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Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH

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Abstract

Multipoint covalent bonding of glucose oxidase (EC 1.1.3.4) to hydrophilic natural polymer dextran and optimization of procedures to obtain, with enhanced temperature and pH stabilities, were studied. Purified enzyme was conjugated with various molecular weight dextrans (17.5, 75, and188 kD) in a ratio of 20:1, 10:1, 1:1, 1:5, 1:10, 1:15, and 1:20. After 1 h of incubation at pH 7, the activities of purified enzyme and conjugates were determined at different temperatures (25°C, 30°C, 35°C, 40°C, 50°C, 60°C, 70°C, and 80°C), and the results were evaluated for thermal resistance. Increases in temperature from 25°C to 50°C did not change the activities of the conjugates. The conjugate, which was prepared with 75 kDa dextran in a molar ratio of 1:5, showed the highest thermal resistance and even the activity still remains at 80°C at pH 7.0. This conjugate also displayed activity in a wide pH range (pH 4.0–7.0) at high temperatures. Conjugate, which was synthesized with 75 kDa dextran in a molar ratio of 1:5, appears to be feasible and useful for biotechnological applications.

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Acknowledgments

This research was supported by grants from T.R. Prime Ministry State Planning Organization (project number 25-DPT-07-04-01) and Yıldız Technical University BAPK (The Production of Medical Biosensors, project number 27-07-04-01).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences and Letters, Yildiz Technical University, Davutpasa Campus, Esenler, 34210, Istanbul, Turkey

    Melda Altikatoglu

  2. Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Davutpasa Campus, Esenler, 34210, Istanbul, Turkey

    Yeliz Basaran, Candan Arioz & Huriye Kuzu

  3. Department of Chemistry, Faculty of Sciences and Letters, Marmara University, Goztepe, 34722, Istanbul, Turkey

    Ayse Ogan

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  1. Melda Altikatoglu
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  2. Yeliz Basaran
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  5. Huriye Kuzu
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Correspondence to Melda Altikatoglu.

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Altikatoglu, M., Basaran, Y., Arioz, C. et al. Glucose Oxidase-dextran Conjugates with Enhanced Stabilities Against Temperature and pH. Appl Biochem Biotechnol 160, 2187–2197 (2010). https://doi.org/10.1007/s12010-009-8812-8

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  • DOI: https://doi.org/10.1007/s12010-009-8812-8

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