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CRISPR-Cpf1 system and its applications in animal genome editing

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Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein (Cas) system is a gene editing technology guided by RNA endonuclease. The CRISPR-Cas12a (also known as CRISPR-Cpf1) system is extensively utilized in genome editing research due to its accuracy and high efficiency. In this paper, we primarily focus on the application of CRISPR-Cpf1 technology in the construction of disease models and gene therapy. Firstly, the structure and mechanism of the CRISPR-Cas system are introduced. Secondly, the similarities and differences between CRISPR-Cpf1 and CRISPR-Cas9 technologies are compared. Thirdly, the main focus is on the application of the CRISPR-Cpf1 system in cell and animal genome editing. Finally, the challenges faced by CRISPR-Cpf1 technology and corresponding strategies are analyzed. Although CRISPR-Cpf1 technology has certain off-target effects, it can effectively and accurately edit cell and animal genomes, and has significant advantages in the preclinical research.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 81801127), Key Technologies R&D Program of Henan Province (No. 242102310134), Doctoral Scientific Research Program Foundation of Xinxiang Medical University (No. XYBSKYZZ201523).

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Authors and Affiliations

  1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, Henan, China

    Yawei Han

  2. Stem Cells and Biotherapy Engineering Research Center of Henan, National Joint Engineering Laboratory of Stem Cells and Biotherapy, School of Life Science and Technology, Xinxiang Medical University, Number 601, Jinsui Road, Xinxiang, 453003, Henan, China

    Zisen Jia, Keli Xu, Yangyang Li & Lihong Guan

  3. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei, China

    Suxiang Lu

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  1. Yawei Han
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  2. Zisen Jia
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  3. Keli Xu
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  4. Yangyang Li
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  5. Suxiang Lu
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  6. Lihong Guan
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Contributions

YH and ZJ: Investigation, Conceptualization, Figure production, Writing - review & editing. KX and YL: Investigation, Figure production and Writing. SL: Figure production. LG: Supervision, Conceptualization, Funding acquisition, Writing - review & editing. All the authors read and approved the manuscript.

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Correspondence to Lihong Guan.

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Communicated by Martine Collart.

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Han, Y., Jia, Z., Xu, K. et al. CRISPR-Cpf1 system and its applications in animal genome editing. Mol Genet Genomics 299, 75 (2024). https://doi.org/10.1007/s00438-024-02166-x

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