Abstract
To investigate the neural mechanisms of insect locomotion, we aimed to generate genetic reagents to manipulate the specific cell types in the thoracic ganglion of Drosophila melanogaster. By using the split-Gal4 technique, we have generated ~1000 lines which can drive reporters or modulators in the specific cell types. We used these split-GAL4 lines to analyze the anatomy of individual cell types. Furthermore, we have screened their functions in locomotion by optogenetic activation. We observed a wide range of activation phenotypes, including the initiation and stopping of locomotion. These genetic resources will enable us to analyze the anatomy and function of insect locomotor circuits at the cellular level.
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Minegishi, R., Feng, K., Dickson, B. (2019). Cellular Level Analysis of the Locomotor Neural Circuits in Drosophila melanogaster. In: Martinez-Hernandez, U., et al. Biomimetic and Biohybrid Systems. Living Machines 2019. Lecture Notes in Computer Science(), vol 11556. Springer, Cham. https://doi.org/10.1007/978-3-030-24741-6_32
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DOI: https://doi.org/10.1007/978-3-030-24741-6_32
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