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Enzymatic activity of vibrios isolated from the hemolymph of cultured Litopenaeus vannamei shrimp

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Abstract

The enzymatic characterization of 100 strains of Vibrio isolated from the hemolymph of Litopenaeus vannamei was performed. All the strains were subjected to caseinase (Cas), gelatinase (Gel), elastase (Elas), phospholipase (Phos), lipase (Lip) and β-hemolysis detection. The enzymatic profile indicated the following positivity indices: 96 % of the isolates were Cas+, 80 % Gel+, 35 % Elas+, 94 % Phos+, 58 % Lip+, and 38 % expressed the ability to produce β-hemolysis in sheep erythrocytes. Coincidental exoenzymatic expression was observed in 99 % of the isolates, and the profiles GelCasPhos (16 %) and GelCasPhosLip (16 %) were the most frequent. Additional enzymatic activity experiments in 24 strains showed a positivity index higher than 90 % for esterase (C4), leucine arylamidase, acid phosphatase and alkaline phosphatase.

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Acknowledgments

The first author received a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Thanks are due to Jorge L Adeodato Júnior for help with the English translation.

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Authors and Affiliations

  1. Sea Science Institute, Federal University of Ceará, Abolição Av. 3207, Fortaleza, Ceará, 60165-081, Brazil

    Renata Albuquerque Costa, Rayza Lima Araújo & Regine Helena Silva dos Fernandes Vieira

  2. Engineering Fishing Department, Campus do Pici, Federal University of Ceará, blocks 825, 827 and 840, Fortaleza, Ceará, 60356-000, Brazil

    Renata Albuquerque Costa, Rayza Lima Araújo & Regine Helena Silva dos Fernandes Vieira

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  1. Renata Albuquerque Costa
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  2. Rayza Lima Araújo
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  3. Regine Helena Silva dos Fernandes Vieira
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Correspondence to Renata Albuquerque Costa.

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Costa, R.A., Araújo, R.L. & dos Fernandes Vieira, R.H.S. Enzymatic activity of vibrios isolated from the hemolymph of cultured Litopenaeus vannamei shrimp. J. Verbr. Lebensm. 8, 307–311 (2013). https://doi.org/10.1007/s00003-013-0841-0

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  • DOI: https://doi.org/10.1007/s00003-013-0841-0

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