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Redox electrodeposition polymers: adaptation of the redox potential of polymer-bound Os complexes for bioanalytical applications

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Abstract

The design of polymers carrying suitable ligands for coordinating Os complexes in ligand exchange reactions against labile chloro ligands is a strategy for the synthesis of redox polymers with bound Os centers which exhibit a wide variation in their redox potential. This strategy is applied to polymers with an additional variation of the properties of the polymer backbone with respect to pH-dependent solubility, monomer composition, hydrophilicity etc. A library of Os-complex-modified electrodeposition polymers was synthesized and initially tested with respect to their electron-transfer ability in combination with enzymes such as glucose oxidase, cellobiose dehydrogenase, and PQQ-dependent glucose dehydrogenase entrapped during the pH-induced deposition process. The different polymer-bound Os complexes in a library containing 50 different redox polymers allowed the statistical evaluation of the impact of an individual ligand to the overall redox potential of an Os complex. Using a simple linear regression algorithm prediction of the redox potential of Os complexes becomes feasible. Thus, a redox polymer can now be designed to optimally interact in electron-transfer reactions with a selected enzyme.

A library of redox electrodeposition polymers was synthesized and the formal potentials of the polymer-bound Os-complexes were adjusted through variations of the coordination shell. Optimal adaptation to the redox potentials of enzymes could be attained

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Acknowledgments

The authors are grateful to the European Commission in the framework of the project “3D-Nanobiodevice” (NMP4-SL-2009-229255) and to the German Science Foundation in the framework of the ERA-Chemistry Open Initiative 2008 (DFG; SCHU 929/10-1) for financial support of part of the work.

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  1. Analytische Chemie—Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany

    Dmitrii A. Guschin, John Castillo, Nina Dimcheva & Wolfgang Schuhmann

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  1. Dmitrii A. Guschin
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  4. Wolfgang Schuhmann
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Correspondence to Wolfgang Schuhmann.

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Guschin, D.A., Castillo, J., Dimcheva, N. et al. Redox electrodeposition polymers: adaptation of the redox potential of polymer-bound Os complexes for bioanalytical applications. Anal Bioanal Chem 398, 1661–1673 (2010). https://doi.org/10.1007/s00216-010-3982-3

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  • DOI: https://doi.org/10.1007/s00216-010-3982-3

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