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Biocompatibility and biomineralization assessment of mineral trioxide aggregate flow

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Abstract

Objective

Evaluate, in vivo, the biocompatibility via subcutaneous inflammatory tissue response and mineralization ability of the new MTA Flow compared to MTA Angelus and ProRoot MTA.

Materials and methods

Forty male Wistar rats were assigned and received subcutaneous polyethylene tube implants containing the test materials and a control group with empty tube (n = 10 animals/group). After days 7, 15, 30, and 60, the animals were euthanized and the polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and under polarized light. Data were analyzed via Kruskal-Wallis and Dunn’s test with a significance level of 5%.

Results

MTA Angelus induced the mildest reaction after 7 (P > .05) and 15 days (P < .05) followed by MTA Flow, both cements achieving mild inflammatory reaction after 15 days. ProRoot MTA induced a severe inflammation on day 7 and was reducing after day 15 (P > .05). No difference was observed after days 30 or 60 (P > .05). Von Kossa staining and birefringent structures were positive to all materials.

Conclusions

At the end of the experiment, the novel MTA Flow showed biocompatibility and induced biomineralization in all time periods.

Clinical relevance

The final consistence obtained in MTA Flow may facilitate several procedures, indicating that the MTA Flow has a promising application in endodontics.

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Acknowledgments

The authors would like to thank Ultradent Products, Inc. for providing the MTA Flow used in this study.

Funding

This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.

Author information

Authors and Affiliations

  1. Department of Endodontics, Araçatuba School of Dentistry, Sao Paulo State University (Unesp), R: José Bonifácio, 1193, Vila Mendonça, Araçatuba/São Paulo, 16015-050, Brazil

    Carlos Roberto Emerenciano Bueno, Ana Maria Veiga Vasques, Marina Tolomei Sandoval Cury, Gustavo Sivieri-Araújo, Rogério Castilho Jacinto, João Eduardo Gomes-Filho, Luciano Tavares Angelo Cintra & Eloi Dezan-Júnior

Authors
  1. Carlos Roberto Emerenciano Bueno
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  2. Ana Maria Veiga Vasques
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  3. Marina Tolomei Sandoval Cury
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  4. Gustavo Sivieri-Araújo
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  5. Rogério Castilho Jacinto
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  6. João Eduardo Gomes-Filho
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  7. Luciano Tavares Angelo Cintra
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  8. Eloi Dezan-Júnior
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Corresponding author

Correspondence to Eloi Dezan-Júnior.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Before any procedure, the project was approved by Faculdade de Odontologia de Araçatuba, UNESP, Animal Ethical Committee (CEUA protocol 00225-2017).

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For this type of study, formal consent is not required.

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Bueno, C.R.E., Vasques, A.M.V., Cury, M.T.S. et al. Biocompatibility and biomineralization assessment of mineral trioxide aggregate flow. Clin Oral Invest 23, 169–177 (2019). https://doi.org/10.1007/s00784-018-2423-0

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  • DOI: https://doi.org/10.1007/s00784-018-2423-0

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