Abstract
A water-soluble fluorescence dye, bis(2,2′-bipyridine)-5-amino-1,10-phenanthroline ruthenium complex (Ru(bpy)2-(5-NH2-1,10-phen)), was synthesized and used as a fluorescence probe for detecting dissolved oxygen in water. The fluorescence intensity of the probe in different dissolved-oxygen concentrations was investigated. The sensitivity of the probe was evaluated in terms of the ratio ####, where #### and #### correspond to the detected fluorescence intensity of nitrogen and oxygen-saturated solutions, respectively. The experimental results showed that the probe yielded a linear Stern-Volmer plot, and had a #### ratio of about 5.2. The detection limit, defined as three-times the standard deviation, was 8.6 × 10−7 mol L−1 after eleven determinations of nitrogen-saturated blank solutions. Additionally, the probe was pH-insensitive and ionic strength-independent with good characteristics of practicality and selectivity.
Similar content being viewed by others
References
G. Mistlberger, K. Koren, S. M. Borisov, and I. Klimant, Anal. Chem., 2010, 82, 2124.
P. C. Thomas, M. Halter, A. Tona, S. R. Raghavan, A. L. Plant, and S. P. Forry, Anal. Chem., 2009, 81, 9239.
L. Hutton, M. E. Newton, P. R. Unwin, and J. V. Macpherson, Anal. Chem., 2008, 81, 1023.
A. J. Palma, J. López-González, L. J. Asensio, M. D. Fernández-Ramos, and L. F. Capitán-Vallvey, Anal. Chem., 2007, 79, 3173.
S. Sasaki, Anal. Sci., 2006, 22, 903.
ISO 5813, Water Quality—Determination of Dissolved Oxygen—Iodometric Method, 1983.
W. Luo, M. E. Abbas, L. Zhu, W. Zhou, K. Li, H. Tang, S. Liu, and W. Li, Anal. Chim. Acta, 2009, 640, 63.
R. Ramamoorthy, P. Dutta, and S. Akbar, J. Mater. Sci., 2003, 38, 4271.
T. Sakai, H. Takio, N. Teshima, and H. Nishikawa, Anal. Chim. Acta, 2001, 438, 117.
C. Baleizão, S. Nagl, M. Schäferling, M. N. Berberan-Santos, and O. S. Wolfbeis, Anal. Chem., 2008, 80, 6449.
J. R. Lakowicz, “Principles of Fluorescence Spectroscopy”, 2nd ed., 1999, Kluwer Academic/Plenum Press, New York, 240.
P. J. R. Roche, M. C. K. Cheung, K. Y. Yung, A. G. Kirk, V. P. Chodavarpu, and F. V. Bright, Sens. Actuators, B, 2010, 147, 581.
O. Oter, K. Ertekin, and S. Derinkuyu, Mater. Chem. Phys., 2009, 113, 322.
H. J. Kim, Y. C. Jeong, and J. I. Rhee, Talanta, 2008, 76, 1070.
P. A. S. Jorge, C. Maule, A. J. Silva, R. Benrashid, J. L. Santos, and F. Farahi, Anal. Chim. Acta, 2008, 606, 223.
C. S. Chu and Y. L. Lo, Sens. Actuators, B, 2008, 134, 711.
K. Fujimori, N. Takenaka, H. Bandow, and Y. Maeda, Anal. Sci., 2001, 17, 161.
J. W. Dobrucki, J. Photochem. Photobiol., B, 2001, 65, 136.
H. Zhang, B. Li, B. Lei, W. Li, and S. Lu, Sens. Actuators, B, 2007, 123, 508.
Z. Rosenzweig and R. Kopelman, Anal. Chem., 1995, 67, 2650.
X. Xiong, D. Xiao, and M. M. F. Choi, Sens. Actuators, B, 2006, 117, 172.
X. M. Chen, G. H. Wu, J. M. Chen, Y. Q. Jiang, G. N. Chen, M. Oyama, X. Chen, and X. R. Wang, Biosens. Bioelectron., 2010, 26, 872.
Y. Tao, Z. J. Lin, X. M. Chen, X. L. Huang, M. Oyama, X. Chen, and X. R. Wang, Sens. Actuators, B, 2008, 129, 758.
S. Kemp, N. J. Wheate, D. P. Buck, M. Nikac, J. G. Collins, and J. R. Aldrich-Wright, J. Inorg. Biochem., 2007, 101, 1049.
C. D. Ellis, L. D. Margerum, R. W. Murray, and T. J. Meyer, Inorg. Chem., 1983, 22, 1283.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Hu, L., Niu, C., Zeng, G. et al. Determination of Dissolved Oxygen in Water Based on Its Quenching Effect on the Fluorescent Intensity of Bis(2,2′-bipyridine)-5-amino-1,10-phenanthroline Ruthenium Complex. ANAL. SCI. 27, 1121–1125 (2011). https://doi.org/10.2116/analsci.27.1121
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.27.1121