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Three-Dimensional Image Reconstruction of Murine Heart Using Image Processing

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Proceedings of 2021 International Conference on Medical Imaging and Computer-Aided Diagnosis (MICAD 2021) (MICAD 2021)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 784))

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Abstract

The key role of three-dimensional reconstructions in the analyses of medical imagery has gained more recognition over the past 20 years through many fields such as computer graphics and biological medicine. Specifically, lighting the role of isolated discrete mammalian cardiac tissues or organs typically involves a more accurate anatomical reconstruction procedure. To date, however, there has been no unified approach that could be extended to model establishments. This article seeks to amend these problems by introducing a new approach for studying the three-dimensional distribution of Pnmt+ cell-derived cells in isolated mouse hearts. Related data comes from Scientific Data that describes a new cardiomyocyte population which is a specific class of phenylethanolamine n-methyltransferase (Pnmt+) cell-derived cardiomyocytes (PdCMs). Rigid registration was implemented to match the raw sliced images of the murine heart using TrakEM2. Compared to previous reconstruction approaches, our methods have accomplished automated 3D reconstruction using image processing. The primary purpose of this paper is to propose an automatic image processing pipeline to recreate the 3D image of the murine heart, which prevents cell distribution distortion induced by handcrafted noise removal. The final 3D reconstructed exhibition was displayed by Paraview.

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References

  1. Camelliti, P., Abou Al-Saud, S., Smolenski, R.T., et al.: Adult human heart slices are a multicellular system suitable for electrophysiological and pharmacological studies. J. Mol. Cell. Cardiol. 51(3), 390–398 (2011)

    Article  Google Scholar 

  2. Lin, W., Dong, L.: Adaptive downsampling to improve image compression at low bit rates. IEEE Trans. Image Process. 15(9), 2513–2521 (2006)

    Article  Google Scholar 

  3. Ni, H., et al.: Three-dimensional image reconstruction of distribution of Pnmt + cell-derived cells in murine heart. Sci. Data 4, 170134 (2017). https://doi.org/10.1038/sdata.2017.134

    Article  Google Scholar 

  4. Wang, Y., et al.: Optogenetic control of heart rhythm by selective stimulation of cardiomyocytes derived from Pnmt+ cells in murine heart. Sci. Rep. 7, 40687 (2017). https://doi.org/10.1038/srep40687

    Article  Google Scholar 

  5. Ruggiero, D.A., Ross, C.A., Anwar, M., et al.: Distribution of neurons containing phenylethanolamine N-methyltransferase in medulla and hypothalamus of rat. J. Comp. Neurol. 239(2), 127–154 (1985)

    Article  Google Scholar 

  6. Deisseroth, K.: Optogenetics. Nat. Methods 8(1), 26–29 (2011). https://doi.org/10.1038/nmeth.f.324

    Article  Google Scholar 

  7. Soulez, F., Denis, L., Tourneur, Y., et al.: Blind deconvolution of 3D data in wide field fluorescence microscopy. In: 2012 9th IEEE International Symposium on Biomedical Imaging (ISBI). Pp. 1735–1738. IEEE (2012)

    Google Scholar 

  8. SoftWoRx G E. Healthcare. http://incelldownload.gehealthcare.com/bin/download_data/so-ftWoRx/7.00.SoftWoRx.htm

  9. Abràmoff, M.D., Magalhães, P.J., Ram, S.J.: Image processing with ImageJ. Biophoton. Int. 11(7), 36–42 (2004)

    Google Scholar 

  10. Cardona, A.: TrakEM2: an ImageJ-based program for morphological data mining and 3D modelling. In: Proceedings of the Image J User and Developer Conference (2006)

    Google Scholar 

  11. Schindelin, J., Arganda-Carreras, I., Frise, E., et al.: Fiji: an open-source platform for biological-image analysis. Nat. Methods 9(7), 676–682 (2012)

    Article  Google Scholar 

  12. Higham, D.J., Higham, N.J.: MATLAB guide. Society for Industrial and Applied Mathematics (2016)

    Google Scholar 

  13. Justusson, B.I.: Median filtering: Statistical properties. In: Justusson, B.I. (ed.) Two-Dimensional Digital Signal Prcessing II, 161–196. Springer, Heidelberg (1981). https://doi.org/10.1007/BFb0057597

    Chapter  Google Scholar 

  14. Schroeder, W.J., Avila, L.S., Hoffman, W.: Visualizing with VTK: a tutorial. IEEE Comput. Graph. Appl. 20(5), 20–27 (2000)

    Article  Google Scholar 

  15. Ayachit, U.: The Paraview Guide: A Parallel Visualization Application. Kitware, Inc. (2015)

    Google Scholar 

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Zhong, H., Huang, C., Cui, J., Hu, W. (2022). Three-Dimensional Image Reconstruction of Murine Heart Using Image Processing. In: Su, R., Zhang, YD., Liu, H. (eds) Proceedings of 2021 International Conference on Medical Imaging and Computer-Aided Diagnosis (MICAD 2021). MICAD 2021. Lecture Notes in Electrical Engineering, vol 784. Springer, Singapore. https://doi.org/10.1007/978-981-16-3880-0_2

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  • DOI: https://doi.org/10.1007/978-981-16-3880-0_2

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-16-3879-4

  • Online ISBN: 978-981-16-3880-0

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