Abstract
Cold-inducible RNA-binding protein (CIRBP) responds to a wide array of cellular stresses such as cold shock, hypoxia, and inflammatory responses. However, functional studies of CIRBP in jawless vertebrates are limited. In this study, a CIRBP homolog from the jawless vertebrate lamprey (Lethenteron reissneri) was cloned and characterized (named Lr-CIRBP). The cDNA fragment of Lr-CIRBP has a 516 bp open reading frame (ORF) that encodes 171 amino acids, comprising a glycine-rich region at the C-terminal, similar to higher vertebrates but slightly shorter, and an RNA recognition motif (RRM) domain at the N-terminus. The predicted Lr-CIRBP sequence had 51.4 ~ 70.6% similarity with CIRBPs from other vertebrates. Further phylogenetic analysis revealed that Lr-CIRBP is located in the outgroup of vertebrates and is the ancestor of vertebrates. Based on real-time quantitative PCR experimental analysis, Lr-CIRBP expression was highest in leukocytes and increased significantly after multi-stimulation, peaking at 12 h. RNA interference showed that Lr-CIRBP knockdown can down-regulate the expression of inflammatory factors in Lethenteron reissneri. In conclusion, our study successfully clarifies the ancestral features and functions of CIRBP, while revealing valuable insight into how the protein is involved in the immune responses of a jawless vertebrate.
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Data availability
All relevant data are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors thank Jiao Tong of MEDx Co., Ltd for her help with this research.
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This work was funded by the High-level Talent Innovation Support Program of Dalian (2017RQ046), the Young Star of Science and Technology of Dalian in 2020 (Grant No.2020RQ072), and the Youth Fund of Liaoning Provincial Department of Education (LQ2019025).
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All animal experiments were approved by the Animal Welfare and Research Ethics Committee of the Institute of Dalian Medical University and operated following the Guide for the Care and Use of Laboratory Animals 8th Edition Written by the National Research Council.
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Communicated by Matthias Hammerschmidt
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Duan, X., Pan, T., Wang, Z. et al. Molecular characterization and expression analysis of a novel cold-inducible RNA-binding protein (CIRBP) gene in lamprey (Lethenteron reissneri). Dev Genes Evol 233, 49–57 (2023). https://doi.org/10.1007/s00427-022-00700-8
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DOI: https://doi.org/10.1007/s00427-022-00700-8