous, and, however, hidden ecosystem.IntroductionThe rhizosphere constitutes an ecological niche where complex microbial communities are supported by nutrients released from the root system, including exudates, mucilage, and sloughed-off root cells. Classically, the rhizosphere has been defined as the volume of soil over which plant roots exert an influence. It differs from bulk soil due to the biological and physicochemical processes th ...
Self-splicing group II introns are large ribozymes and mobile retroelements initially identified ... more Self-splicing group II introns are large ribozymes and mobile retroelements initially identified in the mitochondrial and chloroplast genomes of lower eukaryotes and plants and subsequently found in bacteria and archaea. Group II introns display structural, functional and mechanistic similarities to eukaryotic pre-mRNA nuclear introns, which may have evolved from mobile group II introns. As in spliceosomal introns, the ribozyme of group II introns excises the intron as a branched, lariat structure, through two sequential transesterification reactions. The movement of group II introns is mediated by a ribonucleoprotein (RNP) complex consisting of the IEP encoded by the ORF and the spliced intron lariat RNA, which remains associated with the IEP. These RNP complexes recognize intron targets through both the IEP and the intron lariat RNA. New possibilities for the use of these introns as biotechnological tools are emerging, due to the small number and flexibility of interactions between IEPs and target sites, through the modification of the intron RNA motifs that recognize DNA target sites by base pairing.
Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcri... more Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcriptase. Here, we provide methods for their identification in bacterial genomes and further analysis of their splicing and mobility capacities.
Current plant science and biotechnology in agriculture, 1998
The low performance of alfalfa and other leguminous plants in soils with low pH is due to several... more The low performance of alfalfa and other leguminous plants in soils with low pH is due to several factors that affect the plant host, the rhizobia native population and the symbiotic interaction itself. The selection of more tolerant rhizobia to soil acidity has appeared as a plausible approach to solve alfalfa productivity at low pH. A survay for indigenous rhizobia population nodulating this forage legume in Argentina and Uruguay has been performed.
ABSTRACT SynonymsApplications of metagenomics to rhizosphere studiesDefinitionThe rhizosphere is ... more ABSTRACT SynonymsApplications of metagenomics to rhizosphere studiesDefinitionThe rhizosphere is defined as the portion of soil where microorganism-mediated processes are under the influence of the plant root system. Originally described as an effect of the roots, nowadays the rhizosphere is considered as an ecosystem, and, as such, it has one of the greater impacts on the functioning of the biosphere due to its extension and to the energy consumption of living organisms. The “metagenomic approach” opens a novel era for unraveling one of the most complex, ubiquitous, and, however, hidden ecosystem.IntroductionThe rhizosphere constitutes an ecological niche where complex microbial communities are supported by nutrients released from the root system, including exudates, mucilage, and sloughed-off root cells. Classically, the rhizosphere has been defined as the volume of soil over which plant roots exert an influence. It differs from bulk soil due to the biological and physicochemical processes th ...
Uploads