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Development of chitosan-tripolyphosphate non-woven fibrous scaffolds for tissue engineering application

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Abstract

The fibrous scaffolds are promising for tissue engineering applications because of their close structural resemblance with native extracellular matrix. Additionally, the chemical composition of scaffold is also an important consideration as they have significant influences on modulating cell attachment, morphology and function. In this study, chitosan-tripolyphosphate (TPP) non-woven fibrous scaffolds were prepared through wetspinning process. Interestingly, at physiological pH these scaffolds release phosphate ions, which have significant influences on cellular function. For the first time, cell viability in presence of varying concentration of sodium TPP solution was analyzed and correlated with the phosphate release from the scaffolds during 30 days incubation period. In vitro degradation of the chitosan-TPP scaffolds was higher than chitosan scaffolds, which may be due to decrease in crystallinity as a result of instantaneous ionic cross-linking during fiber formation. The scaffolds with highly interconnected porous structure present a remarkable cytocompatibility for cell growing, and show a great potential for tissue engineering applications.

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Acknowledgments

Authors would like to acknowledge DBT and DST, Government of India for financial support and IIT Kharagpur for providing infrastructural facility. Finally, all lab members of Tissue Engineering laboratory at SMST, IIT Kharagpur are acknowledged for support.

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Authors and Affiliations

  1. School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, 721302, India

    Falguni Pati & Santanu Dhara

  2. Materials Science Centre, Indian Institute of Technology, Kharagpur, 721302, India

    Basudam Adhikari

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  1. Falguni Pati
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Correspondence to Santanu Dhara.

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Pati, F., Adhikari, B. & Dhara, S. Development of chitosan-tripolyphosphate non-woven fibrous scaffolds for tissue engineering application. J Mater Sci: Mater Med 23, 1085–1096 (2012). https://doi.org/10.1007/s10856-012-4559-9

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