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Protein microarrays and proteomics

Nature Genetics volume 32, pages 526–532 (2002)Cite this article

Abstract

The system-wide study of proteins presents an exciting challenge in this information-rich age of whole-genome biology. Although traditional investigations have yielded abundant information about individual proteins, they have been less successful at providing us with an integrated understanding of biological systems. The promise of proteomics is that, by studying many components simultaneously, we will learn how proteins interact with each other, as well as with non-proteinaceous molecules, to control complex processes in cells, tissues and even whole organisms. Here, I discuss the role of microarray technology in this burgeoning area.

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Figure 1: The spectrum of protein analysis, ranging from traditional biochemistry to unbiased proteomics.
Figure 2: Current immunoassay strategies used in protein-detecting microarrays.

Bob Crimi

Figure 3: Complications arising from regulated protein–protein interactions.

Bob Crimi

Figure 4: Proof-of-concept experiments showing the detection of protein–protein interactions on a model protein function microarray37.
Figure 5: A 'proteome chip' composed of 6,566 protein samples representing 5,800 unique proteins, which are spotted in duplicate on a single nickel-coated glass microscope slide39.

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  1. Department of Chemistry and Chemical Biology, and Bauer Center for Genomics Research, Harvard University, 12 Oxford Street, Cambridge, 02138, Massachusetts, USA

    Gavin MacBeath

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MacBeath, G. Protein microarrays and proteomics. Nat Genet 32 (Suppl 4), 526–532 (2002). https://doi.org/10.1038/ng1037

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