Abstract
The one-step synthesis is reported of a nanofilm composed of iron oxide and gold nanoparticles in a chitosan matrix that can act as a novel matrix for the immobilization of glucose oxidase (GOx) to fabricate a glucose biosensor. The use for the composite film strongly increased the effective electrode surface for loading of GOx. The size and shape of the iron oxide nanoparticles were examined by transmission electron micrograph. Direct electron transfer and electrocatalysis by GOx was investigated via cyclic voltammetry and chronoamperometry. Under optimized conditions, the biosensor has a response time of 6 s and a linear response in the range between 3 μM and 0.57 mM of glucose, with a detection limit of 1.2 μM at a signal-to-noise ratio of 3. This novel and disposable mediatorless glucose biosensor may form the basis for a future mass-produced glucose biosensor.
In this paper, based on the direct electrochemistry of redox enzyme, we try to integrate the excellent properties of iron oxide-gold nanoparticle-chitosan composite film with the advantages of one-step electrodeposition to fabricate a sensitive and stable glucose biosensor.
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Acknowledgements
This work was supported by the NNSF of China (20675064), the Ministry of Education of China (Project 708073), the Natural Science Foundation of Chongqing City (CSTC-2009BA1003) and High Technology Project Foundation of Southwest University (XSGX02), China.
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Li, J., Yuan, R. & Chai, Y. Simple construction of an enzymatic glucose biosensor based on a nanocomposite film prepared in one step from iron oxide, gold nanoparticles, and chitosan. Microchim Acta 173, 369–374 (2011). https://doi.org/10.1007/s00604-011-0544-0
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DOI: https://doi.org/10.1007/s00604-011-0544-0