Abstract
Confocal laser scanning microscopy was utilized to compare the chloroplast morphology and ontogeny among five strains of the green alga Asterochloris. Parsimony analysis inferred from the rDNA ITS sequences confirmed their placement in three distinct lineages: Asterochloris phycobiontica, Trebouxia pyriformis and Asterochloris sp. Examination by confocal microscopy revealed the existence of interspecific differences in the chloroplast ontogeny of Asterochloris; this was based upon either specific chloroplast structures observed in a single species, or on the differential timing of particular ontogenetic sequences. The occurrence of flat parietal chloroplasts prior to cell division, considered as a basic morphological discriminative character of Asterochloris, was clearly associated with the process of aplanosporogenesis. By contrast, chloroplast transformation prior to the formation of autospores proceeded simply by the multiple fission of the chloroplast matrix in the cell lumen.
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References
Abramoff M.D., Magelhaes P.J. & Ram S.J. 2004. Image processing with Image J. Biophoton. Int. 11: 36–42.
Ahmadjian V. 1960. Some new and interresting species of Trebouxia, a genus of lichenized algae. Am. J. Bot. 47: 677–683.
Ahmadjian V. 1993. The lichen symbiosis. John Wiley & Sons, New York, NY, USA, 250 pp.
Beck A., Friedl T. & Rambold G. 1998. Selectivity of photobiont choice in a defined lichen community: inferences from cultural and molecular studies. New Phytol. 139: 709–720.
Bischoff H.W. & Bold H.C. 1963. Phycological Studies. IV. Some soil algae from enchanted rock and related algal species. Univ. Texas Publ. 6318: 1–95.
DePriest P.T. 2004. Early molecular investigations of lichenforming symbionts: 1986–2001. Annu. Rev. Microbiol. 58: 273–301.
Doyle J.J. & Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Friedl T. 1995. Inferring taxonomic positions and testing genus level assignments in coccoid green lichen algae: A phylogenetic analysis of 18S ribosomal RNA sequences from Dictyochloropsis reticulata and from members of the genus Myrmecia (Chlorophyta, Trebouxiophyceae Cl. Nov.). J. Phycol. 31: 632–639.
Friedl T. & Büdel B. 1996. Photobionts, pp. 8–23. In: Nash T.H. (ed.), Lichen biology. Cambridge University Press, Cambridge, UK.
Friedl T. & Gärtner G. 1988. Trebouxia (Pleurastrales, Chlorophyta) as a phycobiont in the lichen genus Diploschistes. Arch. Protistenkd. 135: 147–158.
Friedl T. & Rokitta C. 1997. Species relationships in the lichen alga Trebouxia (Chlorophyta, Trebouxiophyceae): Molecular phylogenetic analyses of nuclear-encoded large subunit rRNA gene sequences. Symbiosis 23: 125–148.
Friedl T. & Zeltner C.T. 1994. Assessing the relationships of some coccoid green lichen algae and the Microthamniales (Chlorophyta) with 18S ribosomal RNA gene sequence comparisons. J. Phycol. 30: 500–506.
Gärtner G. 1985. Die Gattung Trebouxia Puymaly (Chlorellales, Chlorophyceae). Arch. Hydrobiol. Suppl., Algol. Stud. 41: 495–548.
Hesse M. 2001. Zum Gedenken an Frau emer. O. Prof. Dr. Elisabeth Woess. Verh. Zool.-Bot. Ges. Österreich 138: 275–278.
Hildreth K.C. & Ahmadjian V. 1981. A study of Trebouxia and Pseudotrebouxia isolates from different lichens. Lichenologist 13: 65–86.
Kroken S. & Taylor J.W. 2000. Phylogenetic species, reproductive mode, and specifity of the green alga Trebouxia forming lichens with the fungal genus Letharia. Bryologist 103: 645–660.
Kumar S., Tamura K. & Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinform. 5: 150–163.
Neustupa J., Eliáš M. & Šejnohová L. 2007. A taxonomic study of two Stichococcus species (Trebouxiophyceae, Chlorophyta) with a starch-enveloped pyrenoid. Nova Hedwigia 84: 51–63.
Piercey-Normore M.D. & DePriest P.T. 2001. Algal switching among lichen symbioses. Am. J. Bot. 88: 1490–1498.
Rambold G., Friedl T. & Beck A. 1998. Photobionts in lichens: Possible indicators of phylogenetic relationships? Bryologist 101: 392–397.
Schwendener S. 1867. Über die wahre Natur der Flechtengonidien. Verh. Schweiz. Naturforsch. Ges. 9–11.
Škaloud P., Neustupa J., Radochová B. & Kubínová L. 2005. Confocal microscopy of chloroplast morphology and ontogeny in three strains of Dictyochloropsis (Trebouxiophyceae, Chlorophyta). Phycologia 44: 261–269.
Swofford D.L. 2003. PAUP*. Phylogenetic analysis using parsimony (and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997. The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acid. Res. 25: 4876–4882.
Tschermak-Woess E. 1980. Asterochloris phycobiontica, gen. at spec., nov., der Phycobiont der Flechte Varicellaria carneonivea. Pl. Syst. Evol. 135: 279–294.
Tschermak-Woess E. 1988. The algal partner, pp. 39–92. In: Galun M. (ed.), CRC Handbook of Lichenology, Vol. 1., CRC-Press, Boca Raton, Florida.
Tschermak-Woess E. 1989. Developmental studies in trebouxioid algae and taxonomical consequences. Pl. Syst. Evol. 164: 161–195.
White T.J., Bruns T., Lee S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 345–322. In: Innis M.A., Gelfand D.H., Sninsky J.J. & White T.J. (eds), PCR Protocols: A Guide to Methods and Applications, Academic Press, San Diego.
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Škaloud, P., Peksa, O. Comparative study of chloroplast morphology and ontogeny in Asterochloris (Trebouxiophyceae, Chlorophyta). Biologia 63, 873–880 (2008). https://doi.org/10.2478/s11756-008-0115-y
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DOI: https://doi.org/10.2478/s11756-008-0115-y