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Current achievements in the production of complex biopharmaceuticals with moss bioreactors

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Abstract

Transgenic plants are promising alternatives for the low-cost and safe pathogen-free production of complex recombinant pharmaceutical proteins (molecular farming). Plants as higher eukaryotes perform posttranslational modifications similar to those of mammalian cells. However, plant-specific protein N-glycosylation was shown to be immunogenic, a fact that represents a drawback for many plant systems in biopharmaceutical production. The moss Physcomitrella patens offers unique properties as a contained system for protein production. It is grown in the predominant haploid gametophytic stage as tissue suspension cultures in photobioreactors. Efficient secretory signals and a transient transfection system allow the secretion of freshly synthesized proteins to the surrounding medium. The key advantage of Physcomitrella compared to other plant systems is the feasibility of targeted gene replacements. By this means, moss strains with non-immunogenic humanized glycan patterns were created. Here we present an overview of the relevant aspects for establishing moss as a production system for recombinant biopharmaceuticals.

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Abbreviations

GMP:

Good manufacturing practice

ADCC:

Antibody-dependent cellular cytotoxicity

HAS:

Human serum albumin

VEGF:

Vascular endothelial growth factor

CHO:

Chinese Hamster Ovary

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Acknowledgments

This work was supported by the German Federal Ministry of Education and Research (BMBF grants 0312624 and 0313852), the German Academic Exchange Service (DAAD), and the Wissenschaftliche Gesellschaft of the University of Freiburg.

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  1. Plant Biotechnology, Faculty of Biology, Freiburg University, Schaenzlestr. 1, 79104, Freiburg, Germany

    Eva L. Decker & Ralf Reski

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Correspondence to Eva L. Decker.

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Decker, E.L., Reski, R. Current achievements in the production of complex biopharmaceuticals with moss bioreactors. Bioprocess Biosyst Eng 31, 3–9 (2008). https://doi.org/10.1007/s00449-007-0151-y

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