I am research oriented professional working in the field of Plant Biotechnology. My main work area is on assessment of molecular systematics, biodiversity conservation, genetic diversity, population genetics and DNA fingerprinting of plants using PCR based methods. I am also interested and working on Plant molecular breeding, Plant phylogeny and evolutionary biology.
Genetic variability and population structure of Bergenia ciliata (Haw.) Sternb., commonly known a... more Genetic variability and population structure of Bergenia ciliata (Haw.) Sternb., commonly known as “ Pashanbheda ” (Stone-breaker), collected from the Western Himalayan region of India were estimated using two DNA fingerprinting methods viz ., directed amplification of minisatellite DNA (DAMD) and inter simple sequence repeats (ISSR). The cumulative data analysis of DAMD and ISSR markers for 74 accessions from eight populations showed 86.1% polymorphism. Analysis of molecular variance (AMOVA) showed highest percentage of variation within individuals of populations (73.6%) and 21.7% among populations. STRUCTURE and PCoA analyses on the hierarchical partitioning of genetic diversity showed strong admixture of individuals among the eight assumed geographical populations of B. ciliata . The data suggests that high genetic flow is one of the major factors responsible for low genetic differentiation. Preservation of genetic diversity of B. ciliata is important, both to promote adaptabilit...
Indian Journal of Natural Products and Resources, 2017
Other than the aesthetic values, orchids, the largest and most diverse group of flowering plants ... more Other than the aesthetic values, orchids, the largest and most diverse group of flowering plants has a broad range of ethnobotanical applications. However, little work has been done on ethnobotanical studies of orchids used by the Khamti community of the North-East India. The present report is an account of the ethnobotanical uses of 4 medicinal orchids, viz., Cymbidiun aloifolium (Linn.) Sw., Cymbidium bicolor Lindl., Eria pannea Lindl., and Dendrobium fimbriatum Hook. among the Khamti community of the Lohit District of Arunachal Pradesh.
In the present study, we analyzed genetic variability and relatedness in 62 Gladiolus cultivars u... more In the present study, we analyzed genetic variability and relatedness in 62 Gladiolus cultivars using directed amplification of minisatellite DNA (DAMD) and inter simple sequence repeats (ISSR). The maximum inter-cultivar average genetic distance was 0.46 between Tiger Flame and Snow Flower cultivars, while the corresponding least genetic distance 0.14 was between Friendship Pink and Friendship White cultivars, respectively. The cumulative analysis carried out for the data generated with DAMD and ISSR markers showed 83.32% polymorphism across all the Gladiolus cultivars. This level of polymorphism resulted in the present investigation revealed that the amount of genetic variability in the Gladiolus genome is relatively high. The Jaccard’s similarity coefficient and clustering of genotypes in the unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed that some of the cultivars are closely related whereas others were found quite distinct from each other. The UPG...
Here we present a continuous palaeoclimatic record of 5980 years (7230 cal BP–1250 cal BP) from H... more Here we present a continuous palaeoclimatic record of 5980 years (7230 cal BP–1250 cal BP) from Hetapatti, a Neolithic site situated on the western fringe of the Central Ganga Plain. The region was a center of reurbanisation following the decline of the Harappan civilisation and is considered a hub of economic, political and religious evolution since the sixth millennium BC. Hetapatti contains an uninterrupted sequence from Neolithic to Historic (Gupta) period. The study integrates two different approaches (phytoliths and carbon isotope analysis) to infer vegetational and climatic changes and to understand their relationship to the cultural sequence. Our results show a gradual transformation from a warm and humid climate into a warm and dry climate from the Neolithic to Historic (Gupta) period. We find an abrupt weakening of the ISM at ~2080 cal BP driving a warm and dry climate. The comparison of our data with other high resolution regional and global records led us to hypothesise ...
Positron emission tomography (PET) imaging of the translocator 18 kDa protein (TSPO) with radioli... more Positron emission tomography (PET) imaging of the translocator 18 kDa protein (TSPO) with radioligands has become an effective means of research in peripheral inflammatory conditions that occur in many diseases and cancers. The peripheral sterile inflammatory diseases (PSIDs) are associated with a diverse group of disorders that comprises numerous enduring insults including the cardiovascular, respiratory, gastrointestinal, or musculoskeletal system. TSPO has recently been introduced as a potential biomarker for peripheral sterile inflammatory diseases (PSIDs). The major critical issue related to PSIDs is its timely characterization and localization of inflammatory foci for proper therapy of patients. As an alternative to metabolic imaging, protein imaging expressed on immune cells after activation is of great importance. The five transmembrane domain translocator protein-18 kDa (TSPO) is upregulated on the mitochondrial cell surface of macrophages during inflammation, serving as a potential ligand for PET tracers. Additionally, the overexpressed TSPO protein has been positively correlated with various tumor malignancies. In view of the association of escalated TSPO expression in both disease conditions, it is an immensely important biomarker for PET imaging in oncology and PSIDs. In this review, we summarize the most outstanding advances on TSPO-targeted PSIDs and cancer in the development of TSPO ligands as a potential diagnostic tool, specifically discussing the last five years.
We present the first systematic evaluation of the relationship between the archaeological and pal... more We present the first systematic evaluation of the relationship between the archaeological and palaeoclimatic record from north-western India during the past millennium, from the urban site of Chandravati. The rarity of Medieval sites, systematic excavations and multi-disciplinary work in the subcontinent obscure the impact of two distinct climate anomalies − the ‘Medieval Warm Period’ (‘MWP’, 740 − 1150 CE), followed by the ‘Little Ice Age’ (‘LIA’, 1350 − 1850 CE). The finds from the archaeological site indicate the presence of winter and summer crops, suggesting the region was likely warm and mild humid during pre-Medieval period (ca. 600 − 800 CE). During Medieval times (between ca. 800 − 1300 CE), a diversification of the crop assemblage suggests that the region was under a warm and humid climate, corresponding to the ‘MWP’, driving increased monsoon precipitation. During the post-Medieval period (ca. 1350 − 1800 CE), drought-resistant millets and other summer pulse crops indicat...
The plants of the genus Sapindus L. (Sapindaceae) are commercially important and are commonly kno... more The plants of the genus Sapindus L. (Sapindaceae) are commercially important and are commonly known as soap nut found both wild and introduced, in the tropical and sub-tropical regions of the world, chiefly in the Indo-Malayan region. Three species viz., S. emarginatus Vahl, S. mukorossi Gaertn. and S. trifoliatus L., occur in India. Of the Indian species S. mukorossi is commercially much valued for its fruits, that contains the saponins in the pericarp and are largely used in industrial and medicinal applications. In India S. mukorossi is found in Western Himalayan and North-East regions. In the present study ISSR markers were used to study genetic diversity in genotypes of the S. mukorossi in Western Himalaya. A total 54 genotypes of S. mukorossi representing two regions of Western Himalaya and one accessions of Lepisanthes tetraphylla Vahl., used as the out-group were taken into consideration. A total of 141 bands were obtained with 12 ISSR primers. Out of these, 88 bands were po...
Maize is an important crop in the North-Western Himalayan states of India for food, feed and nutr... more Maize is an important crop in the North-Western Himalayan states of India for food, feed and nutritional security of human population. Hybrid maize constitutes the major part of the maize area. Twenty four maize lines including the indigenous and exotic inbreds were amplified using 68 SSR primers, spread over the whole genome. The number of alleles across the primers ranged from two to eleven. The genotypes were grouped into different clusters using NTSYSpc2.11 programme. The clusters were well correlated with agronomic traits and resistance against turcicum blight. The PIC value was found to be highest for the primer bnlg1267 (0.84) while the lowest value was for the primer dupssr14 (0.09) with the mean value of 0.60. From this study we concluded that inbred V 359 is expected to give better combinations with CM 128, CM 129, V 340, V 357 and CM 212 for the development of hybrids suitable for the sub-tropical hill regions of India and elsewhere.
Genetic variability and population structure of Bergenia ciliata (Haw.) Sternb., commonly known a... more Genetic variability and population structure of Bergenia ciliata (Haw.) Sternb., commonly known as “ Pashanbheda ” (Stone-breaker), collected from the Western Himalayan region of India were estimated using two DNA fingerprinting methods viz ., directed amplification of minisatellite DNA (DAMD) and inter simple sequence repeats (ISSR). The cumulative data analysis of DAMD and ISSR markers for 74 accessions from eight populations showed 86.1% polymorphism. Analysis of molecular variance (AMOVA) showed highest percentage of variation within individuals of populations (73.6%) and 21.7% among populations. STRUCTURE and PCoA analyses on the hierarchical partitioning of genetic diversity showed strong admixture of individuals among the eight assumed geographical populations of B. ciliata . The data suggests that high genetic flow is one of the major factors responsible for low genetic differentiation. Preservation of genetic diversity of B. ciliata is important, both to promote adaptabilit...
Indian Journal of Natural Products and Resources, 2017
Other than the aesthetic values, orchids, the largest and most diverse group of flowering plants ... more Other than the aesthetic values, orchids, the largest and most diverse group of flowering plants has a broad range of ethnobotanical applications. However, little work has been done on ethnobotanical studies of orchids used by the Khamti community of the North-East India. The present report is an account of the ethnobotanical uses of 4 medicinal orchids, viz., Cymbidiun aloifolium (Linn.) Sw., Cymbidium bicolor Lindl., Eria pannea Lindl., and Dendrobium fimbriatum Hook. among the Khamti community of the Lohit District of Arunachal Pradesh.
In the present study, we analyzed genetic variability and relatedness in 62 Gladiolus cultivars u... more In the present study, we analyzed genetic variability and relatedness in 62 Gladiolus cultivars using directed amplification of minisatellite DNA (DAMD) and inter simple sequence repeats (ISSR). The maximum inter-cultivar average genetic distance was 0.46 between Tiger Flame and Snow Flower cultivars, while the corresponding least genetic distance 0.14 was between Friendship Pink and Friendship White cultivars, respectively. The cumulative analysis carried out for the data generated with DAMD and ISSR markers showed 83.32% polymorphism across all the Gladiolus cultivars. This level of polymorphism resulted in the present investigation revealed that the amount of genetic variability in the Gladiolus genome is relatively high. The Jaccard’s similarity coefficient and clustering of genotypes in the unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed that some of the cultivars are closely related whereas others were found quite distinct from each other. The UPG...
Here we present a continuous palaeoclimatic record of 5980 years (7230 cal BP–1250 cal BP) from H... more Here we present a continuous palaeoclimatic record of 5980 years (7230 cal BP–1250 cal BP) from Hetapatti, a Neolithic site situated on the western fringe of the Central Ganga Plain. The region was a center of reurbanisation following the decline of the Harappan civilisation and is considered a hub of economic, political and religious evolution since the sixth millennium BC. Hetapatti contains an uninterrupted sequence from Neolithic to Historic (Gupta) period. The study integrates two different approaches (phytoliths and carbon isotope analysis) to infer vegetational and climatic changes and to understand their relationship to the cultural sequence. Our results show a gradual transformation from a warm and humid climate into a warm and dry climate from the Neolithic to Historic (Gupta) period. We find an abrupt weakening of the ISM at ~2080 cal BP driving a warm and dry climate. The comparison of our data with other high resolution regional and global records led us to hypothesise ...
Positron emission tomography (PET) imaging of the translocator 18 kDa protein (TSPO) with radioli... more Positron emission tomography (PET) imaging of the translocator 18 kDa protein (TSPO) with radioligands has become an effective means of research in peripheral inflammatory conditions that occur in many diseases and cancers. The peripheral sterile inflammatory diseases (PSIDs) are associated with a diverse group of disorders that comprises numerous enduring insults including the cardiovascular, respiratory, gastrointestinal, or musculoskeletal system. TSPO has recently been introduced as a potential biomarker for peripheral sterile inflammatory diseases (PSIDs). The major critical issue related to PSIDs is its timely characterization and localization of inflammatory foci for proper therapy of patients. As an alternative to metabolic imaging, protein imaging expressed on immune cells after activation is of great importance. The five transmembrane domain translocator protein-18 kDa (TSPO) is upregulated on the mitochondrial cell surface of macrophages during inflammation, serving as a potential ligand for PET tracers. Additionally, the overexpressed TSPO protein has been positively correlated with various tumor malignancies. In view of the association of escalated TSPO expression in both disease conditions, it is an immensely important biomarker for PET imaging in oncology and PSIDs. In this review, we summarize the most outstanding advances on TSPO-targeted PSIDs and cancer in the development of TSPO ligands as a potential diagnostic tool, specifically discussing the last five years.
We present the first systematic evaluation of the relationship between the archaeological and pal... more We present the first systematic evaluation of the relationship between the archaeological and palaeoclimatic record from north-western India during the past millennium, from the urban site of Chandravati. The rarity of Medieval sites, systematic excavations and multi-disciplinary work in the subcontinent obscure the impact of two distinct climate anomalies − the ‘Medieval Warm Period’ (‘MWP’, 740 − 1150 CE), followed by the ‘Little Ice Age’ (‘LIA’, 1350 − 1850 CE). The finds from the archaeological site indicate the presence of winter and summer crops, suggesting the region was likely warm and mild humid during pre-Medieval period (ca. 600 − 800 CE). During Medieval times (between ca. 800 − 1300 CE), a diversification of the crop assemblage suggests that the region was under a warm and humid climate, corresponding to the ‘MWP’, driving increased monsoon precipitation. During the post-Medieval period (ca. 1350 − 1800 CE), drought-resistant millets and other summer pulse crops indicat...
The plants of the genus Sapindus L. (Sapindaceae) are commercially important and are commonly kno... more The plants of the genus Sapindus L. (Sapindaceae) are commercially important and are commonly known as soap nut found both wild and introduced, in the tropical and sub-tropical regions of the world, chiefly in the Indo-Malayan region. Three species viz., S. emarginatus Vahl, S. mukorossi Gaertn. and S. trifoliatus L., occur in India. Of the Indian species S. mukorossi is commercially much valued for its fruits, that contains the saponins in the pericarp and are largely used in industrial and medicinal applications. In India S. mukorossi is found in Western Himalayan and North-East regions. In the present study ISSR markers were used to study genetic diversity in genotypes of the S. mukorossi in Western Himalaya. A total 54 genotypes of S. mukorossi representing two regions of Western Himalaya and one accessions of Lepisanthes tetraphylla Vahl., used as the out-group were taken into consideration. A total of 141 bands were obtained with 12 ISSR primers. Out of these, 88 bands were po...
Maize is an important crop in the North-Western Himalayan states of India for food, feed and nutr... more Maize is an important crop in the North-Western Himalayan states of India for food, feed and nutritional security of human population. Hybrid maize constitutes the major part of the maize area. Twenty four maize lines including the indigenous and exotic inbreds were amplified using 68 SSR primers, spread over the whole genome. The number of alleles across the primers ranged from two to eleven. The genotypes were grouped into different clusters using NTSYSpc2.11 programme. The clusters were well correlated with agronomic traits and resistance against turcicum blight. The PIC value was found to be highest for the primer bnlg1267 (0.84) while the lowest value was for the primer dupssr14 (0.09) with the mean value of 0.60. From this study we concluded that inbred V 359 is expected to give better combinations with CM 128, CM 129, V 340, V 357 and CM 212 for the development of hybrids suitable for the sub-tropical hill regions of India and elsewhere.
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