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Occurrence and toxigenicity of shape Fusarium moniliforme from freshly harvested maize ears with special references to fumonisin production in Egypt

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Abstract

Using the seed-plate technique, 18 different isolates of Fusarium moniliforme were isolated on pentachloronitrobenzene (PCNB) agar medium from 18 samples of a local variety of corn collected from locations in Minia Governorate.

The isolates of F moniliforme were screened for their ability to produce fumonisins on polished rice grains using the solid state fermentation technique. Based on thin layer chromatographic (TLC) analyses using silica gel plates, 14 of the 18 isolates tested produced FB1 and FB2 with R f (0.17) and (0.24), respectively.

Concentration of FB1 was estimated using high performance liquid chromatography (HPLC). Production of FB1 by the 14 isolates of F. moniliforme tested ranged from 69 to 4495 ppm indicating that mouldy corn may represent a health hazard to consumers.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Minia University, Minia, Egypt

    Ezzat M. Fadl

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  1. Ezzat M. Fadl
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Fadl, E.M. Occurrence and toxigenicity of shape Fusarium moniliforme from freshly harvested maize ears with special references to fumonisin production in Egypt. Mycopathologia 140, 99–103 (1997). https://doi.org/10.1023/A:1006874029950

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