Effects of cultivating conditions on the mycelial growth of Ganoderma lucidum in submerged flask cultures

Abstract

In this paper the effects of environmental conditions on the mycelial growth of Ganoderma lucidum were investigated in shake flask cultures. The optimal temperature and pH were found to be around 30–35 °C and 4, respectively, in a glucose-ammonium chloride medium. The maximum mycelial concentration reached to around 350 mg/100 ml. The formation of mycelial pellets and their ultra structure was demonstrated to be greatly affected by cultivating conditions. Increasing surface aeration would be beneficial for mycelial growth. However, too high rotating speed in shake cultures had a detrimental effect on the formation of mycelial pellets and the optimum was found to be 100 rpm.