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Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum

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Abstract

The efficacy of seven strains of Trichoderma asperellum collected from the fields in Southern China was assessed against Fusarium graminearum (FG) the causal agent of corn stalk rot of maize were in vitro for their antagonistic properties followed by statistical model of principal compound analysis to identify the beneficial antagonist T. asperellum strain. The key factors of antagonist activity were attributed to a total of 13 factors including cell wall degrading enzymes (chitnase, protease and β-glucanases), secondary metabolites and peptaibols and these were analyzed from eight strains of Trichoderma. A linear regression model demonstrated that interaction of enzymes and secondary metabolites of T. asperellum strain ZJSX5003 enhanced the antagonist activity against FG. Further, this strain displayed a disease reduction of 71 % in maize plants inoculated with FG compared to negative control. Pointing out that the T. asperellum strain ZJSX5003 is a potential source for the development of a biocontrol agent against corn stalk rot.

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Acknowledgments

We thank the financial support from the Special Project of Basic Work for Science and Technology (2014FY120900), the key project of the basic research of Shanghai Municipal Science and Technology Commission (12JC1404600), Natural Science Foundation of China (No. 31201557), Natural Science Fund of Shanghai (No.12ZR1414100), the special fund of Modern Agricultural Industry Technology System (CARS-02), Ministry of Education University Doctoral Foundation (No. 20120073120070), and the SJTU Medical-Engineering Cross Research Fund (No. YG2015MS37).

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Authors and Affiliations

  1. Department of Environment and Resource, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240, People’s Republic of China

    Yaqian Li, Ruiyan Sun, Jia Yu, Kandasamy Saravanakumar & Jie Chen

  2. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People’s Republic of China

    Yaqian Li, Ruiyan Sun, Jia Yu, Kandasamy Saravanakumar & Jie Chen

  3. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, People’s Republic of China

    Yaqian Li, Ruiyan Sun, Jia Yu, Kandasamy Saravanakumar & Jie Chen

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  1. Yaqian Li
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Correspondence to Jie Chen.

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Li, Y., Sun, R., Yu, J. et al. Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum . Indian J Microbiol 56, 318–327 (2016). https://doi.org/10.1007/s12088-016-0581-9

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