Abstract
Confocal microscopy, which is a major advance upon normal light microscopy, has been used in a number of scientific fields. By confocal microscopy techniques, cells and tissues can be visualized deeply, and three-dimensional images created. Compared with conventional microscopes, confocal microscope improves the resolution of images by eliminating out-of-focus light. Moreover, confocal microscope has a higher level of sensitivity due to highly sensitive light detectors and the ability to accumulate images captured over time. In present studies, a series of Weeping Forsythia pollen digital images (35 images in total) were acquired with confocal microscope, and the three-dimensional digital image of the pollen reconstructed with confocal microscope. Our results indicate that it's a very easy job to analysis threedimensional digital image of the pollen with confocal microscope and the probe Acridine orange (AO).
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Liu, D., Chen, Z., Xu, H., Liu, W., Wang, L. (2009). RECONSTRUCTION OF 3D DIGITAL IMAGE OF WEEPINGFORSYTHIA POLLEN. In: Li, D., Zhao, C. (eds) Computer and Computing Technologies in Agriculture II, Volume 1. CCTA 2008. IFIP Advances in Information and Communication Technology, vol 293. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-0209-2_35
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DOI: https://doi.org/10.1007/978-1-4419-0209-2_35
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