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Use of gold nanoparticle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin B1

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Abstract

We describe a sensitive method for the immunochromatographic determination of aflatoxin B1. It is based on the following steps: 1) Competitive interaction between non-labeled specific primary antibodies and target antigens in a sample and in the test zone of a membrane; 2) detection of the immune complexes on the membrane by using a secondary antibodies labeled with gold nanoparticles. The method enables precise adjustment of the required quantities of specific antibodies and the colloidal (gold) marker. It was applied in a lateral flow format to the detection of aflatoxin B1 and exhibits a limit of detection (LOD) of 160 pg · mL−1 if detected visually, and of 30 pg · mL−1 via instrumental detection. This is significantly lower than the LOD of 2 ng · mL−1 achieved by conventional lateral flow analysis using the same reagents.

Immunochromatography with secondary labeled antibodies caused 10-fold decrease of detection limit

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Acknowledgments

This work was supported by the Russian Foundation for Basic Research (grants 10-03-00990, 11-08-93968, 12-08-01303, 13-04-90479) and the Federal Target Program “Scientific and scientific-pedagogical personnel of the innovative Russia” for 2009–2013 (agreement 8284).

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  1. A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia

    Alexander E. Urusov, Anatoly V. Zherdev & Boris B. Dzantiev

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  1. Alexander E. Urusov
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  2. Anatoly V. Zherdev
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  3. Boris B. Dzantiev
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Correspondence to Boris B. Dzantiev.

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Urusov, A.E., Zherdev, A.V. & Dzantiev, B.B. Use of gold nanoparticle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin B1. Microchim Acta 181, 1939–1946 (2014). https://doi.org/10.1007/s00604-014-1288-4

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  • DOI: https://doi.org/10.1007/s00604-014-1288-4

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