Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Skip to main content

Advertisement

Springer Nature Link
Log in

Physiological introspection into differential drought tolerance in rice cultivars of North East India

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Drought is a vice to world crop production, exponentially enhanced by global climate change. Rice, a basic food crop for a major chunk of world populace, is largely affected by environmental challenges such as drought, salinity and heavy metal. This study brings to limelight differential drought tolerance capacity of rice varieties indigenous to North East India, a hot bed of indica rice diversity. Initial screening of rice varieties were performed through physiological dose-dependent studies under PEG (0%, 10%, and 20% which is equal to osmotic potential values of 0.001, 0.54 and 1.09 MPa, respectively), induced drought stress for three time intervals of 1, 3 and 5 days. Hierarchical clustering of the parameters on which the cultivars were analysed revealed Tampha and KMJ 1-12-3 to be relatively more tolerant whereas Chandan and Ketaki Joha as the sensitive ones. Biochemical studies for hydrogen peroxide (H2O2), proline, lipid peroxidation (MDA), lipoxygenase (LOX), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) were then performed on two cultivars (Tampha and Chandan) to elucidate the differential tolerance to drought, focusing on anti-oxidative response mechanism. The biochemical fingerprint showed significantly lower accumulation of H2O2 and MDA; higher accumulation of proline; and higher activity of SOD, CAT, DHAR, MDHAR and GR in the tolerant variety Tampha when compared to Chandan. In addition, alteration of chlorophyll fluorescence due to stress was also monitored to ascertain the variation in photosynthetic efficiency between the tolerant and sensitive cultivars. Tampha showed better photosynthetic activity in comparison to Chandan as quantified by measuring chlorophyll fluorescence. This manuscript thus throws new light into the drought stress response of the varieties from North East India with global implications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Ali MN, Yeasmin L, Gantait S, Goswami R, Chakraborty S (2014) Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers. Physiol Mol Biol Plants 20(4):411–423

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W (2011) Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res 6(9):2026–2032

    Google Scholar 

  • Annual Report IRRI (2016). https://irri.org/resources-and-tools/publications

  • Awasthi J, Saha B, Chowardhara B, Devi S, Borgohain P, Panda S (2018) Qualitative analysis of lipid peroxidation in plants under multiple stress through schiff’s reagent: a histochemical approach. Bioprotocol 8(8):e2807. https://doi.org/10.21769/BioProtoc.2807

    Article  Google Scholar 

  • Axelrod B, Cheesbrough TM, Laakso S (1981) Lipoxygenase from soybeans. Methods Enzymol 71:441–451

    Article  CAS  Google Scholar 

  • Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60(1):51

    Article  CAS  Google Scholar 

  • Bates LS, Walden RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207

    Article  CAS  Google Scholar 

  • Bhaskara GB, Wen TN, Nguyen TT, Verslues PE (2017) Protein phosphatase 2Cs and microtubule-associated stress protein 1 control microtubule stability, plant growth, and drought response. Plant Cell Online 29(1):169–191

    Article  CAS  Google Scholar 

  • Chance B, Maehly AC (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775

    Article  Google Scholar 

  • Cheng F, Lu J, Gao M, Shi K, Kong Q, Huang Y et al (2016) Redox signaling and CBF-responsive pathway are involved in salicylic acid-improved photosynthesis and growth under chilling stress in watermelon. Front Plant Sci 7:519. https://doi.org/10.3389/fpls.2016.01519

    Article  Google Scholar 

  • Cruz de Carvalho MH (2008) Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signal Behav 3(3):156–165

    Article  PubMed  PubMed Central  Google Scholar 

  • Das A, Ghosh PK, Lal R, Saha R, Ngachan S (2017) Soil quality effect of conservation practices in maize–rapeseed cropping system in Eastern Himalaya. Land Degrad Dev 28(6):1862–1874

    Article  Google Scholar 

  • Devi LJ (2014) Evaluation of some common rice varieties of Manipur for resistance against rice root-knot nematode. Eur J Biotechnol Biosci 1(3):39–41

    Google Scholar 

  • Fang Y, Xiong L (2015) General mechanisms of drought response and their application in drought resistance improvement in plants. Cell Mol Life Sci 72(4):673–689

    Article  CAS  PubMed  Google Scholar 

  • Foyer CH, Noctor G (2011) Ascorbate and glutathione: the heart of the redox hub. Plant Physiol 155(1):2–18

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59(2):309–314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930

    Article  CAS  PubMed  Google Scholar 

  • Griggs D, Stafford-Smith M, Gaffney O, Rockström J, Öhman MC, Shyamsundar P, Noble I (2013) Policy: sustainable development goals for people and planet. Nature 495(7441):305–307

    Article  CAS  PubMed  Google Scholar 

  • Hasanuzzaman M, Nahar K, Rahman A, Inafuku M, Oku H, Fujita M (2018) Exogenous nitric oxide donor and arginine provide protection against short-term drought stress in wheat seedlings. Physiol Mol Biol Plants 24:1–12

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Arch Biochem Biophys 125(1):189–198

    Article  CAS  PubMed  Google Scholar 

  • Hellal FA, El-Shabrawi HM, El-Hady MA, Khatab IA, El-Sayed SAA, Abdelly C (2018) Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. J Genet Eng Biotechnol 16(1):203–212

    Article  CAS  PubMed  Google Scholar 

  • Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Circ Calif Agric Exp Stn 347:1–39

    Google Scholar 

  • Huang L, Li M, Shao Y, Sun T, Li C, Ma F (2018) Ammonium uptake increases in response to PEG-induced drought stress in Malus hupehensis Rehd. Environ Exp Bot 151:32–42

    Article  CAS  Google Scholar 

  • Joshi R, Anwar K, Das P, Singla-Pareek SL, Pareek A (2017) Overview of methods for assessing salinity and drought tolerance of transgenic wheat lines. In: Wheat biotechnology. Humana Press, New York, pp 83–95

    Chapter  Google Scholar 

  • Kang GZ, Li GZ, Liu GQ, Xu W, Peng XQ, Wang CY, … Guo TC (2013) Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate-glutathione cycle. Biologiaplantarum 57(4):718–724

    CAS  Google Scholar 

  • Kocsy G, Brunner M, Rüegsegger A, Stamp P, Brunold C (1996) Glutathione synthesis in maize genotypes with different sensitivity to chilling. Planta 198:365–370

    Article  CAS  Google Scholar 

  • Kooyers NJ (2015) The evolution of drought escape and avoidance in natural herbaceous populations. Plant Sci 234:155–162

    Article  CAS  PubMed  Google Scholar 

  • Liu T, Sheng M, Wang CY, Chen H, Li Z, Tang M (2015) Impact of arbuscular mycorrhizal fungi on the growth, water status, and photosynthesis of hybrid poplar under drought stress and recovery. Photosynthetica 53(2):250–258

    Article  CAS  Google Scholar 

  • Lu HB, Qiao YM, Gong XC, Li HQ, Zhang Q, Zhao ZH, Meng LL (2015) Influence of drought stress on the photosynthetic characteristics and dry matter accumulation of hybrid millet. Photosynthetica 53(2):306–311

    Article  CAS  Google Scholar 

  • Ma F, Cheng L (2004) Exposure of the shaded side of apple fruit to full sun leads to upregulation of both the xanthophyll cycle and the ascorbate–glutathione cycle. Plant Sci 166(6):1479–1486

    Article  CAS  Google Scholar 

  • Ma NL, Lah WAC, Kadir NA, Mustaqim M, Rahmat Z, Ahmad A et al (2018) Susceptibility and tolerance of rice crop to salt threat: physiological and metabolic inspections. PLoS One 13(2):e0192732

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • McCabe GJ, Wolock DM (2015) Variability and trends in global drought. Earth Space Sci 2(6):223–228

    Article  Google Scholar 

  • Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation. Plant Cell Physiol 33:541–553

    CAS  Google Scholar 

  • Mostofa MG, Hossain MA, Fujita M, Tran LSP (2015) Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice. Sci Rep 5:11433. https://doi.org/10.1038/srep11433

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Munne-Bosch S, Pinto-Marijuan M (2016) Free radicals, oxidative stress and antioxidants. Encycl Appl Plant Sci 2:16–19

    Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880

    CAS  Google Scholar 

  • Nguyen TTT, Klueva N, Chamareck V, Aarti A, Magpantay G, Millena ACM, Nguyen HT (2004) Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Genet Genom 272(1):35–46

    Article  CAS  Google Scholar 

  • Omisun T, Sahoo S, Saha B, Panda SK (2018) Relative salinity tolerance of rice cultivars native to North East India: a physiological, biochemical and molecular perspective. Protoplasma 255(1):193–202

    Article  CAS  PubMed  Google Scholar 

  • Ramel F, Sulmon C, Bogard M, Couée I, Gouesbet G (2009) Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets. BMC Plant Biol 9(1):28

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Rao MV, Davis KR (1999) Ozone-induced cell death occurs via two distinct mechanisms in Arabidopsis: the role of salicylic acid. Plant J 17(6):603–614

    Article  CAS  PubMed  Google Scholar 

  • Rejeb KB, Abdelly C, Savouré A (2014) How reactive oxygen species and proline face stress together. Plant Physiol Biochem 80:278–284

    Article  PubMed  CAS  Google Scholar 

  • Sagisaka S (1976) The occurrence of peroxide in a perennial plant Populasgelrica. Plant Physiol 57:308–309

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saha B, Mishra S, Awasthi JP, Sahoo L, Panda SK (2016a) Enhanced drought and salinity tolerance in transgenic mustard [Brassica juncea (L.) Czern&Coss.] overexpressing Arabidopsis group 4 late embryogenesis abundant gene (AtLEA4-1). Environ Exp Bot 128:99–111

    Article  CAS  Google Scholar 

  • Saha B, Borovskii G, Panda SK (2016b) Alternative oxidase and plant stress tolerance. Plant signal Behav 11(12):e1256530

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sahoo S, Awasthi JP, Sunkar R, Panda SK (2017) Determining glutathione levels in plants. In: Plant stress tolerance. Humana Press, New York, pp 273–277

    Chapter  Google Scholar 

  • Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A (2001) Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127(3):887–898

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sheng M, Tang M, Chen H, Yang B, Zhang F, Huang Y (2008) Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18(6–7):287–296

    Article  CAS  PubMed  Google Scholar 

  • Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53(372):1305–1319

    Article  CAS  PubMed  Google Scholar 

  • Siddiqui MN, Mostofa MG, Akter MM, Srivastava AK, Sayed MA, Hasan MS, Tran LSP (2017) Impact of salt-induced toxicity on growth and yield-potential of local wheat cultivars: oxidative stress and ion toxicity are among the major determinants of salt-tolerant capacity. Chemosphere 187:385–394

    Article  CAS  PubMed  Google Scholar 

  • Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175(2):408–413

    Article  CAS  PubMed  Google Scholar 

  • Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. PhysiologiaPlantarum 121(1):58–65

    CAS  Google Scholar 

  • Sun J, Hu W, Zhou R, Wang L, Wang X, Wang Q et al (2015) The Brachypodiumdistachyon BdWRKY36 gene confers tolerance to drought stress in transgenic tobacco plants. Plant Cell Rep 34(1):23–35

    Article  CAS  PubMed  Google Scholar 

  • Todaka D, Zhao Y, Yoshida T, Kudo M, Kidokoro S, Mizoi J, Toyooka K (2017) Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions. Plant J 90(1):61–78

    Article  CAS  PubMed  Google Scholar 

  • Van Aken O, Van Breusegem F (2015) Licensed to kill: mitochondria, chloroplasts, and cell death. Trends Plant Sci 20(11):754–766

    Article  PubMed  CAS  Google Scholar 

  • Wu Z, Zhao X, Sun X, Tan Q, Tang Y, Nie Z, Hu C (2015) Antioxidant enzyme systems and the ascorbate–glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress. Chemosphere 138:526–536

    Article  CAS  PubMed  Google Scholar 

  • Yang Z, Dai Z, Lu R, Wu B, Tang Q, Xu Y et al (2017) Transcriptome analysis of two species of jute in response to polyethylene glycol (PEG)-induced drought stress. Sci Rep 7(1):16565

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zandalinas SI, Balfagón D, Arbona V, Gómez-Cadenas A (2017) Modulation of antioxidant defense system is associated with combined drought and heat stress tolerance in citrus. Front Plant Sci 8:953

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We highly acknowledge Regional Agricultural Research Station (RARS), Akbarpur (Karimganj), Assam, India; Regional Rainfed Lowland Rice Research Station (RRLRRS) Gerua, Assam, India; and Central Agriculture University (CAU), Imphal, Manipur, India for supplying us with valuable rice seeds.

Author information

Authors and Affiliations

  1. Plant Molecular Biotechnology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India

    Smita Sahoo, Bedabrata Saha, Jay Prakash Awasthi, Takhellambam Omisun, Pankaj Borgohain & Sanjib Kumar Panda

  2. Regional Agricultural Research Station, Assam Agricultural University, Karimganj, Assam, 788710, India

    Safiqul Hussain

  3. Department of Biotechnology, Central University of Rajasthan, Ajmer, Rajasthan, 305817, India

    Jogeswar Panigrahi

Authors
  1. Smita Sahoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bedabrata Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jay Prakash Awasthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takhellambam Omisun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pankaj Borgohain
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Safiqul Hussain
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jogeswar Panigrahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sanjib Kumar Panda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sanjib Kumar Panda.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interest.

Additional information

Communicated by K. Apostol.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sahoo, S., Saha, B., Awasthi, J.P. et al. Physiological introspection into differential drought tolerance in rice cultivars of North East India. Acta Physiol Plant 41, 53 (2019). https://doi.org/10.1007/s11738-019-2841-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-019-2841-x

Keywords

Search

Navigation