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Analysis of methanol–ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase

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Abstract

A portable bienzymatic analytical system was developed for the chronoamperometric analysis of methanol–ethanol mixtures. The system consists of two biosensors, one based on alcohol dehydrogenase (ADH) that responds only to the ethanol and the second one based on alcohol oxidase (AOX) that responds to both methanol and ethanol. The transducers were screen-printed electrodes (SPEs) modified with mediators: Meldola blue for ADH and Co-phthalocyanine for AOX. The calibration graph of the ADH biosensor is linear between 0.3 and 8 mmol/L ethanol. The AOX biosensor is able to quantify both analytes in mixtures that contain methanol between 3 and 70 mmol/L and ethanol ranging from 15 to 110 mmol/L. Interferences due to non-specific oxidations from common oxidizable compounds like gallic acid and ascorbic acid were smaller in the case of transducer based on Meldola blue. The analytical system was successfully tested on real samples: non-alcoholised beer (NAB) spiked with ethanol or methanol and a falsified rose wine (FRW).

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Abbreviations

ADH:

Alcohol dehydrogenase

AOX:

Alcohol oxidase

SPEs:

Screen-printed electrodes

WE:

Working electrode

NAD+/NADH:

Nicotinamide adenine dinucleotide oxidized/reduced forms

FAD:

Flavin adenine dinucleotide

K M :

Michaelis constant

PVA-SbQ:

Polyvinyl alcohol containing stilbazolium groups

NAB:

Non-alcoholic beer

FRW:

Falsified rose wine

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Acknowledgements

Bucur Bogdan is a post-doctoral fellow of Romanian Ministry of Education and Research through Grant PD-011/2005. The work was supported by Romanian Ministry of Education and Research through Grant CEEX-156/2006. The Meldola blue modified screen-printed paste was a gift from Gwent Electronic Materials (UK). The authors wish to thank Prof. Jean-Louis Marty (BIOMEM-University of Perpignan, France) for the SPEs and PVA polymer and Prof. Didier Fournier (Paul Sabatier University, Toulouse, France) for helpful discussions on enzymatic kinetic.

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Authors and Affiliations

  1. Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7, Polizu, Bucharest, 011061, Romania

    Bogdan Bucur

  2. Centre of Bioanalysis, National Institute of Biological Sciences, 296, Splaiul Independentei, Bucharest, 060031, Romania

    Gabriel Lucian Radu

  3. Scientifical Research Center for Defence NBC and Ecology, Sos. Oltenitei, nr.225, Sector 4, Bucharest, Romania

    Constantin Nicolae Toader

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  1. Bogdan Bucur
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  2. Gabriel Lucian Radu
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  3. Constantin Nicolae Toader
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Correspondence to Bogdan Bucur.

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Bucur, B., Radu, G.L. & Toader, C.N. Analysis of methanol–ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase. Eur Food Res Technol 226, 1335–1342 (2008). https://doi.org/10.1007/s00217-007-0662-4

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  • DOI: https://doi.org/10.1007/s00217-007-0662-4

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