Summary
Fragmentation patterns of the essential amino acids (AAs) as their silyl derivatives have been obtained with the aid of ion trap detection (ITD). Three derivatizing reagents, hexamethyldisilazane+trifluoroacetic acid (HMDS+TFAA),bis-(trimethylsilyl)trifluoroacetamide (BSTFA) andN-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide (MTBSTFA) were used. Simple and multiple derivatives obtained with each reagent have been investigated, with regard to their sensitivity and selectivity.
Our study performed in the concentration range of 5-2000 ng amino acids has shown that, contrary to literature data, thirteen of the twenty-two AAs investigated including the TBDMS derivatives give rise to more than one peak when eluted. As a result of ion/molecule interaction the very informative ions of high masses, ([M]+, [M+TMS/(TBDMS)]+, [M+1]+) are formed with considerable intensities. The fragments [M-CH3]+, [M-C4H9]+, [M-(CH3)2Si]+, [M-TMS/(TBDMS)COO]+, [M-TBDMSOH]+, [M-TBDMSO]+, [M-TBDMSNH]+ and numerous others could be utilized for identification purposes.
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Molnár-Perl, I., Katona, Z.F. GC-MS of amino acids as their trimethylsilyl/t-butyldimethylsilyl Derivatives: In model solutions III. Chromatographia 51, S228–S236 (2000). https://doi.org/10.1007/BF02492811
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DOI: https://doi.org/10.1007/BF02492811