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Production and Characterisation of Xylanase and Endoglucanases Produced by Penicillium roqueforti ATCC 10110 Through the Solid-State Fermentation of Rice Husk Residue

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Abstract

Purpose

Penicillium roqueforti ATCC 10110 was cultivated in rice husk residue, to produce a multienzymatic extract, which was characterised for its potential biotechnological applications.

Methods

Optimisation of the fermentation conditions for the xylanase activity production (U/g) was evaluated by using a Doehlert-type experimental design.

Results

The optimum xylanase activity (at 32 °C/82 h), was 1.04 U/g, which represented a deviation of 3% from the theoretically optimised value predicted by the quadratic model (R2 = 0.92). The optimum conditions were observed at pH 7.0 and 35 °C. The xylanase activity was favoured, particularly, by the presence (1 M) of Co2+ and Cu2+ and the kinetic constants were determined (K m  = 7.22 mg/ml and v max  = 3.29 U/g). For the endoglucanase activity, it was not possible to adjust a quadratic model but maximal activities (2.37 ± 0.01 U/g) were obtained at 32 °C for 72 h. For this enzyme, the optimum conditions were pH 4.8 and 50 °C. Also, Co2+, Cu2+, acetone, ethanol and isopropanol increased the endoglucanase activity.

Conclusion

The substrate rice husk, without any additives, permitted the acquisition of xylanases and endoglucanases similar to those obtained from synthetic substrates, justifying its application as a substrate for solid-state fermentations.

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Acknowledgements

Authors would like to acknowledge the Banco do Nordeste do Brasil (BNB, Brazil), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), the Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for their important financial support.

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Authors and Affiliations

  1. Department of Exact Sciences and Technology, State University of Santa Cruz (UESC), Ilhéus, 45654-370, Brazil

    George Lima Marques

  2. Department of Basic and Instrumental Studies, State University of Southwest Bahia (UESB), Itapetinga, 45700-000, Brazil

    Nadabe dos Santos Reis

  3. Department of Exact Sciences and Technology, State University of Santa Cruz (UESC), Ilhéus, 45654-370, Brazil

    Tatielle Pereira Silva

  4. Department of Exact Sciences and Technology, State University of Santa Cruz – (UESC), Ilhéus, 45654-370, Brazil

    Marcio Luiz Oliveira Ferreira, Elizama Aguiar-Oliveira, Julieta Rangel de Oliveira & Marcelo Franco

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  1. George Lima Marques
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Correspondence to Marcelo Franco.

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Marques, G.L., dos Santos Reis, N., Silva, T.P. et al. Production and Characterisation of Xylanase and Endoglucanases Produced by Penicillium roqueforti ATCC 10110 Through the Solid-State Fermentation of Rice Husk Residue. Waste Biomass Valor 9, 2061–2069 (2018). https://doi.org/10.1007/s12649-017-9994-x

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