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Acknowledgements
We thank G. Baron, V. Sim and L. Taubner for critical review of this manuscript. We thank K. Meade-White and B. Race (Rocky Mountain Laboratories) for the CSF samples, G. Raymond (Rocky Mountain Laboratories) for the brain tissue samples, and G. Hettrick and A. Mora for graphics assistance. This research was supported in part by the Intramural Research Program of the US National Institute of Allergy and Infectious Diseases, National Institutes of Health.
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R.A., R.A.M., S.A.P. and B.C. are authors on US patent applications 60/961,364 and 61/021,865, which cover the detection of prions by seeded conversion of recombinant prion protein.
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Atarashi, R., Wilham, J., Christensen, L. et al. Simplified ultrasensitive prion detection by recombinant PrP conversion with shaking. Nat Methods 5, 211â212 (2008). https://doi.org/10.1038/nmeth0308-211
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DOI: https://doi.org/10.1038/nmeth0308-211