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Surface molecularly imprinted polydopamine films for recognition of immunoglobulin G

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Abstract

We have prepared a surface imprinted polymer (SIP) film for label-free recognition of immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by destruction of the cleavable bond so that complementary binding sites were created on the surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with respect to their affinity to IgG using a quartz crystal microbalance combined with flow injection analysis. The films were also characterized by cyclic voltammetry and scanning electron microscopy. The IgG-SIPs with a film thickness of around 17 nm showed the most pronounced imprinting effect (IF 1.66) and a binding constant of 296 nM.

A strategy for preparation of the IgG-Surface Imprinted Polymeric (IgG-SIP) thin films was developed. IgG was covalently immobilized via a cleavable cross-linker to a gold electrode surface followed by electrochemical deposition of a nanometer thin PDA film. After cleaving S-S bond in the linker the IgG was removed leaving behind the complementary binding sites confined in the surface of the polymer film. The prepared IgG-SIPs were applied for IgG recognition.

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Acknowledgements

This work has been supported by the Estonian Ministry of Education and Research (grant PUT150) and the Estonian Science Foundation (grant ETF8249).

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Authors and Affiliations

  1. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, 19086, Tallinn, Estonia

    Aleksei Tretjakov, Vitali Syritski, Jekaterina Reut, Roman Boroznjak, Olga Volobujeva & Andres Öpik

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  1. Aleksei Tretjakov
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  2. Vitali Syritski
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  3. Jekaterina Reut
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  4. Roman Boroznjak
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Correspondence to Vitali Syritski.

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Tretjakov, A., Syritski, V., Reut, J. et al. Surface molecularly imprinted polydopamine films for recognition of immunoglobulin G. Microchim Acta 180, 1433–1442 (2013). https://doi.org/10.1007/s00604-013-1039-y

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  • DOI: https://doi.org/10.1007/s00604-013-1039-y

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