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Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress

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Abstract

In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by upregulating the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR). Drought stress also markedly increased electrolyte leakage (EL), malondialdehyde (MDA) content, NO content, and significantly reduced the ratios of reduced ascorbate/dehydroascorbic acid (AsA/DHA) and reduced glutathione/oxidized glutathione (GSH/GSSG) compared with control. Exogenous JA significantly increased the above indicators, compared with drought stress alone. All these effects of JA were inhibited by pretreatment with NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). Meanwhile, exogenous JA markedly decreased MDA content and electrolyte leakage of wheat leaves under drought stress. Pretreatment with cPTIO reversed the above effects of exogenous JA. Our findings indicated that NO induced by exogenous JA upregulated the activity of the AsA-GSH cycle and had important role in drought tolerance.

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Acknowledgments

Our study was funded by “The Starting Fund for High-level Talents (Doctor) of Henan Institute of Science and Technology (2011010)”.

Conflict of interest

There is no conflict of interest between the authors.

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Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, China

    Changjuan Shan, Yan Zhou & Mingjiu Liu

  2. Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang, 453003, China

    Changjuan Shan, Yan Zhou & Mingjiu Liu

Authors
  1. Changjuan Shan
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  2. Yan Zhou
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  3. Mingjiu Liu
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Correspondence to Changjuan Shan.

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Handling Editor: Néstor Carrillo

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Shan, C., Zhou, Y. & Liu, M. Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress. Protoplasma 252, 1397–1405 (2015). https://doi.org/10.1007/s00709-015-0756-y

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  • DOI: https://doi.org/10.1007/s00709-015-0756-y

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