Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Skip to main content
Springer Nature Link
Log in

Effect of Drought Stress on Anatomical Structure and Chloroplast Ultrastructure in Leaves of Sugarcane

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

In order to provide a reference for investigating the mechanism of drought resistance in sugarcane, variations of chlorophyll content and chloroplast ultrastructure in sugarcane leaves were analyzed. The present research was conducted using sugarcane cultivars, strongly drought-resistant F172 and weakly drought-resistant YL6, as plant materials in pot experiment under controlled greenhouse condition. At elongation stage, the plants were provided different degrees of drought stress: (1) mild drought with 65–70 % of the soil water capacity; (2) moderate drought with 45–65 % of the soil water content; (3) severe drought with 25–45 % of soil water capacity and; (4) control with 70 % of soil water capacity. Chlorophyll content in leaves was measured, and variations of green leaves number and chloroplast ultrastructure were observed. It was found that the green leaves of sugarcane and chlorophyll content were significantly reduced in the process of drought stress. Upper and lower cuticle thickness was getting thickened during drought stress, but the thickness of lower epidermal cuticle of YL6 variety was reduced under severe drought condition. Ultrastructure observation showed that, in most cases, the chloroplasts were close to the cell wall and well arranged, and the chloroplast thylakoids were orderly arranged in the chloroplasts. With the ongoing of drought stress, plasmosis occurred, the chloroplasts moved closer to the center of the cell, and turned gradually from long oval to nearly round, and starch grains increased. Under severe drought conditions, F172 still maintained integrity of the chloroplast structure while the chloroplast in YL6 were severely deformed and became blurred in shape.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Anderson, J.M. 1973. Composition of the photo systems and chloroplast structure in extreme shade plants light-dependent reversal of dark-chilling induced changes in chloroplast structure and arrangement of chlorophyll-protein complexes in bean thylakoid membranes. Biophysica Acta 325: 573–585.

    Article  CAS  Google Scholar 

  • Bai, Z.Y., C.D. Li, and P. Qu. 2009. Effect of drought stress on ultrastructure of flag leaves in wheat chromosome substitution lines. Journal of Chinese Electron Microscopy Society 28(1): 68–73.

    Google Scholar 

  • Baranova, A.M. 1987. Historical development of the present classification of morphological type of stomatas. The Botanical Review 53(1): 53–79.

    Article  Google Scholar 

  • Boyer, T.S., S.C. Wong, and D. Farquhar. 1997. CO2 and water vapor exchange across leaf cuticle (epidermis) at various water potentials. Plant Physiology 114: 185–191.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Chaitanya, K.V., P.P. Jutur, D. Sundar, and A. Ramachandra Reddy. 2003. Water stress effects on photosynthesis in different mulberry cultivars. Plant Growth Regulation 40: 75–80.

    Article  CAS  Google Scholar 

  • Chen, J.H., R.H. Li, P.G. Guo, Y.S. Xian, C.E. Tian, and S.Y. Miu. 2011. Impact of drought stress on the ultrastructure of leaf cells in three Barley genotypes differing in level of drought tolerance. Chinese Bulletin of Botany 46(1): 28–36.

    Article  Google Scholar 

  • Griffiths, H., and M.A.J. Parry. 2002. Plant responses to water stress. Annals of Botany 89: 801–802.

    Article  PubMed  Google Scholar 

  • Gui, Y.Y., R.Z. Yang, and H. Zhou. 2009. Physiological response of sugarcane in different water stress and water recovery condition and drought-resistance identification. Guangdong Agricultural Sciences 9: 19–21.

    Google Scholar 

  • Guo, W.H., B. Li, Y.M. Huang, H.X. Zhao, and X.S. Zhang. 2003. Effects of different water stress on eco-physiological characteristics of Hippophae rhamnoides seedlings. Acta Botanica Sinica 45(10): 1238–1244.

    Google Scholar 

  • Jian, L.C., and H. Wang. 2009. Stress plant cell biology, 159–167. Beijing: Science Press.

    Google Scholar 

  • Jin, W., L.T. Yang, P. Ying, J.B. Yang, C.Y. Luo, and Y.R. Li. 2012. Eco-physiological responses of different sugarcane varieties to drought and re-watering. Journal of Southern Agriculture 43: 1945–1951.

    CAS  Google Scholar 

  • Lawlor, D.W. 2002. Limitation to photosynthesis in water-stressed leaves: Stomata vs. metabolism and the role of ATP. Annals of Botany 89: 871–885.

    Article  CAS  PubMed  Google Scholar 

  • Li, S.L. 2011. Response mechanism of different cold sensitive sugarcane cultivars to low temperature stress. Ph.D. dissertation of Guangxi University, Nanning, 19–34.

  • Li, Y.R., and L.T. Yang. 2009. New developments in sugarcane industry and technologies in China since 1990. Southwest China Journal of Agricultural Sciences 22(5): 1469–1476.

    Google Scholar 

  • Li, Y.R. 2006. Research and development strategies to improve sugar productivity in China. In Technologies to improve sugar productivity in developing countries, ed. Y.R. Li, and S. Solomon, 7–14. Beijing: China Agriculture Press.

    Google Scholar 

  • Li, Y.R. 2010. Modern sugarcane science, 1–22. Beijing, China: China Agriculture Press.

    Google Scholar 

  • Li, Z.L., and X.Y. Zhang. 1983. Plant anatomy, 261–262. Beijing: Higher Education Press.

    Google Scholar 

  • Lu, J., and L. Wang. 2001. Problems and solutions in paraffin production. Preclinical Medical Education Edition 3(3): 263.

    Google Scholar 

  • Malik, K.B. 1986. Some anatomical characteristics of sugarcane varieties in relation to drough resistance. Agriculture Research 11(1): 43–49.

    Google Scholar 

  • Menese, R.S. 1985. Assessment of drought resistance in sugarcane varieties through the determination of the release of electrolytes. ATAC 14(6): 11–16.

    Google Scholar 

  • Ming, H., C.S. Hu, Y.M. Zhang, and Y.S. Cheng. 2007. Improved extraction methods of chlorophyll from maize. Journal of Maize Sciences 15(4): 93–95.

    Google Scholar 

  • Mo, J.R., and C.S. Zhou. 1984. Physiological bases for sugarcane cultivation and breeding, 329–334. Fuzhou, China: Fujian Science and Technology Press.

    Google Scholar 

  • Øllgaard, H., and J. Räsänen. 2008. Six new Taraxacum species (Asteraceas) from Finland and adjacent countries. Annales Botanici Fennici 45: 375–385.

    Article  Google Scholar 

  • Pan, C.E., L.P. Tian, Z.Z. Li, T.Y. Zhang, and P.C. Li. 2011. Studies on drought resistance on anatomical structure of leaves of 5 poplar clones. Chinese Agricultural Science Bulletin 27(2): 21–25.

    Google Scholar 

  • Pu, H.L. 1990. Anatomy and physiology characteristics drought preliminary study in Apricot, pear leaf. Gansu Agricultural Science and Technology 2: 14–16.

    Google Scholar 

  • Spurr, A.R. 1969. A low viscosity epoxy resin embedding medium for electron microscopy. Journal Ultrastructure Research 26: 31–43.

    Article  CAS  Google Scholar 

  • Tan, Y.M. 1988. Fatty acids and permeable relationship with the drought resistance in sugarcane leaves. Journal of Fujian Agricultural College 17(3): 211–215.

    CAS  Google Scholar 

  • Wang, G.F., L.G. Li, X.Y. Li, S.Y. Du, L.Y. Zhang, and L.F. Xie. 2006. Relationship between the morphological characteristics of leaf surface and drought resistance of sea buckthorn. Acta Horticulturae Sinica 33(6): 1310–1312.

    Google Scholar 

  • Wang, J. 1998. Biological techniques. Yinchuan, China: Ningxia People’s Education Press.

    Google Scholar 

  • Wang, Y.J. 2009. Statistics analysis on the quantitative characteristics of bulliform cells in wheat leaves. Journal of Triticeae Crops 29(6): 1100–1104.

    Google Scholar 

  • Xu, Y.S. 1986. Sugarcane callus physiological responses to water stress and drought resistance. Guangxi Agricultural College Thesis, Nanning.

  • Yu, H.Q., X.L. Wang, C.J. Jiang, X.G. Wang, and M.J. Cao. 2008. Injured process on anatomical structure of maize seedling under soil drought. Agricultural Research in the Arid Areas 26(5): 143–147.

    Google Scholar 

  • Yu, M., C.X. Hu, and Y.H. Wang. 2005. Effect of Mo deficiency on the content of chlorophyll and the ultrastructure of chloroplast in winter wheat cultivars. Journal of Huazhong Agricultural University 24(5): 465–469.

    CAS  Google Scholar 

  • Zheng, W.J., G.C. Chen, C.L. Zhang, Y.X. Hu, L.H. Li, and J.X. Lin. 2002. Physiological adaptation of habitat by ion distribution in the leaves of four ecotypes of reed. Acta Botanica Sinica 44(1): 82–87.

    Google Scholar 

  • Zhong, X.Q., and Z.B. Ye. 1992. Changes of the ultrastructure in sugarcane leaves after the drought. Journal of South China Agricultural University 13(3): 64–68.

    Google Scholar 

  • Zhu, L.H., Y.X. Xing, L.T. Yang, Y.R. Li, R.Z. Yang, and L.X. Mo. 2010. Effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling. Journal of Anhui Agricultural Sciences 38(23): 12570–12573.

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National High Technology Research and Development Program of China (863 Program),(2013AA102604), International Cooperation Program project of China (2013DFA31600), Guangxi Funds for Bagui Scholars and Distinguished Visiting Experts, Guangxi Natural Science Fund (2011GXNSFF018002, 2012GXNSFDA053011), Guangxi R & D Program project (GKC1123008-1), Science and Technology Development Foundation of Guangxi Academy of Agricultural Sciences (2011YT01), Guangxi Key Laboratory of Sugarcane Genetic Improvement Grant (12-K-03-01).

Author information

Authors and Affiliations

  1. College of Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, China

    Feng-Juan Zhang, Kun-Kun Zhang, Cheng-Zhong Du, Jian Li, Yong-Xiu Xing, Li-Tao Yang & Yang-Rui Li

  2. Sugarcane Research Center, Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning, 530007, China

    Yong-Xiu Xing, Li-Tao Yang & Yang-Rui Li

Authors
  1. Feng-Juan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kun-Kun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cheng-Zhong Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yong-Xiu Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Li-Tao Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yang-Rui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Li-Tao Yang or Yang-Rui Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, FJ., Zhang, KK., Du, CZ. et al. Effect of Drought Stress on Anatomical Structure and Chloroplast Ultrastructure in Leaves of Sugarcane. Sugar Tech 17, 41–48 (2015). https://doi.org/10.1007/s12355-014-0337-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-014-0337-y

Keywords

Search

Navigation