Abstract
The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.
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Acknowledgments
This work was supported in part by the NEDO Industrial Technology Research Assistance Project, Shimadzu Science Foundation, Asahi Glass Foundation, and a Grant-in-Aid for Scientific Research (JSPS, KAKENHI; Grant Numbers 15K15199, 24350035, and 15H03823).
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Sato, K., Kikuchi, S., Yoshida, E. et al. Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone. ANAL. SCI. 32, 113–116 (2016). https://doi.org/10.2116/analsci.32.113
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DOI: https://doi.org/10.2116/analsci.32.113